Data on characterization of glass biochips and validation of the label-free biosensor for detection of autoantibodies in human serum.

The data represent in-depth characterization of a novel method for highly sensitive simultaneous measuring in human serum of both critical parameters of autoantibodies: concentration and native kinetics. The latter refers to autoantibody interaction with free, not immobilized, antigen. The method and related biosensors are based on the spectral-correlation and spectral-phase interferometry. The data cover: multi-factor optimization and quantitative characterization of the developed affordable single-used biochips, including X-ray photoelectron spectroscopy (XPS) control of chemical modifications of the surface during fabrication; antibody screening; optimization and verification of protocols for label-free biosensing in human serum; mathematical model for fitting experimental data and calculation of kinetic constants of interaction of autoantibodies with free antigen; comprehensive verification of the method specificity; correlation between the data obtained with the developed biosensor and with enzyme linked immunosorbent assay (ELISA); comparison of analytical characteristics of the developed biosensor with the most advanced label-based methods. The data importance is confirmed by a companion paper (DOI 10.1016/j.bios.2020.112187), which shows that the combination of mentioned autoantibody parameters is promising for more accurate criteria for early diagnostics and efficient therapy of autoimmune disorders. The obtained data can be used in development of a wide range of biosensors, both label-free and based on various labels.

[Pericarditis in contemporary therapeutic clinic: nosological spectrum, approaches to diagnosis and treatment].

AIM: To analyze the register of pericarditis in a therapeutic clinic, to evaluate their nosological spectrum, to optimize approaches to diagnosis and treatment. MATERIALS AND METHODS: For the period 20072018, the register includes 76 patients with the diagnosis of pericarditis (average age 53.115.7 years, 2085 years, 46 female). Patients with hydropericardium were not included in the register. Diagnostic puncture of pericardium was carried out in 5 patients, pleural puncture in 11 patients. Morphological diagnostics included endomyocardial/ intraoperative biopsy of myocardium (n=4/2), thoracoscopic/intraoperative biopsy of pericardium (n=1/6), pleural puncture (n=5), transbronchial (n=1), thoracoscopic biopsy of intrathoracic lymph nodes (n=2), lung (n=1), supraclavicular lymph node biopsy (n=1), salivary gland (n=1), subcutaneous fat and rectum biopsy per amyloid (n=6/1). The genome of cardiotropic viruses, level of anti-heart antibodies, C-reactive protein, antinuclear factor, rheumatoid factor (antibodies to cyclic citrullinized peptide), antibodies to neutrophil cytoplasm were determined, extractable nuclear antigens (ENA), protein immunoelectrophoresis, diaskin test, computed tomography of lungs and heart, cardiac magnetic resonance imaging, oncologic search. RESULTS: The following forms of pericarditis were verified: tuberculosis (14%, including in combination with hypertrophic cardiomyopathy HCM), acute / chronic viral (8%) and infectious immune (38%), including perimyocarditis in 77%, pericarditis associated with mediastinum lymphoma/sarcoma (4%), sarcoidosis (3%), diffuse diseases of connective tissue and vasculitis (systemic lupus erythematosus, rheumatoid arthritis, diseases of Horton, Takayasu, Shegren, Wegener, 12%), leukoclastic vasculitis, Loefflers endomyocarditis, AL-amyloidosis, thrombotic microangiopathy (1% each), HCM (8%), coronary heart disease (constriction after repeated punctures and suppuration; postinfection and immune, 4%), after radiofrequency catheter ablation and valve prosthetics (2%). Tuberculosis was the main causes of constrictive pericarditis (36%). Treatment included steroids (n=39), also in combination with cytostatics (n=12), anti-tuberculosis drugs (n=9), acyclovir/ganclovir (n=14), hydroxychloroquine (n=23), colchicine (n=13), non-steroidal anti-inflammatory drugs (n=21), L-tyroxine (n=5), chemotherapy (n=1). In 36 patients different types of therapy were combined. Treatment results observed in 55 patients. Excellent and stable results were achieved in 82% of them. Pericardiectomy/pericardial resection was successfully performed in 8 patients. Lethality was 13.2% (10 patients) with an average follow-up 9 [2; 29.5] months (up to 10 years). Causes of death were chronic heart failure, surgery for HCM, pulmonary embolism, tumor. CONCLUSION: During a special examination, the nature of pericarditis was established in 97% of patients. Morphological and cytological diagnostics methods play the leading role. Tuberculosis pericarditis, infectious-immune and pericarditis in systemic diseases prevailed. Infectious immune pericarditis is characterized by small and medium exudate without restriction and accompanying myocarditis. Steroids remain the first line of therapy in most cases. Hydroxychloroquine as well as colchicine can be successfully used in moderate / low activity of immune pericarditis and as a long-term maintenance therapy after steroid stop.

[THE AGE AND WORK EXPERIENCE DYNAMICS OF INDICES OF HEALTH OF EMPLOYEES--CRITERIA FOR COMPARISON OF OCCUPATIONAL AND NONOCCUPATIONAL RISKS].

There are reported data of the analysis of parameters of the general morbidity (hypertension, chronic diseases, organs of respiratory and digestive system, adiposity, vertebral osteochondrosis) in dependence on age and work experience of dockers-mechanizators in seaport at various working conditions. Morbidity rate indices were calculated on results of the profound medical examination at annual periodic surveys of workers. There were analyzed ecological and ergonomic indices of working conditions, including an estimation of physiological intensity of the work. The general hygienic assessment of working conditions was executed on the basis of the measurement of rates of gains in the risk of diseases depending on age and the experience of work (an annual gain of risk). The divergence of these rates was used for the determination of occupational and nonoccupational fractions of the general morbidity in employees working in harmful conditions.

[Evaluation of occupationally conditioned and non-occupational risks of health disorders in truck drivers].

The authors present analysis of changes in genera! morbidity related to age and length of service for truck drivers (chronic diseases of cardiovascular system, respiratory and digestive organs, urinary system, spine, locomotory system and connective tissue) exposed to complex combination of occupational hazards (unusual work schedule, night shifts, air pollution with automobile transport exhaust gases). Morbid "ity parameters were calculated according to deep medical investigation results in periodic examinations of drivers and their appealability to medical office. General hygienic evaluation of work conditions was performed according to increment in risk of diseases for length of service years (annual risk increase). Difference of disease risk increase rates related to age and length of service was applied to determine occupational and non-occupational fractions of general morbidity in drivers working in hazardous conditions.

[Evaluating occupational risk in night shift work schedule].

The authors analyzed and summarized risk values of acute and chronic health disorders in night shift workers. Average annual risk increments for various chronic diseases in night shift workers were calculated. Based on these data, the authors conclude on hazard degree of night shift work.

[The stimulation of reparative regeneration in the treatment of fractures of the extremities with the use of new biotechnologies].

The paper presents the results of a study of Russian and foreign authors on modern methods of stimulation of reparative regeneration of bone tissue. Highlight general principles for the construction and use of bioplastic materials. An attempt to classify the most famous of biomaterials based on their physico-chemical and biological properties, as well as identified trends in the further development of tissue engineering and its importance in traumatology and orthopedics.

[Physiological and hygienic grounding 8-hour and 12-hour shifts at process production].

On workplaces of an oil refining factory for an estimation of 8-hour and 12-hour shifts the criterion of tiresomeness of work - speed of decrease in working capacity on hours on hours of the working day. Conditions of the industrial environment, physiological intensity of work (density and rate of labour actions), dynamics of parameters of a functional state of an organism of workers, data about symptoms of acute and chronic weariness were analyzed. It is supposed, that at absence of harmful factors of the industrial environment the opportunity of lengthening of a work day till 12-hour is defined by physiological intensity of work - a combination of duration of the working day and week with density and rate of labour actions.

[Pressing problems of labor hygiene and occupational pathology among office workers].

Northwest public health research center, Ministry of health and social affairs, St.-Petersburg. The article substantiates the conception of "office room", "office worker", estimates the basic diseases and symptoms among office workers (SBS-syndrome, BRI-illnesses, BRS-symptoms). Complex of indoor factors of office environment are analyzed, which influence the health status of personnel--indoor air quality (microclimate, aerosols, chemical, biological pollution, air ionization), external physical factors, ergonomics, intensity and tension of work, psychosocial factors. Comparison of Russian and foreign approaches to the hygienic estimation and rating of these factors was carried out. Owing to inadequacy of Russian hygienic rules to modern requirements, the necessity of working out of a complex of sanitary rules focused particularly on office workers is proved.

[Work schedule of electric welders in heating microclimate].

The authors present results of specified hygienically justified timing regulations for setting the variants of work and rest modes within electric welders shift, who work in various postures with variable visual strain while welding in heating microclimate.

[Investigating occupational risk in visual strain].

The article deals with materials of industrial and laboratory studies on risk of acute and chronic functional overstrain due to visual work in humans. The authors demonstrated value of intensity, structure and duration of visual strain as etiologic risk factors.

[The medical social characteristics of patients hospitalized in the traumatic surgery department].

The medical social characteristics of injured patients hospitalized in the traumatic surgery department of municipal hospital. The study results permitted to develop the organizational functional model of injury prevention management. The pattern presuppose the implementation of complex of activities considering the options of target population groups and measures realization period based on the regular occurrences revealed in the study.

[The management of emergency medical care hospital in force-majeure situation].

The development of the Moscow emergency medical care service depends on the highly social significance of the investigation of mass injuries under force majeure situations and lethality structure. The elaborated and approved algorithm of providing high-quality medical care under mass admission of force-majeure situation victims to the municipal emergency medical care hospital is discussed.

[The medical social characteristics of able-bodied population mortality as a result of traffic accidents].

The analysis of the medical social portrait of the deceased in consequence of traffic accidents was implemented. The risk groups and the pattern of mortality indicators distribution according the days of week and seasons were revealed. The major medical social factors of traffic accidents making resulted in fatal outcomes were established considering the characteristics of injuries due to traffic accidents demanding the organization of opportune medical care.

[Using pulse measurements for physiological regulation of work intensity]. / Ispol'zovanie pul'sometrii dlia fiziologicheskogo normirovaniia intensivnosti truda.

The article covers materials of physiologic evaluation of work intensity through pulse measurements. The authors stress drawbacks of present norms for average shift pulse rate. To evaluate work intensity, the researchers suggest normal value of pulse rate change at the 7th work hour vs at the 2nd hour.

[Regulation of work intensity according to duration, density and pace]. / Normirovanie napriazhennosti truda po ego prodolzhitel'nosti, plotnosti i tempu.

The author describes a method for hygienic regulation of work intensity. The parameters used are "average shift working pace" and "physiologic intensity of work" that summarize three time characteristics of occupational activities--working week duration, density of working time (presence of breaks and pauses within a shift), working pace.

[Tumor growth index as an integral criterion for the efficacy of antineoplastic therapy under experimental conditions]. / Indeks rosta opukholi kak integral'nyi kriterii éffektivnosti protivoopukholevoi terapii v éksperimente.

An additional criterion is suggested for description of effectiveness of therapy of experimental tumors--tumor growth index. It is a ratio of area covered by the kinetic curve of tumor growth in the study group and that in control. The index is intended to describe the efficacy of therapy in terms of degree and duration of the effect.

[The integral assessment of subject symptoms for detecting chronic visual, motor and nonspecific fatigue in workers]. / Integral'naia otsenka sub''ektivnykh simptomov dlia vyiavleniia khronicheskogo zritel'nogo, dvigatel'nogo i nespetsificheskogo pereutomleniia rabotaiushchikh.

The authors present methods of integral evaluation of symptoms for diagnosis of chronic visual, locomotor and nonspecific fatigue in workers. Questionnaires and algorithms to treat them are incorporated into the methods that are to be used in hygienic regulation of work, particularly, to reveal comparative significance of work factors--visual efforts, physical hardiness and nerve strain.