[Impact of neutron radiation on the viability of tumor cells cultured in the presence of boron-10 isotope].

Objective: To investigate the impact of a neutron beam formed with the accelerator-based epithermal neutron source designed at the G.I. Budker Institute of Nuclear Physics (INP) on the viability of human and animal tumor cells cultured in the presence of boron-10 isotope. Material and Methods: Human U251 and T98G glioma cells and Chinese hamster CHO-K1 and V-79 cells were incubated at various concentrations in the culture medium containing 10B-enriched L-boronophenylalanine. The cells were irradiated with a neuron beam using the accelerator-based epithermal neuron source. A clonogenic assay was used to evaluate the viability of the irradiated cells. The absorbed doses obtained from elastic scattering of fast neutrons by substance nuclei and the doses obtained from boron neutron capture were calculated using the NMS code. The absorbed doses of gamma-radiation were measured with a mixed radiation dosimeter. Results: The viability of boron-containing and intact human U251 and T98G cell lines and Chinese hamster CHO-K1 and V-79 cells was analyzed after neutron beam radiation. Irradiation of all four cell lines were cultured in the presence of 10B was shown to reduce their colony-forming capacity compared with the control. Elevated boron levels in the culture medium resulted in a significant decrease in the proportion of survived cells. Radiation had the most pronounced impact on the proliferative capacity of the human U251 glioma cell lines. Conclusion: The cultures of human tumor cells and mammalian cells demonstrated that the neutron beam formed with the accelerator-based epithermal neutron source designed at the INP, was effective in reducing the viability of tumor cells in the presence of 10B.

Effect of epithermal neutrons on viability of glioblastoma tumor cells in vitro.

We studied in vitro effect of epithermal neutrons in various doses on viability of glioblastoma U87 tumor cells. Increasing the dose from 1.9 to 4.1 Sv promoted cell death. Cytofluorimetric analysis revealed no activation of apoptosis in the irradiated cells, which attested to necrotic death of the tumor cells exposed to epithermal neutron radiation.

[The effect of direct x-ray contrast lymphography on pulmonary perfusion function]. / Vliianie priamoi rentgenokontrastnoi limfografii na sostoianie legochnoi perfuzii.

Overall twenty-five persons (8 males and 17 females) were enrolled in a study on the influence of straight radiopaque lymphography with an oily substance, on the condition of pulmonary microcirculation, as evidenced by perfusion pulmoscintigraphy. There was a significant decrease in the accumulation of 99m-Tc-macroaggregate (TCK-8) in lower portions of both lungs 24 hours after the lymphography and normal dispersal 10 days after the study made. The authors suggest that the radiopaque substance might stimulate the inflammatory processes in chronic nonspecific diseases of the lungs.

[Scintigraphy of the bone marrow using 111In citrin in the diagnosis of hematopoietic disorders following chemotherapy]. / Stsintigrafiia kostnogo mozga s 111In-tsitrinom v diagnostike narushenii gemopoéza posle khimioterapii.

Diagnostic potentialities of the use of a new radiopharmaceutical 111In-citrin were studied to assess the hemopoietic status on the basis of examination of 47 cancer patients after chemotherapy. Bone marrow function was assessed on the basis of the visual RP distribution in the bone marrow and a study of quantitative indices of the agent accumulation in various anatomical regions. The results of the study showed that a decrease in the agent accumulation in the flat pelvic bones was determined in patients with stable hemocytopenia. Hyperfixation of the agent was undetectable in the long tubular bones. Raised accumulation of the indicator in the metaepiphyses of the long tubular bones was observed in parallel with lowered accumulation of the agent in the flat bones in the group of patients without hemocytopenia nearly in half of the cases. The accumulation of RP in the rest of the cases did not differ from that in the control group. The results obtained showed that 111In-citrin accumulation in the bone marrow of the long tubular bones indicating its functional rearrangement and the normal level of the indicator accumulation in the main zones of bone marrow hemopoiesis in cancer patients could serve as a favorable prognostic sign for the assessment of hemopoiesis in subsequent courses of cytostatic therapy.

[Radionuclide lympho- and venography in the diagnosis of radiation edema of the lower extremities]. / Radionuklidnaia limfo- i venografiia v diagnostike luchevykh otekov nizhnikh konechnostei.

Lymphography and venography were used for diagnosis of radiation edemas of the lower limbs in patients receiving radiation or combined therapy for malignant tumors of the female genital organs. It was established that 99mTc-lymphocys could be used to detect lymphostasis, to determine the level and nature of the lymphatic trochlea. An additional study of the venous blood flow made it possible to determine the type of disorder of the lymph flow. Radionuclide methods of the investigation of the lymph flow and venous blood flow are easy to use, atraumatic and quite informative, therefore they should be recommended for the investigation of patients with radiation edemas of the limbs to determine indications for surgery.

[Scintigraphy of the bone marrow with 111In-citrin in polycythemia vera]. / Stsintigrafiia kostnogo mozga s 111In-tsitrinom pri istinnoi politsitemii.

Scintigraphy of the bone marrow with 111In-citrin was performed in 32 patients with polycythemia vera and 8 controls. The method ensured an objective assessment of bone marrow function in patients with polycythemia vera. A characteristic RP scintigraphic picture corresponded to different clinical stages of the disease. A degree of a decrease in the blood radioactivity within 24 h after i. v. administration of 111In-citrin reflected the summary erythropoietic activity of the bone marrow and could serve as a differential diagnostic criterion in determining a stage of polycythemia vera.