RESUMO
The location and level of IL-8 and TGF-beta2 expression in the fimbrial compartment of fallopian tubes and IL-10 expression in the endometrium of women with pyoinflammatory adnexal diseases were studied by in situ hybridization. These diseases are associated with considerable changes in the levels of local production of these cytokines. Inflammatory infiltration and epithelial cells were most active producers of IL-9 and TGF-beta2 in the fimbrial compartment of fallopian tubes, while in the endometrium IL-10 gene was expressed at a high level primarily in the glandular epithelial cells.
Assuntos
Doenças dos Anexos/imunologia , Endométrio/química , Tubas Uterinas/química , Interleucina-10/análise , Interleucina-8/análise , RNA Mensageiro/análise , Fator de Crescimento Transformador beta/análise , Adulto , Endométrio/citologia , Tubas Uterinas/anatomia & histologia , Feminino , Humanos , Hibridização In Situ , Inflamação/imunologia , Interleucina-10/genética , Interleucina-8/genética , RNA Mensageiro/genética , Fator de Crescimento Transformador beta/genética , Fator de Crescimento Transformador beta2RESUMO
In patients with pyoinflammatory adnexal diseases moderate or intense DNA degradation was observed in the majority of epithelial, stromal, and inflammatory infiltration cell in inflammatory foci in the fimbrial compartment of fallopian tubes without signs of tissue destruction. Expression of TNFR1 gene increased 2.7-fold and expression of Fas gene decreased 3.1-fold compared to intact endosalpinx, which indicates induction of apoptosis triggered by tumor necrosis factor-alpha and inhibition of the Fas-dependent pathway. No signs of apoptosis were detected in the endometrium. Generalized apoptosis in the fimbrial compartment of the tubes can be a mechanism limiting the inflammatory process.
Assuntos
Doenças dos Anexos/metabolismo , Apoptose/fisiologia , Endométrio/fisiologia , Tubas Uterinas/anatomia & histologia , Tubas Uterinas/fisiologia , Inflamação/metabolismo , Doenças dos Anexos/patologia , Adulto , Antígenos CD/genética , Antígenos CD/metabolismo , Endométrio/citologia , Endométrio/patologia , Tubas Uterinas/patologia , Feminino , Humanos , Marcação In Situ das Extremidades Cortadas , Inflamação/patologia , Receptores do Fator de Necrose Tumoral/genética , Receptores do Fator de Necrose Tumoral/metabolismo , Receptores Tipo I de Fatores de Necrose Tumoral , Fator de Necrose Tumoral alfa/metabolismo , Receptor fas/genética , Receptor fas/metabolismoRESUMO
Changes in the local expression of IL-1beta, IL-2, IL-4, IL-6, IL-8, IL-10, IL-12, IL-15, IL-18, TNF-alpha, IFN-gamma- and TGF-beta(2) genes in the uterine adnexa and endometrium were studied in women with pyoinflammatory adnexal diseases. Examination of tissue specimens from the uterine adnexa involved in inflammation revealed a direct correlation in the levels of mRNA production between IL-6 and IL-10 (r=0.93, p<0.1), IL-6 and IL-4 (r=0.96, p<0.01), IL-10 and IL-4 (r=0.91, p<0.01), IL-12 and IFN-gamma (r=0.98, p<0.01). Expression of IL-4 gene increased 5.1-fold (p=0.001), IL-6 2-fold (p=0.007), IL-8 90.2-fold (p=0.009), IL-10 2.9-fold (p=0.008), IL-12 2.3-fold (p=0.3), and TGF-beta(2) gene 10.3-fold (p=0.1). In the endometrium of women with pyoinflammatory adnexal diseases only IL-10 gene expression increased (15.6-fold, p=0.007).
Assuntos
Anexos Uterinos/fisiologia , Doenças dos Anexos/imunologia , Citocinas/genética , Endométrio/fisiologia , Inflamação/imunologia , Anexos Uterinos/imunologia , Doenças dos Anexos/genética , Adulto , Citocinas/imunologia , Endométrio/imunologia , Feminino , Humanos , Inflamação/genética , Reação em Cadeia da Polimerase Via Transcriptase ReversaRESUMO
Production of interleukin, interferon-gamma, and transforming growth beta(2)-factor mRNA in peripheral blood mononuclear cells was studied in women with pyoinflammatory diseases of adnexa uteri. Predominant expression of Th1 proinflammatory cytokine genes in patients was demonstrated. Production of interleukins 4 and 8 mRNA decreased 200- and 60-fold, respectively, in comparison with that in healthy women. One month and a half after extirpation of fallopian tubes the cytokine production in blood mononuclear cells shifted towards antiinflammatory cytokines, expression of interleukin-8 gene returned to normal, and expression of interleukin-4 gene increased.
