Importance of palynology in the taxonomy of Piptolepis Sch.Bip. (Asteraceae: Lychnophorinae), a genus endemic to Brazil.

Lychnophorinae comprises 19 genera and 117 species. Its representatives are found mostly in the rocky grasslands ("campos rupestres") of Minas Gerais, Bahia, and Goiás States, Brazil. This study presents a palynological investigation of 10 species belonging to the genus Piptolepis, endemic to the rocky grasslands of the Espinhaço Mountains, in Minas Gerais State. The plant material used in this investigation was obtained from specimens deposited in Brazilian herbaria. Pollen grains were processed by the acetolysis method, measured, described, and photomicrographed in light microscopy and scanning electron microscopy. Morphological observations revealed Piptolepis pollen to be large, prolate spheroidal in most species, 3-colporate, ectoapertures long, sexine subechinolophate or sublophate. Endoapertures vary from almost circular, lalongate or lalongate, with a median constriction observed only in three species. Three types of spines were identified: prostrate and disorganized, elongated and narrow, and conical, erect, apex acute in the other species. This first palynological study of Piptolepis species allowed the separation of representatives of the genus using pollen keys. The most significant characters were pollen shape, ornamentation, endoaperture, and presence of median constriction in the endoaperture. The findings presented here do not support the infrageneric classification of Piptolepis, as pollen characters were not unique to any section.

Mental health at the COVID-19 frontline: An assessment of distress, fear, and coping among staff and attendees at screening clinics of rural/regional settings of Victoria, Australia.

PURPOSE: Research examining psychological well-being associated with COVID-19 in rural/regional Australia is limited. This study aimed to assess the extent of psychological distress, fear of COVID-19, and coping strategies among the attendees in COVID-19 screening clinics at 2 rural Victorian settings. METHODS: A cross-sectional study was conducted during July 2020 to February 2021 inclusive. Participants were invited to fill in an online questionnaire. Kessler Psychological Distress Scale (K-10), Fear of COVID-19 Scale, and Brief Resilient Coping Scale were used to assess psychological distress, fear of COVID-19, and coping, respectively. FINDINGS: Among 702 total participants, 69% were females and mean age (±SD) was 49 (±15.8) years. One in 5 participants (156, 22%) experienced high to very high psychological distress, 1 in 10 (72, 10%) experienced high fear, and more than half (397, 57%) had medium to high resilient coping. Participants with mental health issues had higher distress (AOR 10.4, 95% CI: 6.25-17.2) and fear (2.56, 1.41-4.66). Higher distress was also associated with having comorbidities, increased smoking (5.71, 1.04-31.4), and alcohol drinking (2.03, 1.21-3.40). Higher fear was associated with negative financial impact, drinking alcohol (2.15, 1.06-4.37), and increased alcohol drinking. Medium to high resilient coping was associated with being ≥60 years old (1.84, 1.04-3.24) and completing Bachelor and above levels of education. CONCLUSION: People who had pre-existing mental health issues, comorbidities, smoked, and consumed alcohol were identified as high-risk groups for poorer psychological well-being in rural/regional Victoria. Specific interventions to support the mental well-being of these vulnerable populations, along with engaging health care providers, should be considered.

Cyclic changes in the level of the innate immune molecule, surfactant protein-a, and cytokines in vaginal fluid.

PROBLEM: Our knowledge of the innate host defenses in the vagina, a site where these defenses are essential to protecting the host upper reproductive tract from invasion by pathogens, is as yet rudimentary. Specifically, little is known about the pattern-recognition component of vaginal innate immunity, the relationship of pattern-recognition molecules to known cytokine levels, and the role of gonadal hormones in their regulation. METHOD OF STUDY: We measured levels of Surfactant Protein-A (SP-A), a prototypic innate pattern-recognition protein, in vaginal fluid (VF) and correlated them with levels of IL-1ß and IL-8, two cytokines known to be present in VF. Assays were carried out on VF collected over three consecutive cycles from ten healthy naturally cycling women who were sampled at three specific time points in the menstrual cycle. The three time points were chosen to enable correlation with distinct hormonal states. RESULTS: Both SP-A and cytokines levels were highest 5-6 days after menses (P < 0.05) and were significantly lower at ovulation and mid-luteal phase. CONCLUSION: Surfactant Protein-A, like other host defense molecules in the reproductive tract, appears to be regulated by gonadal hormones.