Caracterização inicial do extrato da glândula salivar de Ornithodoros brasiliensis (Acari: Argasidae) na coagulação sanguínea

Introduction: Ticks are obligate hematophagous ectoparasitic arthropods of great relevance to public health, being vectors of various diseases. Ornithodoros brasiliensis (Acari: Argasidae) are endemic to the mountainous region of Rio Grande do Sul, Brazil. Their salivary composition contains bioactive molecules capable of modulating the immune system and affecting the host's hemostasis with local and systemic effects. Objectives: To characterize the anticoagulant activity of the salivary gland extract (SGE) of the tick Ornithodoros brasiliensis on blood coagulation. Methods: The EGSs were obtained by macerating the ticks' salivary glands, generating 2 different extracts (EGS-1 and EGS-2), after which the amount of protein was estimated by absorbance at 280 nm. SDS-PAGE 12.5% was used to analyze the protein profile of the samples and the biological test was carried out on the L1 and L2 larvae of the nematode Caenorhabditis elegans. EGS-1 was filtered on Amicon according to molecular weight (30, 10 and 3kDa) and the fractions obtained were subjected to RP-HPLC chromatography (C18). The chromatography samples and EGS-1 in different concentrations were tested in the rotational thromboelastometry equipment (Rotem®) (0.2 to 1.03 μg/well), as well as in the FXa and thrombin inhibition tests using chromogenic substrates (S2765 and S2238, respectively) (0.40 to 0.207 μg/test), following protocols pre- established by the laboratory and the equipment manual. EGS-2 was submitted in different concentrations (6.5 to 52 ng/well) to the rotational thromboelastometry test, and the global coagulation tests were carried out in duplicate, Prothrombin Time (PT) and Activated Thromboplastin Time (aPTT), using the commercial kits, with different concentrations of ESG-2 (6.5 to 97.5 ng/test) and finally FXa and thrombin inhibition tests using chromogenic substrates (S2765 and s2238, respectively) at a concentration of 6.5 ng/test. Results: EGS-1 showed in the protein dosage (1.118 μg/ml) and EGS-2 (6.5 μg/ml). In the activity test, the EGSs showed temporary and reversible paralysis. EGS-2 showed a prolongation of the clotting time with incoagulobility from 52 ng/well in the rotational thromboelastometry test. In TP, inhibition was 28.5% at 97.5 ng/well and in TTPa 48.6% at 78 ng/well. Thrombin inhibition averaged 13.7% and factor Xa inhibition averaged 30%. In relation to the chromatographic fractions, the P4 fraction showed a prolongation of the coagulation time in the rotational thromboelastometry test. The P3 fraction had a 25.4% inhibition of thrombin formation. The P4 fraction inhibited Factor Xa by 36.9%. Conclusion: The EGS of the O. brasiliensis tick has anticoagulant action, reaching incoagulability with 52ng/test in the rotational thromboelastometry assay and at least two proteins acting in the process. One of them is linked to thrombin, with the P3 fraction showing the greatest inhibition, and the other is linked to FXa inhibition, with the P4 fraction being more evident. The rotational thromboelastometry test showed an alteration, indicating an inhibitory action on the formation of fibrin networks.

Introdução: Os carrapatos são artrópodes ectoparasitas hematófagos obrigatórios, de grande relevância para a saúde pública, sendo vetores de diversas doenças. Os Ornithodoros brasiliensis (Acari: Argasidae) são endêmicos da região serrana do Rio Grande do Sul, Brasil. Na sua composição salivar possui a presença de moléculas bioativas capazes de modular o sistema imunológico e afetar a hemostasia do hospedeiro com efeitos locais e sistêmicos. Objetivos: Caracterizar a atividade anticoagulante do extrato da glândula salivar (EGS) do carrapato Ornithodoros brasiliensis sobre a coagulação sanguínea Métodos: Os EGS’s foram obtidos por maceração das glândulas salivares dos carrapatos, gerando 2 extratos distintos (EGS-1 e EGS-2), em seguida, a quantidade de proteínas foi estimada por absorbância a 280 nm. O SDS-PAGE 12,5% foi usado para analisar o perfil proteico das amostras e o teste biológico foi realizado nas larvas L1 e L2 do nematoide Caenorhabditis elegans. O EGS-1 foi submetido à filtração em Amicon de acordo com peso molecular (30, 10 e 3kDa) e as frações obtidas foram submetidas a cromatografia RP-HPLC (C18). As amostras da cromatografia e o EGS-1 em diferentes concentrações foram testados no equipamento de tromboelastometria rotacional (Rotem®) (0,2 a 1,03 μg/poço), como também nos testes de inibição do FXa e trombina utilizando substratos cromogênicos (S2765 e S2238, respectivamente) (0,40 a 0,207 μg/teste), seguindo protocolos pré-estabelecidos pelo laboratório e o manual dos equipamentos. O EGS-2 foi submetido em diferentes concentrações (6,5 a 52 ng/poço) ao teste de tromboelastometria rotacional, sendo realizados os testes globais de coagulação em duplicada, Tempo de Protrombina (TP) e Tempo de Tromboplastina Activada (TTPa), utilizando os kits comerciais, com diferentes concentrações de ESG-2 (6,5 a 97,5 ng/teste) e por fim submetidos testes de inibição do FXa e trombina utilizando substratos cromogênicos (S2765 e s2238, respectivamente) em uma concentração de 6,5 ng/teste. Resultados: O EGS- 1 apresentou na dosagem proteica (1,118 μg/ml) e o EGS-2 (6,5 μg/ml). No teste de atividade os EGS’s apresentaram uma paralisia temporária e reversível. O EGS-2 apresentou um prolongamento no tempo de coagulação com incoagulobilidade a partir de 52 ng/poço no teste de tromboelastometria rotacional. No TP a inibição foi de 28,5% com 97,5 ng/poço e em TTPa de 48,6% com 78 ng/poço. Na inibição de trombina, teve uma média de inibição de 13,7% e na inibição de fator Xa uma média de 30%. Em relação às frações cromatográficas, a fração P4 apresentou um prolongamento no tempo de coagulação no teste de tromboelastometria rotacional. A fração P3 obteve uma inibição de 25,4% na formação de trombina. A fração P4 obteve uma inibição de 36,9% na inibição de Fator Xa. Conclusão: O EGS do carrapato O. brasiliensis apresenta ação anticoagulante chegando a incoagulabilidade com 52ng/teste no ensaio de tromboelastometria rotacional e pelo menos duas proteínas atuantes no processo. Uma delas ligada a trombina com a representação da fração P3 com maior inibição e outra ligada a inibição do FXa sendo mais evidente com a fração P4. No teste de Tromboelastometria rotacional mostrou uma alteração, apontando uma ação inibitória na formação das redes de fibrina.

The Clinical Relevance of Impulsivity in Substance Use Disorder Treatment: Examining Within-Treatment Changes and Relationship to Psychiatric Symptoms and Cravings in a Large Inpatient Sample.

OBJECTIVE: Impulsivity is a multidimensional construct consistently associated with problematic substance use, but less is known about its relevance to clinical outcomes. The current study examined whether impulsivity changed over the course of addiction treatment and whether the changes were associated with changes in other clinical outcomes. METHOD: Participants were patients in a large inpatient addiction medicine program (N = 817; 71.40% male). Impulsivity was assessed using a self-report measure of delay discounting (i.e., overvaluation of smaller immediate rewards) and the UPPS-P Impulsive Behavior Scale, a self-report measure of impulsive personality traits. Outcomes were psychiatric symptoms including depression, anxiety, posttraumatic stress disorder, and drug cravings. RESULTS: Within-subjects analyses of variance revealed significant within-treatment changes in all UPPS-P subscales, all psychiatric indicators, and craving (ps < .005), but not delay discounting. Changes in all UPPS-P traits, except for sensation seeking, were significantly positively associated with changes in psychiatric symptoms and cravings over the course of treatment (ps < .01). CONCLUSIONS: These findings reveal that facets of impulsive personality change across addiction treatment and are generally related to positive changes in other clinically relevant outcomes. Evidence of change despite no explicit intervention targeting suggests that impulsive personality traits may be viable treatment targets in substance use disorder treatment.

Prevalence of cannabis use disorder among individuals using medical cannabis at admission to inpatient treatment for substance use disorders.

INTRODUCTION: Cannabis is used for medical and recreational purposes and may result in cannabis use disorder (CUD). This study explored the prevalence of cannabis use disorder and other psychiatric comorbidities among inpatients undergoing treatment for substance use disorder who reported medical cannabis use at admission. METHODS: We assessed CUD and other substance use disorders based on DSM-5 symptoms, anxiety with the Generalized Anxiety Disorder scale (GAD-7), depression with the Patient Health Questionnaire (PHQ-9), and post-traumatic stress disorder with the PTSD Checklist for DSM-5 (PCL-5). We compared the prevalence of CUD and other psychiatric comorbidities between inpatients who endorsed the use of cannabis for medical purposes only vs those endorsing use for medical and recreational purposes. RESULTS: Among 125 inpatients, 42% reported medical use only, and 58% reported medical and recreational use (dual motives). For CUD, 28% of Medical-Only and 51% of Dual-Use motives patients met the diagnostic criteria for CUD (p = 0.016). High psychiatric comorbidities were present: 79% and 81% screened positive for an anxiety disorder, 60% and 61% screened positive for depression, and 66% and 57% screened positive for PTSD for the Medical-Only and Dual-Use inpatients, respectively. CONCLUSIONS: Many treatment-seeking individuals with substance use disorder who report medical cannabis use meet criteria for CUD, particularly those reporting concurrent recreational use.

Characterizing Clinical Heterogeneity in a Large Inpatient Addiction Treatment Sample: Confirmatory Latent Profile Analysis and Differential Levels of Craving and Impulsivity.

Background: Individuals with substance use disorders (SUDs) have highly heterogeneous presentations and identifying more homogeneous subgroups may foster more personalized treatment. This study used SUD and other psychiatric indicators to characterize latent subgroups of patients in a large inpatient addiction treatment program. The resulting subgroups were then analyzed with respect to differences on clinically informative motivational mechanisms. Methods: Patients (n = 803) were assessed for severity of SUD (ie, alcohol use disorder, drug use disorder), post-traumatic stress disorder, anxiety disorders, and major depressive disorder. Confirmatory latent profile analysis (CLPA) was used to identify latent subgroups, hypothesizing 4 subgroups. Subgroups were then characterized with respect to multiple indicators of impulsivity (ie, delay discounting and impulsive personality traits via the UPPS-P) and craving. Results: The CLPA confirmed the hypothesized 4-profile solution according to all indicators (eg, entropy = 0.90, all posterior probabilities ⩾.92). Profile 1 (n = 229 [32.2%], 24.9% female, median age in range of 45-49) reflected individuals with high alcohol severity and low psychiatric severity (HAlc/LPsy). Profile 2 (n = 193 [27.1%], 29.3% female, median age in range of 35-39) reflected individuals with high drug and psychiatric severity (HDrug/HPsy). Profile 3 (n = 160 [22.5%], 37.6% female, median age in range of 45-49) reflected individuals with high alcohol severity and psychiatric severity (HAlc/HPsy). Profile 4 (n = 130 [18.3%], 19.4% female, median age in range of 35-39) reflected individuals with high drug severity and low psychiatric severity (HDrug/LPsy). Both high comorbid psychiatric severity subgroups exhibited significantly higher craving and facets of impulsivity. Conclusions: The results provide further evidence of 4 latent subgroups among inpatients receiving addiction treatment, varying by alcohol versus other drugs and low versus high psychiatric comorbidity. Furthermore, they reveal the highest craving and impulsivity in the high psychiatric comorbidity groups, suggesting targets for more intensive clinical intervention in these patients.

Psychometric evaluation of the treatment entry questionnaire to assess extrinsic motivation for inpatient addiction treatment.

Introduction: Valid multi-faceted measurement of motivation for substance use disorder (SUD) treatment is needed to help inform treatment approaches and predict outcomes. This study examined evidence of validity for the Treatment Entry Questionnaire (TEQ-9). Methods: Data represented individuals entering inpatient SUD treatment (n = 1455). We used confirmatory factor analysis (CFA) to assess the three-factor structure of the TEQ-9 [identified (i.e., values/personally chooses treatment), introjected (i.e., internally controlled by guilt/shame) and external motivations (i.e., external pressure/demands)], and examined measurement invariance across gender, age, and ethno-racial identity. Correlation with readiness and confidence assessed convergent validity, while correlations with substance use problem severity and previous substance use treatment assessed meaningful group differences. Results: A three-factor structure was confirmed with all items loading significantly onto their respective factors (ps < 0.001). Each subscale demonstrated high internal consistency (Identified α = 0.90; Introjected α = 0.79; External α = 0.85). Each subscale demonstrated measurement invariance up to the scalar level across all sub-groups. Readiness, confidence, and substance use problem severity correlated as expected across various substances with the identified (rs = 0.098 - 0.262, ps < 0.05), and external (rs = -0.096 - -0.178, ps < 0.05) subscales. Additionally, the mean Identified subscale score was significantly higher among those who previously engaged in SUD treatment (p < 0.001). Findings for the Introjected subscale were more ambiguous. Conclusions: Findings provide evidence for factorial validity, measurement invariance, convergent validity and group differences of the TEQ-9 in a large clinically mixed inpatient SUD treatment population, providing further support of its clinical and research utility.

Using conventional and machine learning propensity score methods to examine the effectiveness of 12-step group involvement following inpatient addiction treatment.

BACKGROUND: Continuing care following inpatient addiction treatment is an important component in the continuum of clinical services. Mutual help, including 12-step groups like Alcoholics Anonymous, is often recommended as a form of continuing care. However, the effectiveness of 12-step groups is difficult to establish using observational studies due to the risks of selection bias (or confounding). OBJECTIVE: To address this limitation, we used both conventional and machine learning-based propensity score (PS) methods to examine the effectiveness of 12-step group involvement following inpatient treatment on substance use over a 12-month period. METHODS: Using data from the Recovery Journey Project - a longitudinal, observational study - we followed an inpatient sample over 12-months post-treatment to assess the effect of 12-step involvement on substance use at 12-months (n = 254). Specifically, PS models were constructed based on 34 unbalanced confounders and four PS-based methods were applied: matching, inverse probability weighting (IPW), doubly robust (DR) with matching, and DR with IPW. RESULTS: Each PS-based method minimized the potential of confounding from unbalanced variables and demonstrated a significant effect (p < 0.001) between high 12-step involvement (i.e., defined as having a home group; having a sponsor; attending at least one meeting per week; and, being involved in service work) and a reduced likelihood of using substances over the 12-month period (odds ratios 0.11 to 0.32). CONCLUSIONS: PS-based methods effectively reduced potential confounding influences and provided robust evidence of a significant effect. Nonetheless, results should be considered in light of the relatively high attrition rate, potentially limiting their generalizability.

Evaluating the Associations Between Exposure to Tobacco Interventions During Inpatient Treatment and Substance Use Outcomes: Findings From a Natural Experiment.

OBJECTIVE: In this study, we took advantage of a natural experiment that occurred within a substance use disorders (SUD) treatment setting which first saw the implementation of an evidence-based practice (EBP) for tobacco cessation, followed by the implementation of a tobacco-free policy (TFP) that included a campus-wide tobacco ban. We sought to examine how implementation of the EBP and TFP was associated with substances use outcomes, in addition to tobacco use, up to 3-months posttreatment. METHODS: Data were collected from patients in a substance use disorders treatment program at baseline, discharge, 1-, and 3-months posttreatment. Using a quasi-experimental design and generalized estimating equations, we modelled how patients' (N = 480) exposure to one of 3 interventions (1: treatment as usual [TAU], 2: EBP, and 3: EBP + TFP) was associated with overall abstinence from tobacco, alcohol, and other substances over time. Measures of tobacco use frequency, amount, and quit attempts were also modelled among a sub-sample of participants who self-reported using tobacco before treatment. RESULT: Exposure to the EBP + TFP was associated with increased tobacco abstinence (odds ratio [OR] = 1.93, 95% confidence interval [CI] [1.29, 2.90]) over time, including decreases in tobacco use frequency (OR = 0.78, 95% CI [0.68, 0.89]) and amount (OR = 0.80, 95% CI [0.67, 0.96]), and increased in likelihood of making a quit attempt (OR = 1.75, 95% CI [1.10, 2.80]) compared to TAU. Exposure was not associated with alcohol and/or other substance use. CONCLUSIONS: Comprehensive tobacco interventions that include EBP + TFP can promote tobacco cessation and reduced tobacco use following inpatient SUD treatment, without adversely affecting the use of other substances.

Predictors of premature treatment termination in a large residential addiction medicine program.

BACKGROUND: While inpatient programs are a common setting for addiction treatment, patients' premature termination is a major concern. Predicting premature treatment termination has the potential to substantially improve patient outcomes by identifying high-risk profiles and suggesting care paths that might reduce dropout. The current study examined the predictors of premature termination from an inpatient addiction medicine service. METHODS: In 1082 patients admitted to a large inpatient addiction medicine service, we used intake assessments of severity of alcohol use disorder, illicit drug use disorder, post-traumatic stress disorder (PTSD), anxiety disorders, and major depressive disorder to predict planned termination (n = 922) or premature termination (n = 160). We used two complementary analytic approaches-traditional binary logistic regression and a data-driven latent profile analysis (LPA). RESULTS: Binary logistic regression revealed that alcohol use severity, illicit drug use severity, and PTSD severity significantly predicted termination status, although alcohol use severity notably exhibited an inverse relationship. The LPA revealed four distinct profiles, with one profile exhibiting a significantly higher rate of premature termination and another exhibiting a significantly lower rate of premature termination. The high-risk profile was characterized by high drug severity, high comorbid psychopathology (PTSD, depression, and anxiety symptoms), but low alcohol severity. The low-risk profile was characterized by high alcohol severity, but low drug use and low comorbid psychopathology. CONCLUSIONS: These results provide converging evidence that illicit drug severity and psychiatric severity, and particularly PTSD, were associated with premature termination. Moreover, the LPA revealed distinct latent subgroups of patients with meaningfully higher and lower risk of premature termination, suggesting that addiction services should develop strategies for identifying high-risk individuals or develop care paths for high-risk symptom clusters. Approaches that are trauma-informed or otherwise focus on the management of comorbid psychiatric conditions may be particularly appropriate for reducing premature termination.

Evaluating the short-term impact of a tobacco-free policy in an inpatient addiction treatment setting.

PURPOSE: Tobacco use is highly prevalent within addiction treatment settings, despite the potential benefits that cessation may provide to patients' psychosocial functioning and overall sobriety. Moreover, tobacco cessation is often insufficiently addressed in addiction treatment programs, although evidence suggests that tobacco control policies, such as access to evidence-based treatment or tobacco-free policies, may be effective. The objective of our study was to evaluate the impact of the implementation of these two tobacco control policies in an inpatient addiction treatment centre. METHODS: Using a 3-group quasi-experimental design, we examined how the implementation of the Ottawa Model for Smoking Cessation (OMSC) and a subsequent campus-wide tobacco ban influenced patients' overall smoking status, frequency, amount used per day, and quit attempts during treatment, compared to usual care. Participants (N = 397) responded to a comprehensive questionnaire upon admission and discharge from an addiction treatment program. We used generalized linear mixed modelling to measure changes over time while accounting for relevant sociodemographic covariates. RESULTS: Patients exposed to a more comprehensive tobacco control environment (i.e., the OMSC, plus complete tobacco ban) were over 80% less likely to report having used tobacco during treatment, compared to patients exposed to usual care (AOR = 0.17, 95% CI [0.05-0.63]). Receiving treatment in this setting also contributed to a 35% decrease in the average number of days patients used tobacco compared to usual care (AOR = 0.65, 95% CI [0.53-0.98]), and a 27% decrease in the average number of cigarettes used per day compared to usual care (AOR = 0.73, 95% CI [0.58-0.93]). CONCLUSION: Comprehensive tobacco control policy interventions within inpatient addiction treatment hospitals promote tobacco cessation. Such interventions should include a combination of evidence-based treatment for patients and environmental restrictions to discourage tobacco use. The results of our study suggest that, within inpatient addiction treatment settings, use of the OMSC in combination with a campus-wide tobacco ban may be more effective than usual care or the OMSC alone.

Effects of 12-step mutual support and professional outpatient services on short-term substance use outcomes among adults who received inpatient treatment.

BACKGROUND: Continuing care is increasingly prioritized in the treatment of substance use disorders (SUDs). Ongoing engagement in continuing care, including mutual support (e.g., 12-step groups) and/or professional outpatient services, may enhance treatment outcomes and facilitate recovery. OBJECTIVE: This study investigates how engagement in 12-step mutual support and professional outpatient services is associated with short-term substance use outcomes in a sample of patients who completed inpatient SUDs treatment. METHODS: As part of the Recovery Journey Project - a longitudinal cohort study - participants completed questionnaires upon admission to an inpatient SUDs treatment program, and at 1- and/or 3-months post-discharge (n = 379). Baseline data were collected by self-administered, electronic questionnaires. Follow up data were collected by phone or email. Analyses involved multivariate Generalized Estimating Equations separately modelling self-reported abstinence and percent days abstinent (PDA) over the three time periods. RESULTS: Overall, rates of self-reported abstinence and PDA increased significantly from baseline to 1- and 3-months follow up. Engagement in 12-step activities (i.e., attended 30 meetings in 30 days, had a home group, had a sponsor, did service work) and professional outpatient substance use support were each significantly associated with abstinence and PDA. Participants who reported a higher degree of 12-step involvement (defined as engagement in more 12-step activities) were also more likely to report being abstinence and greater PDA. CONCLUSIONS: Engagement in continuing care, including 12-step activities and professional outpatient substance use support, was highly associated with substance use. Clinical teams should encourage participation in such activities to optimize treatment outcomes.

Evaluation of the effect of cafeteria diet on the kidney Na,K-ATPase activity, and oxidative stress.

In this study, renal tissue, subdivided into the cortex and medulla of Wistar rats subjected to a cafeteria diet (CAF) for 24 days or to normal diet, was used to analyze whether the renal enzyme Na,K-ATPase activity was modified by CAF diet, as well as to analyze the α1 subunit of renal Na,K-ATPase expression levels. The lipid profile of the renal plasma membrane and oxidative stress were verified. In the Na,K-ATPase activity evaluation, no alteration was found, but a significant decrease of 30% in the cortex was detected in the α1 subunit expression of the enzyme. There was a 24% decrease in phospholipids in the cortex of rats submitted to CAF, a 17% increase in cholesterol levels in the cortex, and a 23% decrease in the medulla. Lipid peroxidation was significantly increased in the groups submitted to CAF, both in the cortical region, 29%, and in the medulla, 35%. Also, a reduction of 45% in the glutathione levels was observed in the cortex and medulla with CAF. CAF showed a nearly two-fold increase in glutathione peroxidase (GPX) activity in relation to the control group in the cortex and a 59% increase in the GPx activity in the medulla. In conclusion, although the diet was administered for a short period of time, important results were found, especially those related to the lipid profile and oxidative stress, which may directly affect renal function.

Factors associated with a shorter duration of breastfeeding. / Fatores relacionados com uma menor duração total do aleitamento materno.

This article seeks to identify the factors associated with a longer duration of breastfeeding. A nested case-control study was carried out with a cohort of mothers for about two years after they gave birth to their children in the two largest public maternity hospitals in João Pessoa - PB. Mothers who breastfed up to 15 months were considered as cases (n = 55) and those who breastfed for more than 15 months (n = 48) were considered controls. The exposure variables were maternal socioeconomic characteristics, gestational characteristics, birth characteristics and early introduction of food. The Chi-Square test was applied to select the independent variables (p-value <= 0.20) to be entered into a multiple logistic regression model, with only those with a p-value <= 0.05 being kept in the final model. The early introduction of infant formula (OR = 4.71, CI95%: 1.76 - 12.63), other milks (OR = 3.25, CI95%: 1.27 - 8.31) and having less than six prenatal consultations (OR = 2.73, CI95%: 1.04 - 7.07) were risk factors for a shorter breastfeeding duration. The early introduction of infant formulas or other milks may be an important indicator for the adoption of appropriate breastfeeding promotion and support actions to achieve the WHO target of breastfeeding for two years or longer.

O objetivo deste artigo é identificar fatores relacionados com uma maior duração do aleitamento materno. Realizou-se um estudo caso-controle aninhado em uma coorte de mães que tiveram seus filhos nas duas maiores maternidades públicas de João Pessoa ­ PB quando eles tinham em torno de dois anos de idade. Os casos foram aquelas que amamentaram até o 15 mês (n = 55) e os controles as que amamentaram por mais de 15 meses (n = 48). As variáveis de exposição foram características socioeconômicas maternas, da gestação e parto e a introdução precoce de alimentação complementar. Aplicou-se o teste Qui-Quadrado para selecionar as variáveis independentes (p-valor <= 0,20) para ingressar em um modelo de regressão logística múltipla, permanecendo no modelo final somente aquelas com p-valor <= 0,05. A introdução precoce de fórmula infantil (OR = 4,71, IC95%: 1,76 ­ 12,63), de outros leites (OR = 3,25, IC95%: 1,27 ­ 8,31) e realizar menos de seis consultas pré-natal (OR = 2,73, IC95%: 1,04 ­ 7,07) foram fatores de risco para a menor duração do aleitamento materno. A introdução precoce de fórmulas infantis ou outros leites pode ser um indicador importante para a adoção de ações de promoção e apoio oportunas para o prolongamento da amamentação para atingir a meta da OMS de aleitamento materno por dois anos ou mais.

[Association between minor mental disorders and household feeding insecurity among mothers of children less than one-year-old]. / Associação entre risco de transtornos mentais comuns e insegurança alimentar entre mães com filhos menores de um ano de idade.

Household food insecurity (HFI) is related to health and nutritional problems, however there are few extant studies that relate it to mental health. The scope of this article is to associate HFI with the risk of minor mental disorders (MMD) in mothers with one-year-old children. A prospective cohort was conducted with 194 mothers and their babies from birth onwards. HFI was measured fourth months after birth using the Brazilian Household Food Insecurity scale and MMD risk amongst mothers was measured by the Self-Reporting Questionnaire (SRQ-20) when their baby was one year old. Socio-economic and health status were used for control. HFI was present in 59.3% of the families. The MMD risk ratio was 1.59 (CI 95%: 1.10 - 2.31) in HFI families compared with secure families. This relationship remains significant in the multiple logistic model, OR= 2.20 (CI 95%: 1.16 - 4.20), after controlling by socio-economic and health variables. HFI is an independent risk factor to maternal risk of MMD. It should be important to include strategies to reduce HFI by promoting maternal mental health and improving child-mother quality of life.

Insegurança alimentar e nutricional está relacionada a problemas nutricionais e de saúde, entretanto poucos estudos a relacionam com saúde mental. O objetivo deste artigo é Investigar associação da insegurança alimentar familiar e risco de transtornos mentais comuns (TMC) em mães com filhos de um ano de idade. Estudo de coorte prospectivo com 194 mães e seus filhos desde o nascimento. Insegurança alimentar foi medida aos quatro meses pós-parto, com a Escala Brasileira de Insegurança Alimentar e risco de diagnóstico positivo de TMC nas mães ao final do primeiro ano da criança, com o Self Response Questionnaire (SRQ-20). Medidas socioeconômicas e de saúde foram utilizadas como controle. Das famílias, 59,3% apresentavam insegurança alimentar, tendo razão de risco para diagnóstico de TMC de 1,59 (IC 95%: 1,10 ­ 2,31), comparado com famílias em segurança alimentar. Após ajuste, modelo logístico múltiplo estimou OR = 2,20 (IC 95%: 1,16 ­ 4,20) para esta relação. Observou-se associação da insegurança alimentar familiar aos quatro meses pós-parto e risco de diagnóstico de TMC entre mães ao final do primeiro ano dos filhos. O enfrentamento da insegurança alimentar deve fazer parte das estratégias promotoras da saúde materna e da qualidade de vida materno-infantil.

Fatores relacionados com uma menor duração total do aleitamento materno / Factors associated with a shorter duration of breastfeeding

Resumo O objetivo deste artigo é identificar fatores relacionados com uma maior duração do aleitamento materno. Realizou-se um estudo caso-controle aninhado em uma coorte de mães que tiveram seus filhos nas duas maiores maternidades públicas de João Pessoa - PB quando eles tinham em torno de dois anos de idade. Os casos foram aquelas que amamentaram até o 15 mês (n = 55) e os controles as que amamentaram por mais de 15 meses (n = 48). As variáveis de exposição foram características socioeconômicas maternas, da gestação e parto e a introdução precoce de alimentação complementar. Aplicou-se o teste Qui-Quadrado para selecionar as variáveis independentes (p-valor <= 0,20) para ingressar em um modelo de regressão logística múltipla, permanecendo no modelo final somente aquelas com p-valor <= 0,05. A introdução precoce de fórmula infantil (OR = 4,71, IC95%: 1,76 - 12,63), de outros leites (OR = 3,25, IC95%: 1,27 - 8,31) e realizar menos de seis consultas pré-natal (OR = 2,73, IC95%: 1,04 - 7,07) foram fatores de risco para a menor duração do aleitamento materno. A introdução precoce de fórmulas infantis ou outros leites pode ser um indicador importante para a adoção de ações de promoção e apoio oportunas para o prolongamento da amamentação para atingir a meta da OMS de aleitamento materno por dois anos ou mais.

Abstract This article seeks to identify the factors associated with a longer duration of breastfeeding. A nested case-control study was carried out with a cohort of mothers for about two years after they gave birth to their children in the two largest public maternity hospitals in João Pessoa - PB. Mothers who breastfed up to 15 months were considered as cases (n = 55) and those who breastfed for more than 15 months (n = 48) were considered controls. The exposure variables were maternal socioeconomic characteristics, gestational characteristics, birth characteristics and early introduction of food. The Chi-Square test was applied to select the independent variables (p-value <= 0.20) to be entered into a multiple logistic regression model, with only those with a p-value <= 0.05 being kept in the final model. The early introduction of infant formula (OR = 4.71, CI95%: 1.76 - 12.63), other milks (OR = 3.25, CI95%: 1.27 - 8.31) and having less than six prenatal consultations (OR = 2.73, CI95%: 1.04 - 7.07) were risk factors for a shorter breastfeeding duration. The early introduction of infant formulas or other milks may be an important indicator for the adoption of appropriate breastfeeding promotion and support actions to achieve the WHO target of breastfeeding for two years or longer.

Associação entre risco de transtornos mentais comuns e insegurança alimentar entre mães com filhos menores de um ano de idade / Association between minor mental disorders and household feeding insecurity among mothers of children less than one-year-old

Resumo Insegurança alimentar e nutricional está relacionada a problemas nutricionais e de saúde, entretanto poucos estudos a relacionam com saúde mental. O objetivo deste artigo é Investigar associação da insegurança alimentar familiar e risco de transtornos mentais comuns (TMC) em mães com filhos de um ano de idade. Estudo de coorte prospectivo com 194 mães e seus filhos desde o nascimento. Insegurança alimentar foi medida aos quatro meses pós-parto, com a Escala Brasileira de Insegurança Alimentar e risco de diagnóstico positivo de TMC nas mães ao final do primeiro ano da criança, com o Self Response Questionnaire (SRQ-20). Medidas socioeconômicas e de saúde foram utilizadas como controle. Das famílias, 59,3% apresentavam insegurança alimentar, tendo razão de risco para diagnóstico de TMC de 1,59 (IC 95%: 1,10 - 2,31), comparado com famílias em segurança alimentar. Após ajuste, modelo logístico múltiplo estimou OR = 2,20 (IC 95%: 1,16 - 4,20) para esta relação. Observou-se associação da insegurança alimentar familiar aos quatro meses pós-parto e risco de diagnóstico de TMC entre mães ao final do primeiro ano dos filhos. O enfrentamento da insegurança alimentar deve fazer parte das estratégias promotoras da saúde materna e da qualidade de vida materno-infantil.

Abstract Household food insecurity (HFI) is related to health and nutritional problems, however there are few extant studies that relate it to mental health. The scope of this article is to associate HFI with the risk of minor mental disorders (MMD) in mothers with one-year-old children. A prospective cohort was conducted with 194 mothers and their babies from birth onwards. HFI was measured fourth months after birth using the Brazilian Household Food Insecurity scale and MMD risk amongst mothers was measured by the Self-Reporting Questionnaire (SRQ-20) when their baby was one year old. Socio-economic and health status were used for control. HFI was present in 59.3% of the families. The MMD risk ratio was 1.59 (CI 95%: 1.10 - 2.31) in HFI families compared with secure families. This relationship remains significant in the multiple logistic model, OR= 2.20 (CI 95%: 1.16 - 4.20), after controlling by socio-economic and health variables. HFI is an independent risk factor to maternal risk of MMD. It should be important to include strategies to reduce HFI by promoting maternal mental health and improving child-mother quality of life.

A puérpera internada frente à prevenção da gravidez: possibilidades e limites da enfermagem / The puerperal woman hospitalized facing the prevention of pregnancy: possibilities and limits of nursing / El puerperal hospitalizada frente a la prevención del embarazo: posibilidades y límites de la enfermería

Objetivo: analisar a percepção da puérpera em relação ao planejamento reprodutivo. Método: estudo exploratório-descritivo, com abordagem qualitativa, realizada na Maternidade do Hospital Universitário Antônio Pedro/RJ. Foram entrevistadas 15 puérperas no Alojamento conjunto, por intermédio de entrevista semiestruturada, analisadas pela Técnica de Análise de conteúdo na modalidade temática. Resultados: na análise dos dados, emergiram duas categorias temáticas: << O planejamento reprodutivo vivenciado antes do período gravídico >>; << O planejamento reprodutivo idealizado após o parto >>. Conclusão: torna-se necessário a efetividade de ações com o intuito nos direitos sexuais e reprodutivos, com o foco no planejamento reprodutivo e nos direitos das mulheres, direitos esses conquistados pela luta do movimento feminista quanto ao controle de seu próprio corpo quanto às gestações.(AU)

Objective: to analyze the perceptions of puerperal women regarding reproductive planning. Method: descriptive exploratory study with a qualitative approach, carried out in the maternity ward of the University Hospital Antônio Pedro/RJ. We interviewed 15 mothers in the Rooming through semi-structured interviews and analyzed by content analysis technique in thematic modality. Results: in the data analysis, two thematic categories emerged: << Reproductive planning experienced before the pregnancy period >>; << The reproductive planning conceived after delivery >>. Conclusion: it is necessary to the effectiveness of actions in sexual and reproductive rights, with a focus on reproductive planning and women's rights, these rights won by the struggle of the feminist movement and the control of body as pregnancies.(AU)

Objetivo: analizar las percepciones de las puérperas con respecto a la planificación reproductiva. Método: estudio exploratorio descriptivo con un enfoque cualitativo, llevado a cabo en la sala de maternidad del Hospital Universitario Antonio Pedro/RJ. Entrevistamos a 15 madres en el alojamiento conjunto a través de entrevistas semiestructuradas y se analizaron mediante la técnica de análisis de contenido, modalidad temática. Resultados: en el análisis de datos, surgieron dos categorías temáticas: << planeación en la reproducción experimentó antes de que el período de embarazo >>; << La planificación reproductiva >> concebida después de la entrega. Conclusión: es necesario para la eficacia de las acciones en el orden en los derechos sexuales y reproductivos, con un enfoque en la planificación y los derechos reproductivos de la mujer, estos derechos conquistados por la lucha del movimiento feminista y el control de su propio cuerpo como embarazos.(AU)

Does Resuscitation Training Reduce Neonatal Deaths in Low-Resource Communities? A Systematic Review of the Literature.

Every year, nearly 1 million babies succumb to birth asphyxia (BA) within the Asia-Pacific region. The present study sought to determine whether educational interventions containing some element of resuscitation training would decrease the relative risk (RR) of neonatal mortality attributable to BA in low-resource communities. We systematically reviewed 3 electronic databases and identified 14 relevant reports. For community deliveries, providing traditional birth attendants (TBAs) with neonatal resuscitation training modestly reduced the RR in 3 of 4 studies. For institutional deliveries, training a range of clinical staff clearly reduced the RR within 2 of 8 studies. When resuscitation-specific training was directed to community and institutional health care workers, a slight benefit was observed in 1 of 2 studies. Specific training in neonatal resuscitation appears most effective when provided to TBAs (specifically, those presented with ongoing opportunities to review and update their skills), but this particular intervention alone may not appreciably reduce mortality.

Sexuality in later life: examining beliefs and perceptions of undergraduate students.

Understanding students' beliefs and perceptions of sex/sexuality in later life can reduce and prevent ageist myths and stereotypes. The objective of this study was to gauge undergraduate students' knowledge of several myths, stereotypes, and facts regarding sex/sexuality in later life, including lesbian, gay, bisexual, and transgendered (LGBT) older adults. More than 85% of students held a positive view of sex/sexuality in later life with 65% believing that they would engage in sexual activity past age 80 (N=125). Correct responses to true/false questions were higher for those with a positive perspective on aging, and recognizing that sexual behavior does not cease to be important with aging was the strongest predictor of holding a positive view on sexuality in later life. No significant differences were observed from responses regarding LGBT older adults or constraints to sexuality in long term care facilities. The positive perceptions among students in the current study suggest an increased acceptance of sexuality and diversity that should be maintained in university curricula.

Uma perspectiva mundial do carcinoma de células escamosas de pele / A world perspective of the squamous cell carcinoma of the skin

O Carcinoma de Células Escamosas (SCC) é a segunda neoplasia maligna cutânea mais freqüente, causada primordialmente pela exposição crônica e excessiva à radiação ultravioleta. O objetivo deste trabalho é discutir o perfil epidemiológico global do SCC através de uma revisão sistemática. Foi realizada uma busca na base de dados PUBMED com a utilização dos termos: squamous cells skin cancer, epidemiology squamous cells skin cancer, penile squamous cell carcinoma, squamous cell carcinoma of the penis, vulva SCC epidemiology e depletion of ozone layer. Outra busca foi realizada na base de dados SCIENCE DIRECT, com os termos: epidemiology of non-melanoma skin cancer, epidemiology of squamous cells carcinoma, squamous cells carcinoma, skin cancer, e, por fim, o termo UV radiation. No total, 19 artigos foram selecionados, sendo considerados aqueles publicados a partir de 2000. A revisão permitiu verificar que estudos em países de todos os continentes mostram um número crescente de casos, o que configura um grave problema de saúde pública mundial.