Recovery and encapsulation of Dunaliella salina ß-carotene through a novel sustainable approach: Sequential application of an ionic liquid as naturally-derived solvent and emulsifier.

Dunaliella salina is a promising source of ß-carotene, widely employed in the food industry. This study aimed to evaluate the sequential application of the Ionic Liquid (IL) cholinium oleate as an extraction solvent for D. salina ß-carotene recovery and, sequentially, as emulsifier for emulsion-based products obtained therefrom. The IL was evaluated regarding its ability to permeabilize the cells and recover ß-carotene at different temperatures (25-65 °C) and IL concentrations (0-46%). The use of the IL as solvent greatly improved ß-carotene recovery (>84%). The IL already present in the obtained extracts loaded with recovered ß-carotene was sequentially used as emulsifier in the production of nanoemulsions (NE). NE presented a ß-carotene entrapment efficiency of 100% and were kinetically stable for 30 days and presented droplet size, size distribution, and ζ-potential of 220 nm, 0.21, and -67 mV, respectively. These results indicate that using IL sequential as solvent and emulsifier has potential applications in the food industry.

Age-Related Variations in Breast Cancer Complications: Insights from Physical Medicine and Rehabilitation Clinical Practice.

Background Breast cancer patients often experience complications related to the disease or its treatment. With the rising average life expectancy, age is becoming less of a factor in treatment decisions for this condition. This study aims to evaluate differences in breast cancer complications among various age groups in patients referred to a hospital's physical medicine and rehabilitation (PMR) department. Methodology A retrospective study was conducted among all breast cancer patients evaluated in a PMR department between November 2019 and March 2021. Data were collected from patients' clinical files. SPSS® version 24 (IBM Corp., Armonk, NY, USA) was used for data analysis. Results We assessed 85 patients with a mean age of 56 years, finding that shoulder pain was the primary referral reason, reported by 85.9% of patients, followed by lymphedema in 32% of cases. Patients aged 56-65 years exhibited greater deficits in shoulder mobility compared to those between 66 and 75 years old, as well as greater functional limitations compared to other age groups. Most patients reported symptoms post-surgery, with an average delay of 24 months in PMR consultation. Despite this, nearly all patients (89.3%) reported clinical improvement following interventions. Conclusions We found that individuals in the 56-65-year age group were more prone to develop functional and shoulder mobility limitations. Despite delayed consultation, the majority of patients experienced clinical improvement, highlighting the intervention effectiveness of PMR interventions. These findings suggest that age alone may not be a determining factor in the reported breast cancer sequelae, implying the influence of other contributing factors in patient management. Further research is needed to elucidate the underlying mechanisms contributing to the diverse burden of disease sequelae observed across different age groups and to devise tailored interventions.

Managing carbon waste in a decarbonized industry: Assessing the potential of concrete mixing storage.

The effort towards a greener future will entail a shift to more environmentally friendly alternatives of many human activities. Within this context, the path towards a decarbonized society in general, and industrial decarbonization in particular, will require using low carbon solutions and/or capturing carbon emissions at the source. This flux of captured carbon will then require management and one option is to store it in concrete. The incorporation of the captured CO2 can be done during the mixing and/or curing. While the latter is more efficient and effective in terms of the amount of CO2 incorporated, it is limited to concrete in elements that are compatible with chamber curing. In practice, this would be restricted to the concrete pre-fabrication industry and, most probably, only to small size elements. Despite the lower performance, incorporation of CO2 into concrete during the mixing stage is a relatively universal alternative. The present research effort reveals that the latter solution is beneficial from an environmental point of view, with an estimated yearly carbon storage of 23 million tonnes worldwide against emissions of 2.5 million tonnes to do it.

Fuel consumption rate and emissions variability in waste collection services routes: case study of Cascais Ambiente.

In the design waste collection systems, it is common practice to use a constant specific fuel consumption (e.g. litres per amount of waste collected or distance travelled). This is also the approach used in many cases for fleet management, namely, for decision-making on more fuel-efficient equipment acquisition. However, the specific fuel consumption is not constant and there are spatial and temporal variations. Accounting for this variability becomes relevant if a more refined cost or environmental optimization is intended. The present research effort evaluates the energy intensity of the waste collection service in the Cascais municipality, reporting the differences and the magnitude of the variability for the mixed waste collection service. Statistically significant differences are found between the circuits, the trucks, months of the year and days of the week. It is discussed that extrapolating average fuel consumption rates for decision-making in new waste collection systems may be prone to substantial error.

Microalgae biomass as an alternative source of biocompounds: New insights and future perspectives of extraction methodologies.

Microalgae have characteristics that make them unique and full of potential. Their capacity to generate interesting bioactive molecules can add value to various industrial applications. However, most of these valuable compounds are intracellular, which makes their extraction a major bottleneck. Conventional extraction methodologies have some drawbacks, such as low eco-friendly character, high costs and energy demand, long treatment times, low selectivity and reduced extraction yields, as well as degradation of extracted compounds. The gaps found for these methods demonstrate that emergent approaches, such as ohmic heating, pulsed electric fields, ionic liquids, deep eutectic solvents, or high-pressure processing, show potential to overcome the current drawbacks in the release and extraction of added-value compounds from microalgae. These new processing techniques can potentially extract a variety of compounds, making the process more profitable and applicable to large scales. This review provides an overview of the most important and promising factors to consider in the extraction methodologies applied to microalgae. Additionally, it delivers broad knowledge of the present impact of these methods on biomass and its compounds, raising the possibility of applying them in an integrated manner within a biorefinery concept.

Personalizable AI platform for universal access to research and diagnosis in digital pathology.

BACKGROUND AND MOTIVATION: Digital pathology has been evolving over the last years, proposing significant workflow advantages that have fostered its adoption in professional environments. Patient clinical and image data are readily available in remote data banks that can be consumed efficiently over standard communication technologies. The appearance of new imaging techniques and advanced artificial intelligence algorithms has significantly reduced the burden on medical professionals by speeding up the screening process. Despite these advancements, the usage of digital pathology in professional environments has been slowed down by poor interoperability between services resulting from a lack of standard interfaces and integrative solutions. This work addresses this issue by proposing a cloud-based digital pathology platform built on standard and open interfaces. METHODS: The work proposes and describes a vendor-neutral platform that provides interfaces for managing digital slides, and medical reports, and integrating digital image analysis services compatible with existing standards. The solution integrates the open-source plugin-based Dicoogle PACS for interoperability and extensibility, which grants the proposed solution great feature customization. RESULTS: The solution was developed in collaboration with iPATH research project partners, including the validation by medical pathologists. The result is a pure Web collaborative framework that supports both research and production environments. A total of 566 digital slides from different pathologies were successfully uploaded to the platform. Using the integration interfaces, a mitosis detection algorithm was successfully installed into the platform, and it was trained with 2400 annotations collected from breast carcinoma images. CONCLUSION: Interoperability is a key factor when discussing digital pathology solutions, as it facilitates their integration into existing institutions' information systems. Moreover, it improves data sharing and integration of third-party services such as image analysis services, which have become relevant in today's digital pathology workflow. The proposed solution fully embraces the DICOM standard for digital pathology, presenting an interoperable cloud-based solution that provides great feature customization thanks to its extensible architecture.

Integrating Pool-seq uncertainties into demographic inference.

Next-generation sequencing of pooled samples (Pool-seq) is a popular method to assess genome-wide diversity patterns in natural and experimental populations. However, Pool-seq is associated with specific sources of noise, such as unequal individual contributions. Consequently, using Pool-seq for the reconstruction of evolutionary history has remained underexplored. Here we describe a novel Approximate Bayesian Computation (ABC) method to infer demographic history, explicitly modelling Pool-seq sources of error. By jointly modelling Pool-seq data, demographic history and the effects of selection due to barrier loci, we obtain estimates of demographic history parameters accounting for technical errors associated with Pool-seq. Our ABC approach is computationally efficient as it relies on simulating subsets of loci (rather than the whole-genome) and on using relative summary statistics and relative model parameters. Our simulation study results indicate Pool-seq data allows distinction between general scenarios of ecotype formation (single versus parallel origin) and to infer relevant demographic parameters (e.g. effective sizes and split times). We exemplify the application of our method to Pool-seq data from the rocky-shore gastropod Littorina saxatilis, sampled on a narrow geographical scale at two Swedish locations where two ecotypes (Wave and Crab) are found. Our model choice and parameter estimates show that ecotypes formed before colonization of the two locations (i.e. single origin) and are maintained despite gene flow. These results indicate that demographic modelling and inference can be successful based on pool-sequencing using ABC, contributing to the development of suitable null models that allow for a better understanding of the genetic basis of divergent adaptation.

Differences in Trapezius Muscle H-Reflex between Asymptomatic Subjects and Symptomatic Shoulder Pain Subjects.

In chronic shoulder pain, adaptations in the nervous system such as in motoneuron excitability, could contribute to impairments in scapular muscles, perpetuation and recurrence of pain and reduced improvements during rehabilitation. The present cross-sectional study aims to compare trapezius neural excitability between symptomatic and asymptomatic subjects. In 12 participants with chronic shoulder pain (symptomatic group) and 12 without shoulder pain (asymptomatic group), the H reflex was evoked in all trapezius muscle parts, through C3/4 nerve stimulation, and the M-wave through accessory nerve stimulation. The current intensity to evoke the maximum H reflex, the latency and the maximum peak-to-peak amplitude of both the H reflex and M-wave, as well as the ratio between these two variables, were calculated. The percentage of responses was considered. Overall, M-waves were elicited in most participants, while the H reflex was elicited only in 58-75% or in 42-58% of the asymptomatic and symptomatic participants, respectively. A comparison between groups revealed that the symptomatic group presented a smaller maximum H reflex as a percentage of M-wave from upper trapezius and longer maximal H reflex latency from the lower trapezius (p < 0.05). Subjects with chronic shoulder pain present changes in trapezius H reflex parameters, highlighting the need to consider trapezius neuromuscular control in these individuals' rehabilitation.

Combining green roofs and rainwater harvesting systems in university buildings under different climate conditions.

Rainwater Harvesting Systems (RWHS) are an alternative solution with the potential to increase water supply security and reduce the pressure on water resources and urban stormwater drainage systems. Likewise, Green Roofs are a nature-based solution with several ecosystem services able to improve well-being in densely urbanized areas. Despite these benefits, the combination of both solutions is still a knowledge gap to be explored. To address this issue, the paper explores the potential of integrating traditional rainwater harvesting systems (RWHS) with extensive green roofs (EGR), while simultaneously evaluating the performance of traditional RWHS in buildings with high and variable water consumption patterns across different climatic conditions. The analyses were carried out assuming two university buildings hypothetically located in three distinct climates (Aw - Tropical Savanna, Cfa - Humid Subtropical, and Csa - Hot-summer Mediterranean). The results show that the relationship between available water and demand is the key factor that defines if the system is most suitable for saving water, reducing stormwater runoff rates, or dual-purpose (when there is a balance between non-potable water supply and capture of stormwater). Combined systems were most effective when there is a balanced rainfall distribution over the year, as in humid subtropical regions. Under these conditions, a combined system designed for dual purposes could potentially have a green roof coverage of up to 70% of the total catchment area. Conversely, climates that have well-defined rainy and dry seasons such as Aw and Csa, may limit the effectiveness of a combined system (RWHS+EGR), being not able to supply water demands during certain periods of the year. However, if the primary objective is effective stormwater management, a combined system should be strongly considered. Because green roofs provide other ecosystem benefits that can contribute to enhancing urban resilience in the face of climate change.

Study on the Micro-Abrasion Wear Behavior of PVD Hard Coating under Different SiC Abrasive Particles/Distilled Water Ratios.

Microscale abrasion has been intensively used to study the wear behavior o several hard coatings, enabling the observation of different wear mechanisms. Recently, a study arguing whether the surface texture of the ball could influence the dynamics of abrasive particles throughout the contact was presented. In this work, the influence of the abrasive particles concentration able to change the texture of the ball was studied to understand its influence on the wear modes-rolling or grooving. Thus, tests were carried out using samples with a thin coating of TiN, deposited using the Physical Vapor Deposition (PVD) technique, and AISI 52100 steel balls etched over 60 s to induce a change in their texture and roughness were used. Three abrasive slurries were prepared with black silicon carbide (SiC) particles (average particle size of 4 µm) with different concentrations, 0.25, 0.35, and 0.45 g/cm3. The rotation speed used in the tests was 80 rpm and the normal loads applied in the study were 0.2 N and 0.5 N, and 1 N. After the wear tests, the coated samples and tracks on the surface of the balls were observed by SEM and 3D microscopy to understand the abrasive particle dynamics, evaluating the wear mode transition as well as the function of both applied load and slurry concentration. The tracks in the balls showed particles embedded on their surface. A lower concentration of abrasion was conducted to higher specific wear rate. Moreover, a predominant two-body wear mechanism was induced when the abrasive concentration was increased. There was an increase in the roughness of the scar and the surface of the balls with an increase in the abrasive particles' concentration.

Study of the Chlorine Influence on the Corrosion of Three Steels to Be Used in Water Treatment Municipal Facilities.

Many municipal facilities, such as pools and drinking water treatment facilities, are subject to ongoing maintenance due to the corrosion of their metallic materials caused by chlorine, leading to high costs and a possible risk to public health. A proper study of the employed product's effect could lead to the use of better materials, which significantly increase the lifetime of metallic equipment more attacked by corrosion, through studies evaluating their cost-effectiveness. This paper was carried out with the objective of studying the degradation of some metallic materials (AISI 316L, AISI 321 and Duplex 14462) used in the referred facilities in order to select the one that possessed a better behavior. It was observed that the introduction of some more adequate materials can drastically reduce maintenance operations, with Duplex 14462 showing the best results, ideal for greater chlorine concentrations, followed by AISI 321, which may be employed for components in less contact with chlorine, since it is more easily affordable.

The Impact of the Deterioration on Wood by Chlorine: An Experimental Study.

The use of disinfection and cleaning chemicals in several municipal facilities, such as swimming pools and drinking water treatment plants, causes the degradation of various types of wood, which leads to failures in equipment and the corresponding need for maintenance. This degradation creates added costs for municipalities, as well as the closure of certain facilities due to curative or preventive maintenance and, in many cases, public health issues, due to the water being contaminated with deteriorating products. Through a thorough study of the degradation effect on the products, more resistant materials can be found which are able to withstand these adversities and increase the lifespan of wood in regular contact with chemical agents. This is achievable by the determination of the cost-effectiveness of the substitute material to replace these components with alternative ones, with properties that better resist the deterioration effects promoted by aggressive environments. No studies have been found so far strictly focused on this matter. The objective of this study is to evaluate the degradation presented by two types of wood, beech and oak, which are exposed to the action of chlorine in municipal facilities. This degradation varies according to the chlorine content and the materials' time of contact with the chemical agent, allowing the selection of new materials which will provide an extended lifetime of the components, reducing maintenance drastically, as well as costs for the facilities and the risk to public health. The performed experimental tests have shown that the oak wood has the best results regarding chlorine degradation resistance.

Potential of rainwater harvesting in the retail sector: a case study in Portugal.

Water is a crucial resource for life, and it is increasingly scarce in many regions of the globe. In addition, retail water use is responsible for up to 19% of public water globally supplied. Hence, this study has set out to explore the technical and economic feasibility of rainwater harvesting systems as an alternative water source for a retail store located in southern Portugal. Water consumption data from 2018 to 2021 was collected from water bills, placing average monthly water consumption at around 400 m3. Next, rainfall data was collected from the nearest meteorological station, comprising 54 years of daily rainfall data between 1932 and 2008 with an annual average of 685 mm. The simulation of a rainwater harvesting system was performed, resorting to the mass-balance model. The optimal tank size was found to be 100 m3 considering simply the relation with the relative water savings variation on the graph relating the water savings with the tank size. Results show that the simulated rainwater harvesting system would allow saving 32-36% of the water consumed, despite the store's location in a dry climate, representing a financial gain of €330-372 per month. Findings suggest a substantial potential for the technical and economic feasibility of rainwater systems in retail stores, which makes them relevant solutions to achieve important water-savings in the retail sector, thus positively influencing retailers' direct water footprint.

Industrial production of recycled cement: energy consumption and carbon dioxide emission estimation.

The urge to reduce greenhouse gas emissions, in particular carbon dioxide, is a global problem, not only in spatial terms but also in terms of the scope of activities and sectors involved. Nevertheless, some sectors/industries are more critical due to their overall contribution to the problem, which is the case of the Portland cement industry. The present research estimates the energy consumption and carbon emissions associated with a novel process for producing cement by recycling used concrete and mortars. The novel process assessed resorts to the magnetic separation of the cement paste from the aggregates, followed by the thermal reactivation of the cement paste. Comparing the recycled cement production with the clinker production, higher energy consumption (over 9000 MJ/t compared with roughly 4000 MJ/t for Portland cement) and lower carbon dioxide emissions (average 730 kg CO2/t compared with more than 800 kg CO2/t for Portland cement) were estimated. However, the potential benefits in an industrial application are potentially much higher with the optimization of the production process. In particular, improvements in the washing and drying of the material prior to the magnetic separation will be critical since most of the energy is consumed in the process of drying.

Rapid and predictable genome evolution across three hybrid ant populations.

Hybridization is frequent in the wild but it is unclear when admixture events lead to predictable outcomes and if so, at what timescale. We show that selection led to correlated sorting of genetic variation rapidly after admixture in 3 hybrid Formica aquilonia × F. polyctena ant populations. Removal of ancestry from the species with the lowest effective population size happened in all populations, consistent with purging of deleterious load. This process was modulated by recombination rate variation and the density of functional sites. Moreover, haplotypes with signatures of positive selection in either species were more likely to fix in hybrids. These mechanisms led to mosaic genomes with comparable ancestry proportions. Our work demonstrates predictable evolution over short timescales after admixture in nature.

Life Cycle Assessment of Thermoactivated Recycled Cement Production.

The urgent need to tackle the effects of global warming has led to a worldwide compromise and ever-more demanding regulations. In this respect, as an important greenhouse gas emitter, the cement industry has to implement major changes in its production processes to achieve future goals. In this perspective, low-carbon eco-efficient cement, such as the thermoactivated recycled cement from concrete waste (RCC), seem to be a promising alternative to current carbon-intensive binders, such as ordinary Portland cement (OPC). This study aimed to demonstrate the potential contribution of RCC to the reduction in the environmental impacts of the cement industry, by means of a comparative life cycle assessment of three production methods of this binder (wet (WM), dry (DM) and air clean (ACM) methods) and OPC. Overall, RCC WM did not turn out to be a good alternative to OPC, essentially owing to the amount of fuel and electricity required for washing and drying the particles before the magnetic separation. On the other hand, RCC DM and RCC ACM proved to be promising alternatives to RCC WM and OPC, with a relevant reduction in all impact categories.

Intentional Replantation as a Starting Approach for a Multidisciplinary Treatment of a Mandibular Second Molar: A Case Report.

Intentional replantation (IR) may offer a solution for persistent periapical lesions associated with endodontically treated teeth. A 35-year-old male patient presented with pain associated with the left mandibular second molar and hypoesthesia. Upon clinical examination, increased probing pocket depth in the mid-buccal surface was detected. Cone beam computed tomography revealed a previous non-surgical root canal treatment, with root canal filling material extrusion adjacent to the inferior alveolar nerve, a fractured instrument in the mesial root, and a large periapical radiolucency involving both teeth 37 and 36. A diagnosis of symptomatic post-treatment apical periodontitis was established. After discussing treatment options with the patient, an IR of tooth 37 was performed. Extra-oral procedures were completed in 17 min. At 9 months, hypoesthesia resolution was reported, and apical healing was radiographically observed. After 2.5 years, the replanted tooth showed extensive root resorption. An extraction with alveolar ridge preservation, using leukocyte-platelet rich fibrin (L-PRF), was performed. Six months after tooth extraction and regeneration, implant placement surgery was carried out. IR presents a valid treatment modality for the management of post-treatment apical periodontitis. When orthograde retreatment or apical microsurgery prove to be unfeasible, IR is a unique procedure with the potential to promote tooth preservation in properly selected cases. Although unsuccessful after 2.5 years, the IR of tooth 37 allowed for bone regeneration, the maintenance of tooth 36 vitality, and hypoesthesia resolution.

Whole-genome analysis of multiple wood ant population pairs supports similar speciation histories, but different degrees of gene flow, across their European ranges.

The application of demographic history modelling and inference to the study of divergence between species has become a cornerstone of speciation genomics. Speciation histories are usually reconstructed by analysing single populations from each species, assuming that the inferred population history represents the actual speciation history. However, this assumption may not be met when species diverge with gene flow, for example, when secondary contact may be confined to specific geographic regions. Here, we tested whether divergence histories inferred from heterospecific populations may vary depending on their geographic locations, using the two wood ant species Formica polyctena and F. aquilonia. We performed whole-genome resequencing of 20 individuals sampled in multiple locations across the European ranges of both species. Then, we reconstructed the histories of distinct heterospecific population pairs using a coalescent-based approach. Our analyses always supported a scenario of divergence with gene flow, suggesting that divergence started in the Pleistocene (c. 500 kya) and occurred with continuous asymmetrical gene flow from F. aquilonia to F. polyctena until a recent time, when migration became negligible (2-19 kya). However, we found support for contemporary gene flow in a sympatric pair from Finland, where the species hybridise, but no signature of recent bidirectional gene flow elsewhere. Overall, our results suggest that divergence histories reconstructed from a few individuals may be applicable at the species level. Nonetheless, the geographical context of populations chosen to represent their species should be taken into account, as it may affect estimates of migration rates between species when gene flow is spatially heterogeneous.