Batch injection analysis towards auxiliary diagnosis of periodontal diseases based on indirect amperometric detection of salivary α-amylase on a cupric oxide electrode.

This study describes, for the first time, the use of a batch injection analysis system with amperometric detection (BIA-AD) to indirectly determine salivary α-amylase (sAA) levels in saliva samples for chronic periodontitis diagnosis. A chemical/thermal treatment was explored to generate a CuO film on a Cu electrode surface. This procedure offered good stability (RSD = 0.3%), good repeatability (RSD < 1.3%) and excellent reproducibility (RSD < 1.5%). The sAA concentration levels were determined based on the detection of maltose produced by enzymatic hydrolysis of starch. The analytical performance was investigated, and a linear correlation was observed for a maltose concentration range between 0.5 and 6.0 mmol L-1 with a correlation coefficient equal to 0.999. The analytical sensitivity and the limit of detection were 48.8 µA/(mmol L-1) and 0.05 mmol L-1, respectively. In addition, the proposed system provided an excellent analytical frequency (120 analysis h-1). The clinical feasibility of the proposed method was investigated by the determination of sAA levels in four saliva samples (two from healthy control persons (C1 and C2) and two from patients with chronic periodontitis (P1 and P2)). The accuracy provided by the BIA-AD system ranged from 93 to 98%. The sAA concentration levels achieved for each sample were compared to the values found by spectrophotometry and there was no statistically significant difference between them at a confidence level of 95%. Finally, the method reported herein emerges as a simple, low cost and promising tool for assisting periodontal diseases diagnosis.

Treatment of atrophic mandibular fracture related to implant placement in an oral bisphosphonate user: a case report / Tratamento de fratura em mandíbula atrófica com implantes em paciente usuário de bisfosfonato: umrelato de caso

Objectives: Atrophic mandibular fractures associated with placement of dental implants is an uncommon condition and to best of our knowledge this event in an oral bisphosphonate user was never described before. Case report: A 74-years-old woman presented a submandibular hematoma and mobility between two fragments on the right side of the body of the mandible after four implants placement. The patient reported the use of oralbisphosphonates for three years for treatment of osteoporosis. A titanium plate was placed at the base of the mandible to fix the fracture and the patient underwent a hyperbaric oxygen therapy for three months. Nine months after the surgery, the patient had no further complications and rehabilitation treatment was completed. Conclusions: The fracture fixation was effective in the treatment of atrophic mandibular fractures in an oral bisphosphonate user, with no occurrence of complications like osteonecrosis.In addition, the oral rehabilitation with prosthesisunder the remaining implants showed a satisfactory outcome.

Objetivo: Fraturas de mandíbula atrófica associadas à inserção de implantes é uma condição de ocorrência incomum e o objetivo desse relato de caso é descrever o tratamento de fratura de mandíbula atrófica associada à instalação de implantes em uma paciente usuária de bisfosfonato oral. Relato de caso: Paciente do sexo feminino com 74 anos apresentava presença de um hematoma submandibular e mobilidade entre dois fragmentos no corpo da mandíbula no lado direito após a instalação de 4 implantes. A paciente reportou uso de bisfosfonato por via oral a 3 anos para tratamento de osteoporose. A fratura foi reduzida e fixadacom uma placa de titânio na base da mandíbula e a paciente foi submetida a sessões de câmara hiperbárica por 3 meses. Após 9 meses do procedimento cirúrgico a paciente não apresentou complicações adicionais e o tratamento reabilitador foi finalizado. Conclusão: A fixação foi efetiva no tratamento da fratura em mandíbula atrófica em um paciente usuário de bisfosfonato oral e complicações com osteonecrose não foram detectadas. Adicionalmente, a reabilitação oral com próteses sobre implantes remanescente apresentaram um resultado satisfatório.

Topical application of the lectin Artin M accelerates wound healing in rat oral mucosa by enhancing TGF-ß and VEGF production.

The lectin Artin M has been shown to accelerate the wound-healing process. The aims of this study were to evaluate the effects of Artin M on wound healing in the palatal mucosa of rats and to investigate the effects of Artin M on transforming growth factor beta (TGF-ß) and vascular endothelial growth factor (VEGF) secretion by rat gingival fibroblasts. A surgical wound was created on the palatal mucosa of 72 rats divided into three groups according to treatment: C--Control (nontreated), A--Artin M gel, and V--Vehicle. Eight animals per group were sacrificed at 3, 5, and 7 days postsurgery for histology, immunohistochemistry and determination of the levels of cytokines, and growth factors. Gingival fibroblasts were incubated with 2.5 µg/mL of Artin M for 24, 48, and 72 hours. The expression of VEGF and TGF-ß was determined by enzyme-linked immunosorbent assay. Histologically, at day 7, the Artin M group showed earlier reepithelialization, milder inflammatory infiltration, and increased collagen fiber formation, resulting in faster maturation of granular tissue than in the other groups (p < 0.05). Artin M-induced cell proliferation in vivo and promoted a greater expression of TGF-ß and VEGF in both experiments (p < 0.05). Artin M was effective in healing oral mucosa wounds in rats and was associated with increased TGF-ß and VEGF release, cell proliferation, reepithelialization, and collagen deposition and arrangement of fibers.

Parotidite aguda em jovem adulto sistemicamente saudável. Relato de caso / Acute parotitis in a systemically healthy young adult. A case report

Objetivo: Este estudo relata um caso incomum de parotidite aguda em um paciente adulto jovem, saudável e com boa higiene oral. Relato de Caso: O paciente procurou o Departamento de Estomatologia, na Universidade Estadual de Ponta Grossa devido a um inchaço na região da glândula parótida direita. O paciente foi submetido a exames clínicos e radiológicos e foi prescrito tratamentos com drogas anti-inflamatórias e antibióticos. No quinto dia os sinais e sintomas haviam desaparecido. Conclusão: O desenvolvimento de parotidite aguda em adultos jovens saudáveis com uma boa higiene oral é possível, mas isso torna o diagnóstico mais difícil. Anti-inflamatórios, antibióticos e fisioterapia associada com calor pode contribuir para a recuperação dos sinais e sintomas após 5 dias de tratamento.

Aim: This study reports an unusual case of acute parotitis in a healthy young adult with good oral hygiene. Case Report: The patient sought the Department of Stomatology, at Ponta Grossa State University due to a swollen on the right parotid gland region. The patient underwent clinical and radiological treatments and was prescribed anti-inflammatory drugs and antibiotics. On the fifth day the signs and symptoms had disappeared. Conclusion: The development of acute parotitis in healthy young adults with good oral hygiene is possible, but this makes the diagnosis more difficult. Anti-inflammatory, antibiotic and heat physiotherapy can contribute to the recovery of the signs and symptoms after 5 days of treatment.

Efeito do stress no grau de inflamação gengival em adolescentes grávidas: estudo piloto / Effect of stress on the degree ofgingival inflammation in pregnant adolescents: pilot study

Objetivo: Avaliar a severidade de inflamação gengival perante duas condições, estresse e gravidez, em um grupo de adolescentes atendidas no serviço público de saúde na cidade de Maceió-AL. Material e métodos: 80 adolescentes grávidas com faixa etária ente 14 e 19 anos, atendidas em centros de referência ao atendimento pré-natal na cidade de Maceió-AL, participaram desse estudo piloto. O estresse social foi avaliado subjetivamente pelo teste de avaliação de estresse e a inflamação gengival foi avaliada pelo índice de inflamação gengival através de um exame clínico utilizando-se 6 dentes índices. A análise estatística foi executada relacionando o estresse social com dados demográficos e de hábitos de higiene oral e com relação a inflamação gengival pelo teste de Mann-Whitney e pelo teste de Fischer para variáveis dicotômicas. Resultados: Na avaliação da relação do estresse com a inflamação gengival e com o índice de placa com controle das variáveis independentes foi utilizada a regressão logística multivariada. Todos os testes foram aplicados com nível de confiança de 95%. O teste de Mann-Whitney demonstrou que as grávidas estressadas apresentavam maior frequência de escovação que as grávidas sem stress (p=0.008). Os outros dados demográficos e de hábitos de higiene oral se distribuíram igualmente entre os grupos. A análise por regressão logística multivariada demonstrou que nenhuma das variáveis independentes influenciou no índice gengival e no índice de placa em mulheres grávidas com ou sem stress. Conclusão: O estresse não causou aumento da severidade da inflamação gengival em adolescentes grávidas.

Objective: Evaluate the severity of gingival inflammation against two conditions, stress and pregnancy, in a group of adolescents treated at a public health service in Maceió-AL. Material and methods: 80 pregnant adolescents aged between 14 to 19 years being treated at referral centers for prenatal care in the city of Maceió-AL, participated in this pilot study. The social stress was subjectively evaluated by assessment of stress test (Lipp test) and gingival inflammation was assessed by the index of gingival inflammation (Loe & Silness) through a clinical examination using six index teeth. Statistical analysis was performed relating the social stress with demographic data and habits regarding oral hygiene and gingival inflammation using the Mann-Whitney and Fischer's test for dichotomous variables. Results: When considering the influence of the stress in gingival inflammation and plaque index with controlled of theindependent variables the multivariate logistic regression was used. All tests were applied with a confidence level of 95%. The Mann-Whitney test demonstrated that pregnant women under stress showed higher frequency of brushing than pregnant women without stress (p = 0.008). The other demographic and oral hygiene habits were equally distributed between the groups. A multivariate logistic regression analysis showed that none of the independent variables influenced the gingival index and plaque index in pregnant women with or without stress. Conclusion:The stress did not increase the severity of gingival inflammation in pregnant adolescents.

Efeito da aplicação de adesivos de 2 passos e autocondicionantes fotoativados por luz halógena ou LED na microinfiltração marginal: estudo in vitro. / Effect of 2-step/self-each bond system and halogen light or LED on the microleakage: in vitro study.

O objetivo desse estudo foi analisar a efetividade de sistemas adesivos convencionais e autocondicionantes, e de diferentes métodos de fotoativação para redução da microinfiltração marginal. Foram utilizados 48 dentes incisivos bovinos nesse estudo. Os dentes foram divididos em quatro grupos de 12 amostras de acordo com o tipo de adesivo ou método de fotoativação utilizado: Grupo 1- Sistema adesivo de passos fotoativadas por luz halógena com intensidade de 700 mW/cm2 Grupo 2 - Sistema adesivo de dois passos fotoativados pelo LED com intensidade de 470 mW/cm2; Grupo 3 - sistema adesivo autocondicionante fotoativados por luz halógena; Grupo 4 - Sistema adesivo autocondicionante fotoativados por LED. Uma resina composta nanoparticulada foi utilizada para restaurar todas as cavidades. Em seguida, os grupos foram submetidos ao tratamento de ciclagem térmica e foram colocados por 24 horas em solução de azul de metileno. A microinfiltração foi avaliada de acordo com o grau de penetração do corante na interface dente-restauração. Para análise estatística, foi utilizado o teste não paramétrico de Kruskal Wallis complementado pelo teste de Mann-Whitney, no nível de 5% de significância. O grupo 1 apresentou estatisticamente menores índices de microinfiltração marginal que o grupo 2 na parece oclusal (p<0.05) e os grupos 1 e 4 apresentaram menores graus de microinfiltração marginal que o grupo 2 na parede cervical(p<0.05). A aplicação do sistema adesivo de dois passos associado a polimerização com luz halógena promoveram os menores graus de microinfiltração marginal.

The aim of this research was to analyze the effectiveness of conventional and self-etching bonding systems and different curing methods for reduction of marginal microleakage. 48 bovine incisor teeth were used in this study. The teeth were divided into four groups of 12 samples according to the type of adhesive system or curing method used: Group 1- Two-step bonding system photoactivated by halogen light with intensity of 700 mW/cm2; Group 2- Two-step bonding system photoactivated by LED with 470 mW/ cm2 of intensity; Group 3 - Self etch bonding system photoactivated by halogen light; Group 4- Self etch bonding system photoactivated by LED. A nanofilled composite resin was used to restore all the cavities. Then the groups were subjected to thermal cycling treatment and were placed for 24 hours in a methylene blue solution. The microleakage was assessed according to the degree ofpenetration of the colorant in tooth-restoration interface. For statistical analysis, the non-parametric Kruskal Wallis test complemented by Mann-Whitney were used with 5 % level of significance. The Group 1 showed lower marginal microleakage than Group 2 on occlusal wall (p<0.05). Adittionally, appears and the groups 1 and 4 had smaller degrees of marginal microleakage that the cervical wall 2 Group (p<0.05). The application of two-step bonding system associated with the polymerization by halogen light promoted the lowest degrees of marginal microleakage.

Interleukin-4 and interleukin-13 inhibit the expression of leukemia inhibitory factor and interleukin-11 in fibroblasts.

Cytokines produced by inflammatory or resident mesenchymal cells play important modulatory roles in the pathogenesis of inflammation induced bone loss. In the present study, the effects of IL-4 and IL-13 on the expression of three osteotropic cytokines in the IL-6 family expressed in human gingival fibroblasts were studied. IL-4Rα and IL-13Rα1 mRNA were constitutively expressed in human gingival fibroblasts. The inflammatory cytokines IL-1ß and TNF-α increased expression of IL-6, LIF, and IL-11 mRNA and protein in the gingival fibroblasts. Addition of IL-4 or IL-13 had no effect on IL-6 expression, but significantly inhibited LIF and IL-11 mRNA and protein stimulated by IL-1ß and TNF-α. No involvement of NF-κB or STAT1 was observed in the inhibition. STAT6 was phosphorylated at Y641 by treatment with IL-4 and knockdown of STAT6 with siRNA decreased the inhibition of IL-11 and LIF expression by IL-4 in IL-1ß and TNF-α stimulated cells. This study suggests that activation of STAT6 by IL-4 and IL-13, through type 2 IL-4 receptors, inhibits production of IL-11 and LIF stimulated by IL-1ß and TNF-α in human gingival fibroblasts. A negative modulatory role of IL-4 and IL-13 in osteotropic cytokine production could be a mechanism playing an important inhibitory role in inflammation induced periodontitis.