The binding of cadmium to crab cadmium metallothionein. A polarographic investigation.

Differential pulse polarography was used to monitor the titration of crab (Cancer pagurus) cadmium metallothionein with additional cadmium. Scatchard analysis was used to calculate an apparent second-order dissociation constant. A mean value of 3.60 x 10(-7) M was calculated at 20 degrees C in 0.016 M-NaClO4, pH 8.0.