Presence and expression of apelin and apelin receptor in bitch placenta.

Apelin is a potent inotropic agent causing endothelium-mediated vasodilation and is involved in vessel formation by interacting with a specific receptor. Its cardiovascular profile suggests a role in the regulation of gestational hemodynamic changes. The expression of apelin and its receptor has been reported in some portions of the reproductive tract of different mammalian species. As far as we know, there are no reports describing the expression of apelin and apelin receptor in bitch's placenta. Therefore, the aim of this study was to investigate, for the first time, the presence and distribution of apelin and apelin receptor in bitch placenta by molecular biology and immunohistochemical techniques. Sixteen adult female half-breed bitches were used. The animals were divided into two groups based on the stage of pregnancy: group 1 (mid-gestation n = 8) and group 2 (end gestation n = 8). These bitches were subjected to ovariohysterectomy (group1) or non-conservative caesarean section (group 2). The immunohistochemical technique revealed the presence of positive immune reaction for apelin and apelin receptor in all the samples examined at 30 days and at the end of pregnancy. In particular, apelin and apelin receptor staining was evident in the cytoplasms of cytotrophoblasts and in epithelial cells of the maternal portion. Even if not included into the structure of the placenta, the uterine glands also exhibited a positive immune reaction for apelin and apelin receptor. The RT-PCR analysis showed the presence of transcripts for apelin and apelin receptor in all the placenta samples examined. On the basis of our results it was also possible to hypothesize a potential role of apelin in the control of local placenta blood flow during pregnancy development in bitches.

Fatal bronchopneumonia in a Metastrongylus elongatus and Porcine circovirus type 2 co-infected pig.

Porcine circovirus type 2 (PCV2) infection is distributed worldwide and PCV2-associated disease (PCVAD) is considered among the most economically relevant ones to the global swine industry. PCV2 is known to play a causal role in the porcine respiratory disease complex, usually in close association with a large plethora of other biologic agents. We describe herein a case of fatal parasitic bronchopneumonia by Metastrongylus elongatus in a PCV2-infected pig. Metastrongylosis may still represent a major concern for outdoor herds. Our recent experience suggests that a concurrent PCVAD condition may trigger metastrongylosis, which may subsequently result, at its turn, in severe, sometimes fatal, pulmonary disease.

A role of TNF-alpha gene variant on juvenile ischemic stroke: a case-control study.

The role of genetic factors in the individual predisposition to develop ischemic stroke has been assessed by previous studies performed both in animal models and in humans. The main goal of the current investigation was to determine the possible contribution of genes encoding procoagulant and inflammatory factors on the occurrence of ischemic stroke in a cohort of young cases and corresponding controls. One hundred and fifteen cases of ischemic stroke were recruited for this study. A detailed clinical assessment, a definite etiologic diagnosis, as well as the presence/absence of known risk factors for ischemic stroke were obtained for each patient. As a control group 180 healthy, unrelated subjects were included. The whole population was screened for polymorphisms belonging to genes encoding FII, FV, alpha-fibrinogen, beta-fibrinogen, GP IIb/IIIa, tumor necrosis factor (TNF)-alpha, interleukin 1-beta. Hypertension was the most important risk factor for ischemic stroke in our cohort [OR = 6.9, confidence interval (CI) 2.9-16.7, P < 0.0001]. Among all genes tested, the TNF-alpha gene variant exerted a significant, independent effect on individual predisposition to ischemic stroke occurrence (OR = 1.8, CI = 1.01-3.3, P < 0.05). Our findings, obtained in a cohort of young Italian patients, may support the existence of a direct contributory role of TNF-alpha, a proinflammatory cytokine protein, in the susceptibility to brain damage.

Perforación apendicular en la infancia. ¿Es posible su prevención? / Appendicceal perforation en childhood. Is its prevention possible?

Fueron estudiados en el Servicio de Pediatría del Hospital Interzonal General de Agudos Dr.J.Penna de Bahía Blanca, 166 niños operados deapendicitis aguda en febrero de 1990 y febrero de 1995, con los fines de establecer la tasa de perforación apendicular, las posibles causas y delinear la forma de disminuirla. Se operaron 60 niñas (36,1 por ciento), y 106 varones (63,9 por ciento) con una edad promedio de 9,24 años (r=1-14 años).Los síntomas prevalentes fueron dolor abdominal (100 por ciento) los vómitos (69,27 por ciento) y la fiebre (52,4 por ciento). Se registraron 41 consultas pediátricas previas donde se efectuó un diagnóstico erróneo, o bien, se omitió advertir un cuadro de origen apendicular. En ninguno de estos casos se realizó interconsulta quirúrgica y en 32 de los mismos se prescribió por lo menos una medicación.Los hallazgos intraoperatorios incluyeron 13 apendicitis congestivas,69 flegmonosas, 28 gangrenosas,32 peritonitis localizadas y 24 peritonitis difusas.El tiempo transcurrió entre el inicio de los síntomas hasta la intervención quirúrgica fue de 1,96 días, con un rango de 10 hs a 10 días. El tiempo de evolución calculado para quienes presentaban un diagnóstico erróneo fue de 2,9 1,68 días contra 1,64 1,17 días, de quienes tuvieron un primer diagnóstico correcto.La tasa global de perforaciones fue del33,73 por ciento (56/166) mientras que la discriminada por edades correspondió al 46,15 por ciento para el grupo de 0-8 años (30/65) y al25,7 por ciento para el grupo de 9-14 años (26/101) . La tasa de peritonitis para quienes registraban antecedentes del error diagnóstico fue del 70,73 por ciento (29/41), versus el 21,6 por ciento de quienes fueron diagnosticados en la primera consulta (27/125). Se demostró una asociación estadísticamente significativa entre la perforación apendicular y la demora en el diagnóstico, independientemente de las edades de los pacientes (p< 0,005) .El tiempo de internación varió entre 1 y 34 días, con un promedio de 5,74 días, no registrándose mortalidad en esta serie. Se presentaron complicaciones en 26 niños, de los cuales 7 requirieron un reintervención quirúrgic

Perforación apendicular en la infancia. ¿Es posible su prevención? / Appendicceal perforation en childhood. Is its prevention possible?

Fueron estudiados en el Servicio de Pediatría del Hospital Interzonal General de Agudos Dr.J.Penna de Bahía Blanca, 166 niños operados deapendicitis aguda en febrero de 1990 y febrero de 1995, con los fines de establecer la tasa de perforación apendicular, las posibles causas y delinear la forma de disminuirla. Se operaron 60 niñas (36,1 por ciento), y 106 varones (63,9 por ciento) con una edad promedio de 9,24 años (r=1-14 años).Los síntomas prevalentes fueron dolor abdominal (100 por ciento) los vómitos (69,27 por ciento) y la fiebre (52,4 por ciento). Se registraron 41 consultas pediátricas previas donde se efectuó un diagnóstico erróneo, o bien, se omitió advertir un cuadro de origen apendicular. En ninguno de estos casos se realizó interconsulta quirúrgica y en 32 de los mismos se prescribió por lo menos una medicación.Los hallazgos intraoperatorios incluyeron 13 apendicitis congestivas,69 flegmonosas, 28 gangrenosas,32 peritonitis localizadas y 24 peritonitis difusas.El tiempo transcurrió entre el inicio de los síntomas hasta la intervención quirúrgica fue de 1,96 días, con un rango de 10 hs a 10 días. El tiempo de evolución calculado para quienes presentaban un diagnóstico erróneo fue de 2,9 1,68 días contra 1,64 1,17 días, de quienes tuvieron un primer diagnóstico correcto.La tasa global de perforaciones fue del33,73 por ciento (56/166) mientras que la discriminada por edades correspondió al 46,15 por ciento para el grupo de 0-8 años (30/65) y al25,7 por ciento para el grupo de 9-14 años (26/101) . La tasa de peritonitis para quienes registraban antecedentes del error diagnóstico fue del 70,73 por ciento (29/41), versus el 21,6 por ciento de quienes fueron diagnosticados en la primera consulta (27/125). Se demostró una asociación estadísticamente significativa entre la perforación apendicular y la demora en el diagnóstico, independientemente de las edades de los pacientes (p< 0,005) .El tiempo de internación varió entre 1 y 34 días, con un promedio de 5,74 días, no registrándose mortalidad en esta serie. Se presentaron complicaciones en 26 niños, de los cuales 7 requirieron un reintervención quirúrgic

Ketorolac versus diclofenac sodium in cancer pain.

In a randomized single-blind study carried out simultaneously in five Departments for Pain Therapy and Palliative Care, the analgesic efficacy and side effects of oral ketorolac (ketorolac tromethamine, Tora-Dol, CAS 74103-07-4) and diclofenac sodium were compared in a population of 100 advanced cancer patients suffering from somatic and/or visceral pain. The treatment was carried out in agreement with the first step of the WHO pharmacological strategy in cancer pain. The administered dosage was 10 mg every 6 h for ketorolac and 50 mg every 8 h for diclofenac sodium. The study showed the efficacy of both drugs in cancer pain. A greater number of keterolac patients could pass to the second WHO step later than diclofenac patients. As to the tolerability, both drugs turned out to be similar, except for "sleepiness", which was four times more frequent (p < 0.05) in the diclofenac group.

[Trazodone versus flunitrazepam in premedication in day-care surgery]. / Trazodone versus flunitrazepam nella premedicazione in regime di ricovero giornaliero.

A prospective single-blind study was conducted to compare flunitrazepam vs trazodone in the premedication of patients undergoing day-case surgery for termination of pregnancy, with particular regard to the degree of preoperative sedation, intraoperative analgesia and postoperative recovery. 86 patients were randomly allocated to receive orally 45 minutes before the surgical procedure either flunitrazepam 2 mg (group F) or trazodone 50 mg (group T). In both groups anaesthesia was achieved by i.v. fentanyl 2.5 micrograms/kg and ketamina 250 micrograms/kg. Patients in group F showed a deeper degree of preoperative sedation. There were no significant differences in intraoperative analgesia and in the immediate arousal time. In the postoperative period, the incidences of emetic symptoms and dizziness were similar in both groups; the incidence of drowsiness was significantly higher in group F at 120 minutes but not at 180 minutes of observation. Psychomotor performance was assessed preoperatively two days before the surgical procedure and 60, 120 and 180 minutes after surgery, using the Toulouse-Pieron test and the reaction time to a luminous stimulus with the aid of a computerized analogic tachystoscope (Neurometer). Trazodone allowed a more rapid recovery of psychomotor performance and it can represent a valid alternative to the use of benzodiazepines in the premedication of day-case surgical patients.

[Oxycodone versus paracetamol in oral premedication in cholecystectomy]. / Ossicodone versus paracetamolo in premedicazione per os negli interventi di colecistectomia.

This double-blind study was carried out at random on 2 groups of 10 women, comparable by age and weight, all having to undergo a cholecystectomy as a result of gall-bladder stones. Group A received 10 mg os of oxycodone, while group B received 1000 mg os of paracetamol. The level of sedation before the operation, post-operative pain, canalization and the side effect were recorded. The results showed that the group treated with oxycodone presented a greater degree of pre-operative sedation. As far as the post-operative pain is concerned, no significant differences were seen between the two groups based on both what was expressed by the patients and what was reported by a visual analog. Furthermore, no significant differences were noted as far as the side effects and canalization are concerned.

[Clebopride in premedication in ambulatory interventions in general anesthesia]. / La clebopride nella premedicazione degli interventi ambulatoriali in anestesia generale.

The authors examine the antiemetic effects of 1 mg clebopride administered iv after surgery, vs a placebo, by making a double blind randomized study on two groups of 40 women comparable by age and weight. The 2 groups of outpatients, admitted for short gynecological surgery, underwent diagnostic uterine curettage. They were anaesthetized with a cocktail of 2.5 mcg/kg fentanyl and 0.25 mg/kg ketamine, on spontaneous respiration. Nausea, vomiting and the other side effects were evaluated 3-6 hours after surgery. Statistically, clebopride proved more effective than placebo against nausea and vomiting (P ranging between 0.05-0.01), with no relevant side effects.

Sodium naproxen versus sodium diclofenac in cancer pain control.

In a single-blind random study, simultaneously carried out by five Pain Therapy and Palliative Care Centres, the analgesic power and side-effects of sodium naproxen (CAS 26159-34-2) and sodium diclofenac (CAS 15307-86-5) by mouth were compared in a group of 100 advanced cancer patients. The patients complained of somatic and/or visceral pain and were treated with non-steroid anti-inflammatories as required. The dose administered amounted to 550 mg every 12 h for sodium naproxen and to 100 mg every 12 h for sodium diclofenac. The study stressed the similar analgesic effect of the two drugs--pain intensity and duration decreased by half in the first week of treatment--and a comparatively low morbidity rate.