Phosphorus-Induced Adaptation Mechanisms of Rye Grown on Post-Flotation Copper Tailings.

Although a considerable effort has been made over the last decades to develop cost-effective phytotechnologies as an alternative to conventional techniques for the management of contaminated lands, successful revegetation of the tailings still represents a major challenge. Here, we evaluate the potential of rye (Secale cereale L.) for growth and survival on the tailings after copper (Cu) ore processing. Four rye varieties were cultivated in a pot experiment on the post-flotation sediment with increasing phosphorus (P) doses (22, 44, 66, 88, and 110 mg·kg-1). The resistance of the studied rye genotypes to stress was assessed by observing the growth and development of plants, determining the dry mass accumulation, the Cu and P uptake and content, and a number of physiological parameters related mainly to P mobilisation. Exposure of tested rye varieties to high Cu concentrations in the tailings did not result in any significant plant mortality, with the intracellular Cu concentrations being below the critical toxic level. In contrast, the low availability of P due to alkaline properties of the tailings and the mechanisms involved in the mobilisation of sparingly soluble forms of this element (i.e., H+-ATPase-driven proton efflux in roots and organic acid exudation), were identified as main factor determining the level of tolerance. The efficiency of the photosynthetic activity was a key determinant for the P-mobilising capacity of rye. We further showed that rye varieties with more primitive genetic background might be potentially more suitable for growth on the post-flotation copper tailings. The results provide important and novel knowledge that will certainly support future works in developing strategies for successful revitalisation of degraded lands.

Correction to: Heavy metal and nutrient uptake in plants colonizing post-flotation copper tailings.

The correct presentation of Table 4 is shown in this paper.

Heavy metal and nutrient uptake in plants colonizing post-flotation copper tailings.

Copper ore mining and processing release hazardous post-flotation wastes that are difficult for remediation. The studied tailings were extremely rich in Cu (1800 mg kg-1) and contaminated with Co and Mn, and contained very little available forms of P, Fe, and Zn. The plants growing in tailings were distinctly enriched in Cu, Cd, Co, Ni, and Pb, and the concentration of copper achived the critical toxicity level in shoots of Cerastium arvense and Polygonum aviculare. The redundancy analysis demonstrated significant relationship between the concentration of available forms of studied elements in substrate and the chemical composition of plant shoots. Results of the principal component analysis enabled to distinguish groups of plants which significantly differed in the pattern of element accumulation. The grass species Agrostis stolonifera and Calamagrostis epigejos growing in the tailings accumulated significantly lower amounts of Cu, but they also had the lowest levels of P, Fe, and Zn in comparison to dicotyledonous. A. stolonifera occurred to be the most suitable species for phytostabilization of the tailings with regard to its low shoot Cu content and more efficient acquisition of limiting nutrients in relation to C. epigejos. The amendments improving texture, phosphorus fertilization, and the introduction of native leguminous species were recommended for application in the phytoremediation process of the tailings.