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1.
Sensors (Basel) ; 20(2)2020 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-31952367

RESUMO

The aim of the study was to examine the biochemical and structural changes occurring in the periodontal ligament (PDL) during orthodontic-force application using micro-Raman spectroscopy ( µ -RS). Adolescent and young patients who needed orthodontic treatment with first premolar extractions were recruited. Before extractions, orthodontic forces were applied using a closed-coil spring that was positioned between the molar and premolar. Patients were randomly divided into three groups, whose extractions were performed after 2, 7, and 14 days of force application. From the extracted premolars, PDL samples were obtained, and a fixation procedure with paraformaldehyde was adopted. Raman spectra were acquired for each PDL sample in the range of 1000-3200 cm - 1 and the more relevant vibrational modes of proteins (Amide I and Amide III bands) and CH 2 and CH 3 modes were shown. Analysis indicated that the protein structure in the PDL samples after different time points of orthodontic-force application was modified. In addition, changes were observed in the CH 2 and CH 3 high wavenumber region due to local hypoxia and mechanical force transduction. The reported results indicated that µ -RS provides a valuable tool for investigating molecular interchain interactions and conformational modifications in periodontal fibers after orthodontic tooth movement, providing quantitative insight of time occurring for PDL molecular readjustment.


Assuntos
Ligamento Periodontal , Análise Espectral Raman/métodos , Técnicas de Movimentação Dentária , Adolescente , Adulto , Feminino , Humanos , Masculino , Ligamento Periodontal/química , Ligamento Periodontal/fisiologia , Proteínas/análise , Proteínas/química , Adulto Jovem
2.
J Rheumatol ; 29(1): 94-101, 2002 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-11824978

RESUMO

OBJECTIVE: To elucidate events that initiate the involvement and stimulation of fibroblasts in systemic sclerosis (SSc). METHODS: We examined 15 patients with SSc diffuse form, 15 with CREST syndrome, and 5 healthy subjects. Cultured fibroblasts obtained from skin biopsies in SSc involved and non-involved areas and norrmal skin fibroblasts were cultured with different doses of granulocyte macrophage colony stimulating factor (GM-CSF) to study the effects of this factor on the expression of GM-CSF receptor (GM-CSFR) on fibroblast proliferation and cellular adhesion structures. RESULTS: Cultured fibroblasts obtained from biopsies of normal and SSc skin areas express GM-CSFR and such expression is increased in SSc fibroblasts. GM-CSF stimulation in vitro did not increase SSc fibroblast growth, in spite of a strongly increased expression of the GM-CSFR. The adhesion structures are always more abundant in SSc fibroblasts as compared to healthy cells and GM-CSF seems able to increase cell adhesion plaques. CONCLUSION: We suggest that shift of fibroblasts toward a more adhesive differentiated pattern, due to or accompanied by an increased expression of GM-CSFR, may be an important event in the pathogenesis of SSc.


Assuntos
Síndrome CREST/metabolismo , Divisão Celular/efeitos dos fármacos , Derme/metabolismo , Fibroblastos/metabolismo , Fator Estimulador de Colônias de Granulócitos e Macrófagos/metabolismo , Receptores de Fator Estimulador das Colônias de Granulócitos e Macrófagos/metabolismo , Regulação para Cima/imunologia , Adulto , Idoso , Síndrome CREST/imunologia , Síndrome CREST/fisiopatologia , Adesão Celular/efeitos dos fármacos , Adesão Celular/imunologia , Divisão Celular/imunologia , Membrana Celular/imunologia , Membrana Celular/metabolismo , Células Cultivadas , Derme/imunologia , Derme/fisiopatologia , Feminino , Fibroblastos/imunologia , Expressão Gênica/imunologia , Fator Estimulador de Colônias de Granulócitos e Macrófagos/imunologia , Fator Estimulador de Colônias de Granulócitos e Macrófagos/farmacologia , Humanos , Imuno-Histoquímica , Masculino , Pessoa de Meia-Idade , Receptores de Fator Estimulador das Colônias de Granulócitos e Macrófagos/imunologia , Frações Subcelulares/imunologia , Frações Subcelulares/metabolismo
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