RESUMO
Neste estudo é apresentada uma revisão sobre nó-de-cachorro (Heteropterys tomentosa A. Juss.), espécie de uso medicinal em Mato Grosso. São abordadas as características da espécie com a descrição da planta, hábitat, distribuição geográfica, ecologia, fenologia e biologia reprodutiva, propriedades químicas e farmacológicas. Os aspectos como a etnobotânica (uso e partes usadas), formas de exploração atual e informações sobre cultivo são discutidos.
This study presents a review about "Nó-de-cachorro" (Heteropterys tomentosa A. Juss.), a medicinal species from Mato Grosso State, Brazil. We outlined the characteristics of the species including the plant description, habitat, geographic distribution, ecology, phenology and reproductive biology, chemical and pharmacological properties. Aspects such as ethnobotany (use and used parts), current ways of exploiting and information on cultivation are discussed.
Assuntos
Brasil , Malpighiaceae , Plantas Medicinais/crescimento & desenvolvimento , Produção Agrícola , Etnobotânica , Etnofarmacologia , PradariaRESUMO
In biological research, quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR) assays are commonly employed to study mRNA abundance in cells and tissues. This type of assay usually relies on assessing transcript abundance relative to constitutively expressed endogenous reference genes. Therefore, it is important that the reference genes themselves are stably expressed in the cells or tissues analyzed, independent of factors such as age, sex, disease or experimental manipulations. Since no gene is expressed at the same level in all cells at all times, suitable reference genes must be identified for the specific cellular system or tissue being investigated. Here, we sought to identify stably expressed endogenous reference genes during embryonic gonad development in the mouse. We measured the transcript abundance of 10 frequently employed normalizing genes, of which 4 were stably expressed in fetal gonads from 11.5 to 14.5 dpc irrespective of sex. Based on our analysis, we suggest that Rn18s, Rps29, Tbp and Sdha are suitable reference genes for qRT-PCR expression studies during early gonad differentiation in the mouse.
