RESUMO
The level of gene polymorphism of 10 microsatellite loci in 27 American Pit Bull Terrier dogs which have a high value of the percentage of blood and inbreeding coefficient achieved 43.8% was studied. The excess of heterozigosity over expected for this level of inbreeding coefficient was established. Suggestion that the high level of heterozigosity is the result of the selection against homozygotes was made.
Assuntos
Cães/genética , Endogamia , Repetições de Microssatélites/genética , Polimorfismo Genético , Animais , Animais Endogâmicos , Marcadores Genéticos , Heterozigoto , LinhagemRESUMO
A diagnostic kit for detection of avian influenza virus by real-time polymerase chain reaction was developed. This kit allows to identify the influenza viruses type A and highly pathogenic strain of avian influenza virus H5N1. The diagnostic kit is universal and adopted for ABI PRISM SDS (Applied Biosystems), RotorGene (Corbett Research) and iQCycler (BioRad) PCR machines.
Assuntos
Virus da Influenza A Subtipo H5N1 , Influenza Aviária/virologia , Reação em Cadeia da Polimerase/métodos , Sequência de Aminoácidos , Animais , Aves , Virus da Influenza A Subtipo H5N1/genética , Virus da Influenza A Subtipo H5N1/isolamento & purificação , Virus da Influenza A Subtipo H5N1/patogenicidade , Dados de Sequência Molecular , RNA Viral/genética , Reprodutibilidade dos Testes , Sensibilidade e Especificidade , VirulênciaRESUMO
Hybridization analysis of the double-strand RNA-elements isolated from the sugar beet of the Kyiv, Vinnytsya and Poltava Regions was carried out. A positive signal has been obtained with the dsRNA fragment, 1.9 kbp long, which codes the viral RNA- dependent RNA-polymerase having high level of homology with the analogous enzyme of mycoviruses from Partitiviridae family. Molecular identity of these dsRNA-elements in the sugar beet samples from the Vinnytsya and Poltava regions has been shown The RT-PCR test system has been developed for detecting the nondescribed mycovirus which is proposed to be called Helicobasidium purpureum partitivirus.
Assuntos
Beta vulgaris/virologia , Vírus de Plantas/genética , Vírus de RNA/genética , Vírus de RNA/isolamento & purificação , RNA de Cadeia Dupla/genética , Sequência de Aminoácidos , Sequência de Bases , Sequência Conservada , Dados de Sequência Molecular , Hibridização de Ácido Nucleico , Vírus de Plantas/química , Vírus de RNA/classificação , RNA de Cadeia Dupla/química , RNA Viral/genética , RNA Polimerase Dependente de RNA/química , RNA Polimerase Dependente de RNA/genética , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Homologia de Sequência de Aminoácidos , Homologia de Sequência do Ácido NucleicoRESUMO
We used yeast three-hybrid system, for studying interaction of alfalfa mosaic virus coat protein AMVCP (AMVCP) with RNA4, which codes this protein. We have shown that AMVCP with high affinity is bound to plus-chain of RNA4 in vivo. The mutational analysis has shown, that the N-terminal part of AMVCP (aa 1 to 85) contains RNA-binding domain. C-terminal part of this protein (aa 86 to 221) does not participate in direct interaction with RNA4. However activity of the reporter-gene LacZ, which codes beta-galactosidase, in case of interaction only N-terminal part of AMVCP is five times lower, in comparison with full-length hybrid protein, that confirms that the tertiary structure of full-length AMVCP is more favourable for interaction with RNA4.
