RESUMO
18S-25S rDNA of intact plants and tissue cultures of G. acaulis, G. punctata and G. lutea have been investigated by using blot-hybridization. The decrease of rDNA amount was found in the callus cultures as compared with the plants. In contrast to other species, G. lutea showed intragenome heterogeneity of rRNA genes as well as qualitative rDNA changes in tissue culture, in particular appearance of altered repeats. The relationship between the peculiarities of rRNA gene structure and their rearrangements in in vitro culture was suggested.
Assuntos
DNA de Plantas/genética , DNA Ribossômico/genética , Gentiana/genética , Polimorfismo de Fragmento de Restrição , RNA Ribossômico 18S/genética , RNA Ribossômico/genética , Eletroforese em Gel de Ágar , Hibridização In Situ , Técnicas de Cultura de TecidosRESUMO
An analysis of 18S-25S and 5S rRNA genes in intact plants and cultured tissues of some Rauwolfia species was performed to compare these sequences variability occurred as a result of the species evolution in nature and that induced by tissue culture. The restriction fragment length polymorphism of 18S-25S and 5S rDNA was found both in intact plants of various Rauwolfia species and in long-term Rauwolfia serpentina tissue cultures. In addition, changes in the amount of 18S-25S rRNA genes were observed in long-term R. serpentina tissue cultures. The results demonstrate that rDNA variability observed in intact plants as well as in long-term cultures is attributed to differences in the same regions of ribosomal RNA genes.
Assuntos
Rearranjo Gênico/genética , Genes de Plantas , Genes de RNAr , Polimorfismo Genético , Rauwolfia/genética , DNA Ribossômico/genética , Desoxirribonuclease BamHI/metabolismo , Polimorfismo de Fragmento de Restrição , RNA Ribossômico/genética , RNA Ribossômico 18S/genética , RNA Ribossômico 5S/genética , Especificidade da Espécie , Técnicas de Cultura de TecidosRESUMO
18S-25S rDNA sequence in genomes of G. lutea plants from different natural populations and from tissue culture has been studied with blot-hybridization method. It was shown that ribosomal repeats are represented by the variants which differ for their size and for the presence of additional HindIII restriction site. Genome of individual plant usually possesses several variants of DNA repeats. Interpopulation variability according to their quantitative ratio and to the presence of some of them has been shown. Modifications of the range of rDNA repeats not exceeding intraspecific variability were observed in callus tissues in comparison with the plants of initial population. Non-randomness of genome modifications in the course of cell adaptation to in vitro conditions makes it possible to some extent to forecast these modifications in tissue culture.
Assuntos
DNA de Plantas/genética , DNA Ribossômico/genética , Genes de Plantas/genética , Variação Genética/genética , Gentiana/genética , RNA de Plantas/genética , RNA Ribossômico/genética , RNA Ribossômico 18S/genética , Sequências Repetitivas de Ácido Nucleico/genéticaRESUMO
The comparative study of the genomes of intact plants-representatives of some species of the genus Gentiana L. as well as cultured cells of G. lutea and G. punctata was performed using restriction analysis. Species specificity of restriction fragment patterns for studied representatives of this genus was revealed. The differences between electrophoretic patterns of digested DNA purified from rhizome and leaves of G. lutea and G. punctata were found. The changes in genomes of G. lutea and G. punctata cells cultured in vitro compared with the genomes of intact plants were detected. The data obtained evidence that some of them may be of nonrandom character.
