RESUMO
Of 790 samples of oyster shellstock freshly harvested during a 12-month survey, 111 (most of which were harvested from June through August) contained Vibrio cholerae non-O1 (611 strains), and seven contained O1 Inaba (11 strains) organisms. None of the V. cholerae strains isolated were enterotoxigenic by immunological and biological tests.
Assuntos
Microbiologia de Alimentos , Ostreidae/microbiologia , Vibrio cholerae/isolamento & purificação , Animais , Toxina da Cólera/biossíntese , Vibrio cholerae/classificação , Vibrio cholerae/metabolismoRESUMO
A cylindrical glass culture vessel equipped with a vented nylon closure was developed and evaluated for sterility testing. The sterility testing cylinder (STC), made in 2 sizes, accommodates 250 or 400 mL culture broth. A tight-fitting, skirted nylon cap protects the upper part of the cylinder, and a membrane filter in a recessed opening allows venting during autoclaving and ensures sterility during removal and replacement of the cap. The configuration of the vessel in terms of the opening size and ratio of the horizontal to vertical cross section provides depths desirable for testing many shapes of medical devices, including elongated and narrow ones, without excessive waste of medium. They are easy to charge with medium, and to autoclave, store, inoculate, and observe for growth.
Assuntos
Técnicas Bacteriológicas/instrumentação , Bandagens , Equipamentos e Provisões , Esterilização , Bactérias/crescimento & desenvolvimento , Meios de CulturaRESUMO
A strain of Vibrio parahaemolyticus, serotype O4/K4, that was Kanagawa positive and reactive in the rabbit ileal loop test, was isolated in low numbers from raw oysters implicated in a case of acute gastroenteritis. To our knowledge, this is the first report of the isolation of a Kanagawa-positive strain from suspect food in the United States.
