Long-term changes in microbial water quality indicators in a hydro-power plant reservoir: The role of natural factors and socio-economic changes.

Long-term changes, from 1984 to 2010, in the indicators of microbial pollution (total viable count, coliforms, Escherichia coli, enterococci, and Clostridium perfringens) are analysed in the Riga Hydropower Plant Reservoir, an essential source of drinking water for Riga, the capital of Latvia. Counts in microbial indicators fluctuated seasonally and were related to physicochemical parameters (nitrogen compounds, turbidity, temperature, and pH). The changes in microbial pollution were brought about by two major socio-economic developments. Firstly, Latvia's independence from the USSR in 1991 which facilitated a distinct reduction in most microorganism counts due to a sharp decline in industrial and agricultural production. This resulted in a significant drop in point and nonpoint pollution in the river basin. A further development was Latvia joining the European Union in 2004. The corresponding focus on water management, including wastewater treatment, was a major priority of environmental investment and lead to improvements in microbial water quality.

Multi-marker study of the responses of the Unio tumidus from the areas of small and micro hydropower plants at the Dniester River Basin, Ukraine.

The impact of the hydropower plants (HPPs) on the aquatic life is expected, but the biochemical markers of ecotoxicity have not been investigated in relation to HPP proximity. The aim of this study was to compare the responses of mollusk Unio tumidus from the small HPP (reservoir (Ku) and downstream of the dam (Kd)) and micro HPP (upstream (Zu) and downstream of the dam (Zd)). In total, 11 indexes (n) from digestive gland, hemocytes (lysosomal integrity), and gonads (alkali-labile phosphates, ALP) were analyzed. The mollusks from the reservoir demonstrate the typical signs of toxic impact: cholinesterase and glutathione depletion, the highest glutathione S-transferase activity, and ratio of extralysosomal cathepsin D compare to all other groups. The specimens from the micro HPP have the highest levels of glutathione (Zd) and lipid peroxidation (Zu) and lesser Cu/Zn-SOD activity (Zu) than other groups. These indications of stressful conditions may derive from the regular oscillations in the water flow regimes at the micro HPP. For both HPPs, the responses of upstream and downstream groups are distinct. The calculated IBR/n (4.17, 3.85, 3.12, and 0.26 for Ku, Kd, Zu, and Zd correspondingly) gives a quantitative basis for the evaluation of environmental impact of HPPs. Graphical abstract .

Effects of changing climate on European stream invertebrate communities: A long-term data analysis.

Long-term observations on riverine benthic invertebrate communities enable assessments of the potential impacts of global change on stream ecosystems. Besides increasing average temperatures, many studies predict greater temperature extremes and intense precipitation events as a consequence of climate change. In this study we examined long-term observation data (10-32years) of 26 streams and rivers from four ecoregions in the European Long-Term Ecological Research (LTER) network, to investigate invertebrate community responses to changing climatic conditions. We used functional trait and multi-taxonomic analyses and combined examinations of general long-term changes in communities with detailed analyses of the impact of different climatic drivers (i.e., various temperature and precipitation variables) by focusing on the response of communities to climatic conditions of the previous year. Taxa and ecoregions differed substantially in their response to climate change conditions. We did not observe any trend of changes in total taxonomic richness or overall abundance over time or with increasing temperatures, which reflects a compensatory turnover in the composition of communities; sensitive Plecoptera decreased in response to warmer years and Ephemeroptera increased in northern regions. Invasive species increased with an increasing number of extreme days which also caused an apparent upstream community movement. The observed changes in functional feeding group diversity indicate that climate change may be associated with changes in trophic interactions within aquatic food webs. These findings highlight the vulnerability of riverine ecosystems to climate change and emphasize the need to further explore the interactive effects of climate change variables with other local stressors to develop appropriate conservation measures.

Chemical Composition Analysis, Antimicrobial Activity and Cytotoxicity Screening of Moss Extracts (Moss Phytochemistry).

Mosses have been neglected as a study subject for a long time. Recent research shows that mosses contain remarkable and unique substances with high biological activity. The aim of this study, accordingly, was to analyze the composition of mosses and to screen their antimicrobial and anticancer activity. The total concentration of polyphenols and carbohydrates, the amount of dry residue and the radical scavenging activity were determined for a preliminary evaluation of the chemical composition of moss extracts. In order to analyze and identify the substances present in mosses, two types of extrahents (chloroform, ethanol) and the GC/MS and LC-TOF-MS methods were used. The antimicrobial activity was tested on four bacteria strains, and the anticancer activity on six cancer cell lines. The obtained results show the presence of a high number of primary (fatty acids and amino acids), but mainly secondary metabolites in moss extracts-including, sterols, terpenoids, polyphenols and others-and a high activity with respect to the studied test organisms.

Trends of natural organic matter concentrations in river waters of Latvia.

This study revealed significant (P < 0.1 and P < 0.05) increasing trends of total organic carbon (TOC) and water colour in most of the studied Latvian rivers during the last decade. However, over longer time periods, there were pronounced oscillations of TOC concentrations, similar to patterns of long-term changes of river discharge regime. On a yearly basis, there was a positive correlation between parameters of organic matter concentration and discharge in all selected rivers (r (S) = 0.540-0.803; P < 0.01). The impact of discharge on concentrations of organic matter could be masked by other factors, such as changes in precipitation, biological processes, soil types or land use.

The search for reference conditions for stream vegetation in northern Europe.

1. The European Water Framework Directive provides a framework for improving the ecological quality of stream ecosystems, with deviation from reference used as a measure of ecological status. 2. Here we examine the possibility of using less impacted stream sites from Latvia, Lithuania and Poland to establish a Danish reference network for macrophyte assemblages, and as a guiding image for identification of possible references sites within Denmark. Both approaches were evaluated using historical Danish records. 3. Four different macrophyte assemblages were identified for mid-sized streams in the Central and Eastern Lowland ecoregions. Macrophyte assemblages could not be delineated using physical stream site characteristics; however a gradual change in assemblage composition was attributed to differences in alkalinity and human impact. 4. Assemblages of contemporary vegetation in Denmark were quite similar to those found in Polish, Latvian and Lithuanian streams (26-35%). However, more importantly, from species-based predictions we noted higher similarity, particularly with Latvian and Lithuanian streams, before intensive land use commenced in Denmark (c. 1900). These results show that stream sites from these three countries can be considered in a Danish reference network. 5. Two of the four macrophyte assemblages comprised species such as Fontinalis antipyretica, Myriophyllum spicatum, Nuphar lutea, Potamogeton alpinus and P. perfoliatus that have a very scattered occurrence in the contemporary vegetation in Denmark. These groups were closely associated with the predictions from historic records, thereby lending support the conjecture that these assemblages could be part of the guiding image for the identification of potential reference sites within Denmark.