RESUMO
The content and spectrum of free fatty acids were studied in the cerebral white matter, on Wistar rats exposed to acute hypoxia (2% of oxygen in a respiratory mixture) for 3 min. The total content of fatty acids, especially of tetraenoic ones, rose sharply already 4 min after hypoxia and persisted elevated even 2 months after the hypoxia. The results showed that the degradation of phospholipids-containing biological membranes in the nervous tissue of the white matter starts immediately after hypoxia, and is continued for months.
Assuntos
Química Encefálica , Ácidos Graxos não Esterificados/análise , Hipóxia Encefálica/metabolismo , Animais , Cromatografia Gasosa , Ácidos Graxos não Esterificados/metabolismo , Ratos , Ratos EndogâmicosRESUMO
Diabetes-prone BioBreeding/Worcester (BB/Wor) rats received thrice weekly injections of mAb against antigens expressed on the surface of all T cells (OX19), cytotoxic/suppressor, and NK cells (OX8), helper/inducer cells (W3/25, OX35, OX38), and Ia+ cells (OX6, 3JP, OX17). Treatment with OX8 or OX19 achieved stable reductions of splenic and peripheral blood NK cells and helper/inducer T lymphocytes, respectively, and protected against diabetes. OX19 injections also prevented lymphocytic insulitis, thyroiditis, and the synthesis of autoantibodies to thyroid colloid and smooth muscle antigens. OX8 injections reduced splenic NK-mediated YAC-1 cell lysis, but did not prevent insulitis, thyroiditis, or autoantibody synthesis. Injections of mAb specific for antigens on the surface of helper/inducer cells, and for cells expressing IaE antigens provided marginal protection against diabetes without reductions of phenotypic subsets. These findings suggest that pancreatic beta cell destruction in the spontaneously diabetic BB/Wor rat is mediated by the combined action of NK and helper/inducer cells.
