RESUMO
Neurography and EMG are complementary techniques used in the diagnosis and monitoring of neuropathies. Both assess function of the peripheral nervous system and provide clinically useful information regarding the functional status of peripheral nerves. This information is not readily obtainable using biochemical, genetic or imaging techniques. I will discuss the role of these techniques in the diagnosis and management of neuropathies and some limitations of these techniques. These methods are routinely used in an EMG lab. These are most useful when used in conjunction with clinical examination to answer a well-defined clinical question. Reference values are required for interpretation of the data.
Assuntos
Eletrodiagnóstico/métodos , Eletromiografia/métodos , Doenças do Sistema Nervoso Periférico/diagnóstico , Doenças do Sistema Nervoso Periférico/terapia , Doenças Desmielinizantes/diagnóstico , Eletrodiagnóstico/instrumentação , Eletromiografia/instrumentação , Humanos , Doenças do Sistema Nervoso Periférico/genéticaRESUMO
OBJECTIVE: To report follow-up data in the evaluation of peripheral neuropathy in a 29-year old female after accidental deep hypothermia (13.7°C) in 1999. METHODS: Nerve conduction studies (NCS) and electromyography (EMG) were performed 20 days after the accident and again after 5 months and 1, 3, 5 and 11 years. Macro EMG was performed after 3, 5 and 11 years. To evaluate small fiber function, RR-interval, sympathetic skin response, quantitative sensory testing and skin biopsy for quantification of intra-epidermal nerve fiber density were performed in 2009. RESULTS: In the intensive care unit sensory and motor responses were absent except for the tibial nerves, and EMG showed profuse denervation. Improvement of amplitudes and conduction velocities was seen during the first 5 years. Muscular atrophy of hand muscles persisted. Large fibers were involved more extensively than small fibers. CONCLUSIONS: A severe axonal sensorimotor polyneuropathy developed in the intensive care unit following severe hypothermia. The mechanism was most likely cold injury to peripheral nerves. SIGNIFICANCE: The clinical picture and the laboratory findings indicate that even multi-organ dysfunction and, of specific interest in this study, a severe axonal degeneration may come to a good restitution after long time.
Assuntos
Hipotermia/complicações , Condução Nervosa/fisiologia , Doenças do Sistema Nervoso Periférico/fisiopatologia , Adulto , Eletromiografia/métodos , Feminino , Seguimentos , Mãos/fisiopatologia , Humanos , Atrofia Muscular/patologia , Atrofia Muscular/fisiopatologia , Doenças do Sistema Nervoso Periférico/etiologia , Nervo Tibial/fisiopatologiaRESUMO
OBJECTIVE: To create a reference value database for F wave parameters from healthy subjects aged 3-20 yr. METHODS: We studied the following parameters: minimum F wave latency minus distal motor latency (FMINLAT), number of F waves/20 stimuli (FNUMBER) and F wave dispersion (FDISP). The median, ulnar, peroneal and tibial nerves were studied. All four nerves were not analyzed in all subjects, the number of subjects varied from 78 to 118 in each nerve. RESULTS: Age explained 71-87% of the variability of FLATMIN while height explained 80-95% of the variability. The FMINLAT increases by 0.12 ms/cm of height in the upper limb nerves and by 0.28 ms/cm in the lower limb nerves. Gender did not influence the FMINLAT. FDISP was not related with age, height or gender. FNUMBER was not related with age or height, it was somewhat larger in males than females but the difference was not significant in all nerves. CONCLUSIONS: The best model for FMINLAT was a linear regression model with height as an independent variable. FDISP and FNUMBER are not related to age, height or gender between the ages of 3 and 20 yr. SIGNIFICANCE: We have constructed clinically useful reference values for F wave parameters in healthy subjects aged 3-20 yr for the main motor nerves commonly studied.
Assuntos
Potenciais de Ação/fisiologia , Eletrofisiologia/métodos , Potenciais Evocados/fisiologia , Neurônios Motores/fisiologia , Condução Nervosa/fisiologia , Nervos Periféricos/fisiologia , Adolescente , Criança , Pré-Escolar , Estimulação Elétrica/métodos , Feminino , Humanos , Masculino , Valores de Referência , Caracteres Sexuais , Adulto JovemRESUMO
In seronegative myasthenia gravis repetitive nerve stimulation and single-fibre EMG have a crucial diagnostic value but they may be negative, particularly in repetitive nerve stimulation studies. We report the case of a 43-year-old patient with generalized seronegative myasthenia gravis with negative 3 Hz repetitive nerve stimulation at Erb's point and voluntary single-fibre EMG in the orbicularis oculi. We also performed 6 and 12 Hz repetitive nerve stimulation at Erb and stimulated single-fibre EMG in the extensor digitorum communis and our findings were pathological. Our data suggest that, for individual patients with an atypical picture characterised by dissociation between a severe clinical pattern and no definite neurophysiological findings on conventional tests, repetitive nerve stimulation with a stimulation rate higher than 3 Hz and/or stimulated single-fibre EMG with an increasing stimulation rate may be helpful.
Assuntos
Eletromiografia/métodos , Músculo Esquelético/fisiopatologia , Miastenia Gravis/diagnóstico , Miastenia Gravis/fisiopatologia , Estimulação Elétrica Nervosa Transcutânea/métodos , Adulto , Humanos , Masculino , Miastenia Gravis/sangueRESUMO
Disorders affecting the postsynaptic side of the neuromuscular junction include autoimmune myasthenia gravis (MG) as well as some of the congenital myasthenic syndromes (CMS). Lambert-Eaton myasthenic syndrome (LEMS) is an acquired autoimmune neuromuscular disorder in which autoantibodies are directed against the presynaptic calcium channels. Here we describe two monozygous twin brothers: case 1 was diagnosed with an indeterminate form of acquired postsynaptic neuromuscular junction defect at age 32 and case 2 with LEMS at age 47. Case 1 presented clinically with mild generalized myasthenic weakness, neurophysiological examination revealed disturbed neuromuscular transmission along with probable myositis and serum analysis regarding antibodies against the acetylcholine receptor and muscle-specific tyrosine kinase was negative. Case 2 presented with proximal muscle fatigue accompanied by areflexia at rest and antibodies against the P/Q-type voltage-gated calcium channels were present. Neurophysiologically, case 2 had reduced baseline compound motor action potential amplitudes on neurography, decrement on low-frequency repetitive nerve stimulation (RNS) and pathological increment on high frequency RNS. To our knowledge this is the first case report of its kind and adds an intriguing contrast to the more common diagnosis of CMS in monozygous twins.
Assuntos
Doenças em Gêmeos , Síndrome Miastênica de Lambert-Eaton/fisiopatologia , Placa Motora/fisiopatologia , Doenças da Junção Neuromuscular/fisiopatologia , Adulto , Autoanticorpos/sangue , Autoanticorpos/imunologia , Autoantígenos/imunologia , Canais de Cálcio Tipo P/imunologia , Eletromiografia , Humanos , Imunossupressores/uso terapêutico , Síndrome Miastênica de Lambert-Eaton/diagnóstico , Síndrome Miastênica de Lambert-Eaton/genética , Síndrome Miastênica de Lambert-Eaton/imunologia , Masculino , Miosite/complicações , Doenças da Junção Neuromuscular/diagnóstico , Doenças da Junção Neuromuscular/genética , Doenças da Junção Neuromuscular/imunologia , Reflexo Anormal , Transmissão Sináptica , Gêmeos MonozigóticosRESUMO
AIMS: Elderly individuals and patients with polyneuropathy often feel heat pain or burning sensation on quantitative sensory testing (QST) of warm perception distally in the lower limbs. We therefore studied heat pain threshold (HPT), warm perception threshold (WPT) and the difference between heat pain and warm perception thresholds in 48 patients with symptoms and signs of polyneuropathy matched according to age and gender with 48 healthy persons. METHODS: QST (using method of limits) was performed on the distal calf and the dorsal foot. RESULTS: Particularly in the neuropathy group several individuals (58%) had an unpleasant feeling, often burning, when the thresholds according to the WPT algorithm were recorded. Difference between heat pain and warm perception thresholds in the lower calf of the patients was 3.9 +/- 3.5 and 5.8 +/- 3.4 degrees C in the controls (P = 0.012), and on the foot 3.8 +/- 2.8 vs 5.3 +/- 3.6 degrees C (P = 0.02). CONCLUSIONS: When performing QST it is important to assess also quality features of warm perception, such as burning and heat pain sensation.
Assuntos
Limiar da Dor/fisiologia , Polineuropatias/fisiopatologia , Adulto , Fatores Etários , Idoso , Estudos de Casos e Controles , Feminino , Pé/inervação , Temperatura Alta , Humanos , Perna (Membro)/inervação , Masculino , Pessoa de Meia-Idade , Condução Nervosa/fisiologia , Sensação Térmica/fisiologiaRESUMO
The aim of this study is to verify whether degeneration of skin receptors or intradermal nerve endings by topical application of capsaicin modifies the double peak response obtained by submaximal anodal stimulation. Five healthy volunteers topically applied capsaicin to the finger-tip of digit III (on the distal phalanx) four times daily for 4-5 weeks. Before and after local capsaicin applications, we studied the following electrophysiological findings: compound sensory action potential (CSAP), double peak response, sensory threshold and double peak stimulus intensity. Local capsaicin application causes disappearance or decrease of the second component of the double peak, which gradually increases after the suspension of capsaicin. Conversely, no significant differences were observed for CSAP, sensory threshold and double peak stimulus intensity. This study suggests that the second component of the double peak may be a diagnostic tool suitable to show an impairment of the extreme segments of sensory nerve fibres in distal sensory axonopathy in the early stages of damage, when receptors or skin nerve endings are impaired but undetectable by standard nerve conduction studies.
Assuntos
Capsaicina/administração & dosagem , Estimulação Elétrica , Potenciais Evocados/efeitos dos fármacos , Fármacos do Sistema Sensorial/administração & dosagem , Limiar Sensorial/efeitos dos fármacos , Administração Cutânea , Adulto , Análise de Variância , Relação Dose-Resposta à Radiação , Estimulação Elétrica/métodos , Potenciais Evocados/fisiologia , Potenciais Evocados/efeitos da radiação , Feminino , Humanos , Masculino , Terminações Nervosas/efeitos dos fármacos , Tempo de Reação/efeitos dos fármacos , Tempo de Reação/efeitos da radiação , Pele/inervaçãoRESUMO
Post-polio syndrome (PPS) is characterized by new or increased muscular weakness, atrophy, muscle pain and fatigue several years after acute polio. The aim of the article is to prepare diagnostic criteria for PPS, and to evaluate the existing evidence for therapeutic interventions. The Medline, EMBASE and ISI databases were searched. Consensus in the group was reached after discussion by e-mail. We recommend Halstead's definition of PPS from 1991 as diagnostic criteria. Supervised, aerobic muscular training, both isokinetic and isometric, is a safe and effective way to prevent further decline for patients with moderate weakness (Level B). Muscular training can also improve muscular fatigue, muscle weakness and pain. Training in a warm climate and non-swimming water exercises are particularly useful (Level B). Respiratory muscle training can improve pulmonary function. Recognition of respiratory impairment and early introduction of non-invasive ventilatory aids prevent or delay further respiratory decline and the need for invasive respiratory aid (Level C). Group training, regular follow-up and patient education are useful for the patients' mental status and well-being. Weight loss, adjustment and introduction of properly fitted assistive devices should be considered (good practice points). A small number of controlled studies of potential-specific treatments for PPS have been completed, but no definitive therapeutic effect has been reported for the agents evaluated (pyridostigmine, corticosteroids, amantadine). Future randomized trials should particularly address the treatment of pain, which is commonly reported by PPS patients. There is also a need for studies evaluating the long-term effects of muscular training.
Assuntos
Neurologia , Síndrome Pós-Poliomielite/diagnóstico , Síndrome Pós-Poliomielite/terapia , Guias de Prática Clínica como Assunto , Sociedades Médicas , Comitês Consultivos , Amantadina/uso terapêutico , Antivirais/uso terapêutico , Inibidores da Colinesterase/uso terapêutico , Europa (Continente) , Terapia por Exercício/métodos , Humanos , MEDLINE/estatística & dados numéricos , Imageamento por Ressonância Magnética , Neurofisiologia/métodos , Exame Físico , Síndrome Pós-Poliomielite/fisiopatologia , Esteroides/uso terapêuticoRESUMO
The aim of this study was to translate and validate the disease-specific patient-derived Myasthenia Gravis (MG) Questionnaire to enable use among Swedish MG patients. The original Italian version of the MG Questionnaire (MGQ) was translated into Swedish and transculturally adapted. The validity and reliability was tested on 48 Swedish MG patients. We correlated MGQ scores with disease severity and with the Swedish version of the Short-Form 36-item general health survey (SF-36). Reproducibility was assessed on 18 clinically stable MG patients. A significant correlation regarding the MGQ scores was seen when correlated with physical scores of the SF-36 and the overall clinical status. Internal consistency and reproducibility was excellent. We conclude that the evaluation capacities of the Swedish MGQ are equivalent to those of the original Italian version of the MGQ. The questionnaire was successfully validated as an outcome measure also for Swedish MG patients, which is important for international multicentre clinical trials.
Assuntos
Miastenia Gravis , Avaliação de Resultados em Cuidados de Saúde , Inquéritos e Questionários , Adulto , Idoso , Feminino , Inquéritos Epidemiológicos , Humanos , Masculino , Pessoa de Meia-Idade , Reprodutibilidade dos Testes , Suécia , TraduçõesRESUMO
OBJECTIVE: To compare the electrophysiological and histopathological features of immunological myasthenia gravis (MG) subtypes. METHODS: Fifty MG patients underwent clinical examination, MuSK-Ab and AChR-Ab analysis. The majority underwent quantitative and single-fiber electromyography (QEMG, SFEMG), repetitive nerve stimulation and deltoid muscle biopsy. From muscle specimens with histological mitochondrial dysfunction, we amplified mitochondrial DNA (mtDNA). In specimens with mtDNA deletions, the nuclear gene POLG1 was sequenced. RESULTS: Five AChR-Ab seropositive [AChR(+)] and 5 seronegative [AChR(-)] patients were MuSK-Ab seropositive [MuSK(+)]. Five of 7 neurophysiologically examined MuSK(+) patients (71%) had proximal myopathic pattern, compared to 7 of 31 MuSK(-)/AChR(+) patients (23%) (P=0.012). SFEMG was abnormal in all examined MuSK(+) patients. All 7 biopsied MuSK(+) and 32 MuSK(-) patients (89%) had cytochrome c oxidase (COX) negative fibers. Three of five MuSK(+) and 13 of 20 MuSK(-) patients analyzed had multiple mtDNA deletions but no POLG1 mutations. CONCLUSIONS: Similar degree of SFEMG abnormalities was present in proximal muscles among MuSK(+) and AChR(+) patients. Proximal myopathy was over-represented in MuSK(+) patients; however, both MuSK(+) and MuSK(-) patients had mild myopathy with frequent mitochondrial abnormalities. SIGNIFICANCE: The weakness in MuSK(+) patients is most likely due to disturbed neuromuscular transmission. The frequently encountered mitochondrial dysfunction in MG warrants further study.
Assuntos
Mitocôndrias Musculares/patologia , Músculo Esquelético , Miastenia Gravis , Receptores Proteína Tirosina Quinases/imunologia , Receptores Colinérgicos/imunologia , Potenciais de Ação/fisiologia , Adulto , Idoso , Anticorpos/metabolismo , Estudos de Casos e Controles , DNA Mitocondrial/genética , Estimulação Elétrica/métodos , Eletromiografia/métodos , Complexo IV da Cadeia de Transporte de Elétrons/metabolismo , Feminino , Humanos , Imunoglobulinas/metabolismo , Masculino , Pessoa de Meia-Idade , Mitocôndrias Musculares/imunologia , Fibras Musculares Esqueléticas/metabolismo , Fibras Musculares Esqueléticas/patologia , Fibras Musculares Esqueléticas/fisiologia , Músculo Esquelético/patologia , Músculo Esquelético/fisiopatologia , Miastenia Gravis/imunologia , Miastenia Gravis/patologia , Miastenia Gravis/fisiopatologia , Estudos Prospectivos , Reação em Cadeia da Polimerase Via Transcriptase Reversa/métodosRESUMO
Small diameter nerve fibre (SDNF) neuropathy is an axonal sensory neuropathy affecting unmyelinated (C) and thin myelinated (A-delta) fibres. We have evaluated 75 patients with symptoms and signs suggesting SDNF dysfunction with or without symptoms and signs of co-existing large diameter nerve fibre involvement. The patients were examined clinically and underwent skin biopsy, quantitative sensory testing (QST) and nerve conduction studies (NCS). The purpose of this study was to compare the relationship between the different methods and in particular measurements of thermal thresholds and intraepidermal nerve fibre (IENF) density in the same site of the distal leg. The main subdivision of the patient material was made according to the overall NCS pattern. Patients with normal NCS (38) had 6.4 +/- 3.8 and patients with abnormal NCS (37) had 4.4 +/- 3.4 IENF per mm (P = 0.02). Limen (difference between warm and cold perception thresholds) was significantly higher (more abnormal) in those with abnormal than in those with normal NCS (22.1 +/- 9.1 vs. 13.4 +/- 5.6, P < 0.0001). Cold perception threshold was more abnormal (P < 0.0001) than warm perception threshold (P = 0.002). Correlation between IENF and QST was statistically significant only when NCS was abnormal, and thus dependent of a more severe neuropathic process in SDNFs.
Assuntos
Fibras Nervosas/fisiologia , Condução Nervosa/fisiologia , Doenças do Sistema Nervoso Periférico/patologia , Doenças do Sistema Nervoso Periférico/fisiopatologia , Limiar Sensorial/fisiologia , Pele/inervação , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Biópsia/métodos , Criança , Estimulação Elétrica/métodos , Feminino , Humanos , Modelos Lineares , Masculino , Pessoa de Meia-Idade , Fibras Nervosas/patologia , Doenças do Sistema Nervoso Periférico/classificação , Tempo de Reação/fisiologia , Tempo de Reação/efeitos da radiação , Estudos Retrospectivos , Pele/patologia , Sensação Térmica/fisiologiaRESUMO
Here we report how the different types of regional muscle involvement, i.e. bulbar, ocular or generalized, in patients with myasthenia gravis (MG) influence the mental aspects of quality of life. Clinical examination according to Osserman was performed in 48 MG patients (45 women, three men; mean age 54, SD 12 years). Each patient was at the time for clinical evaluation asked to fill out the disease-specific Myasthenia Gravis Questionnaire (MGQ) and the Short-Form 36-item questionnaire for health survey (SF-36) as patient-oriented tools. We related the regional domains (generalized domain, bulbar domain and ocular domain) of the MGQ and the clinical findings, respectively, with mental quality of life as assessed by SF-36. Bulbar and generalized involvement results in impairment of mental aspects of quality of life, whereas ocular involvement does not.
Assuntos
Debilidade Muscular/fisiopatologia , Miastenia Gravis/fisiopatologia , Miastenia Gravis/psicologia , Qualidade de Vida , Estatística como Assunto , Adulto , Feminino , Indicadores Básicos de Saúde , Humanos , Masculino , Pessoa de Meia-Idade , Estudos Prospectivos , Índice de Gravidade de Doença , Inquéritos e QuestionáriosRESUMO
OBJECTIVE: To determine the source of an abnormal pattern of latency shifts leading to falsely high jitters in single fibre electromyography (SFEMG). METHODS: We observed a sudden shortening of the latency to an individual single fibre spike component followed by a gradual return to baseline values during stimulation single fibre electromyography (SFEMG) of the facial muscle. The pattern could be reproduced in healthy controls. RESULTS: The sudden decrease in latency proved to follow an additional discharge of the muscle fibre, not due to the external stimulus. This additional discharge was identified as an F-response. CONCLUSIONS: The mechanism is thought to be a higher muscle fibre conduction velocity resulting from a temporary increase in stimulus frequency, in the form of an extra impulse along the muscle fibre represented by the F-response. SIGNIFICANCE: The typical abnormal pattern should be recognised because it can falsely increase the mean jitter. We advice to increase the time base to 50 ms if this pattern is observed and to exclude the affected potentials from jitter measurements.
Assuntos
Eletromiografia , Músculos Faciais/fisiopatologia , Tempo de Reação/efeitos da radiação , Algoritmos , Estimulação Elétrica/métodos , Músculos Faciais/efeitos da radiação , Humanos , Doenças Neuromusculares/fisiopatologia , Fatores de TempoRESUMO
OBJECTIVE: The aim of these modeling studies on the generation of motor unit potentials (MUPs) was to establish the influence of MU parameters and recording conditions on the shape irregularity of MUPs. The focus was on the dependence of the irregularity of MUPs on fiber density, fiber diameters and the recording distance from the end-plate zone. METHODS: The study was performed using the 'EMG Simulator' software for modeling of MUP and our own software for calculations. RESULTS: The results indicate that the irregularity of a MUP increases with increased recording distance from the end-plate zone and decreases with increased fiber diameter and/or with increased fiber density. The quantitative relationship between these factors has been derived. CONCLUSIONS: The relationships determine the structural conditions in which irregular potential may be generated and therefore may be helpful in the interpretation of atypical MUPs.
Assuntos
Modelos Neurológicos , Fibras Musculares Esqueléticas/fisiologia , Músculo Esquelético/fisiologia , Junção Neuromuscular/fisiologia , Potenciais de Ação , Simulação por Computador , Eletrodos , Eletromiografia/instrumentação , Humanos , Placa Motora/fisiologia , Fibras Musculares Esqueléticas/ultraestrutura , Músculo Esquelético/inervação , Músculo Esquelético/ultraestruturaRESUMO
OBJECTIVE: In this paper we report a painful nondystrophic myotonia which has not been previously described. Pain is a rare symptom in myotonia. We report a myotonic disorder in a 34-year-old woman and her 14-year-old daughter. Painful cramps occur during and after exercise in the mother, and both patients can demonstrate unusual contractions in the tongue. In the present study we try to evaluate the mechanisms behind the unique finding of trains of high amplitude of positive waves, not seen in the earlier known myotonic conditions. METHODS: Clinical investigations and electromyography with single and dual channel recordings and muscle morphometry were performed. RESULTS: The electromyographic recordings reveal positive waves, fibrillation potentials and myotonic discharges. In addition, extraordinary findings were made of trains of high frequency positive potentials with very high amplitudes and with conduction block along the muscle fibres. CONCLUSIONS: In this new form of myotonia with likely dominant heredity, the specific finding of trains of high amplitude positive waves indicates ephaptic transmission within bundles of neighbouring muscle fibres.
Assuntos
Miotonia/complicações , Miotonia/fisiopatologia , Dor/etiologia , Dor/fisiopatologia , Língua/fisiopatologia , Adolescente , Adulto , Eletromiografia , Feminino , Humanos , Cãibra Muscular/etiologia , Cãibra Muscular/genética , Cãibra Muscular/fisiopatologia , Miotonia/genética , Dor/genética , Linhagem , Língua/inervaçãoRESUMO
Herpes zoster (HZ) is essentially a viral disease of the posterior root ganglia and sensory nerve fibers, which presents clinically with vesicular eruption of the skin, radicular pain and sensory changes in the distribution of the affected ganglion. However, motor involvement can be seen as well. If classic cutaneous lesions are present, HZ-related motor paresis is easily diagnosed. Otherwise, the diagnosis may be suspicious, especially if the weakness occurs before the cutaneous lesions have appeared, or weeks after they have subsided. We present a patient with HZ-related motor paresis due to radiculopathy in the cervical segments whose motor symptoms and signs appear as major clinical features.
Assuntos
Herpes Zoster/complicações , Herpes Zoster/diagnóstico , Paresia/diagnóstico , Paresia/etiologia , Idoso , Idoso de 80 Anos ou mais , Herpes Zoster/terapia , Humanos , Masculino , Paresia/terapiaRESUMO
The possibility that antiserum to tumour necrosis factor-alpha (TNF-alpha) is neuroprotective in spinal cord injury (SCI) was examined in a rat model. SCI was produced by making an incision into the right dorsal horn at the T10-11 segments. Top TNF-alpha antiserum at three concentrations (1:10; 1:50 and 1:100) given 30 min before or 2 min, 5 min or 10 min after trauma resulted in marked reduction in visible swelling, edema formation, and leakage of radiolabelled iodine tracer within the T9 and T12 segments at 5 h in a dose dependent manner. This neuroprotective effect was most pronounced when the antiserum at the highest dose level (1:10) was applied 10 min after SCI. The TNF-alpha antiserum also reduced the SCI induced upregulation of neuronal nitric oxide synthase (nNOS) immunoreactivity in a concentration dependent manner. Taken together, these results suggest that local application of TNF-alpha antiserum is neuroprotective in SCI and that this effect is mediated through NOS regulation.
Assuntos
Edema/prevenção & controle , Soros Imunes/administração & dosagem , Doenças da Medula Espinal/prevenção & controle , Traumatismos da Medula Espinal/fisiopatologia , Fator de Necrose Tumoral alfa/imunologia , Administração Tópica , Animais , Permeabilidade Capilar/efeitos dos fármacos , Edema/etiologia , Imuno-Histoquímica , Masculino , Óxido Nítrico Sintase/metabolismo , Ratos , Ratos Sprague-Dawley , Medula Espinal/irrigação sanguínea , Doenças da Medula Espinal/etiologia , Traumatismos da Medula Espinal/complicações , Traumatismos da Medula Espinal/patologiaRESUMO
The influence of the potent L-type Ca[2+] channel antagonist Nimodipine on spinal cord evoked potentials (SCEP) and axonal injury following trauma to the spinal cord was examined in a rat model. Spinal cord injury (SCI) was produced by an incision into the right dorsal horn of the T10-11 segments under urethane anaesthesia (1.5 g/kg, i.p.). SCEPs were recorded by epidural electrodes placed over the T9 (rostral) and T12 (caudal) segments after stimulation of the right tibial and sural nerves. SCI induced a pronounced decrease of the SCEP negative amplitude in the rostral (T9) recordings immediately after trauma. Axonal injury seen as degradation of myelin basic protein (MBP) immunostaining and myelin vesiculation at the ultrastructural level was most pronounced at 5 h. Continuous administration of Nimodipine (2 microg/kg/min, i.v.) from 30 min prior to injury until sacrifice markedly attenuated the changes in SCEP amplitude and latency. Axonal damage, loss of MBP, and myelin vesiculation were much less evident in the nimodipine treated traumatised rats. These observations suggest that Ca[2+] channels play an important role in the trauma induced alterations in SCEP and axonal injury, and indicate a therapeutic value of Ca[2+] blockers in SCI.
Assuntos
Bloqueadores dos Canais de Cálcio/farmacologia , Canais de Cálcio Tipo L/efeitos dos fármacos , Lesão Axonal Difusa/etiologia , Condução Nervosa/efeitos dos fármacos , Nimodipina/farmacologia , Traumatismos da Medula Espinal/complicações , Traumatismos da Medula Espinal/fisiopatologia , Animais , Lesão Axonal Difusa/patologia , Edema/prevenção & controle , Potenciais Evocados/efeitos dos fármacos , Masculino , Bainha de Mielina/efeitos dos fármacos , Bainha de Mielina/ultraestrutura , Ratos , Ratos Sprague-Dawley , Tempo de Reação/efeitos dos fármacos , Fluxo Sanguíneo Regional/efeitos dos fármacos , Medula Espinal/irrigação sanguínea , Medula Espinal/patologia , Medula Espinal/fisiopatologia , Doenças da Medula Espinal/prevenção & controle , Traumatismos da Medula Espinal/patologiaRESUMO
Dynorphin is a neuropeptide that is present in high quantities in the dorsal horn of the spinal cord. The peptide is actively involved in pain processing pathways. However, its involvement in spinal cord injury is not well known. Alteration in dynorphin immunoreactivity occurs following a focal trauma to the rat spinal cord. Infusion of dynorphin into the intrathecal space of the cord results in ischemia, cell damage and abnormal motor function. Antibodies to dynorphin when injected into the intrathecal space of the spinal cord following trauma improve motor recovery, reduce edema and cell changes. However, influence of dynorphin on trauma induced alteration in spinal cord bioelectrical activity is still not known. Spinal cord evoked potentials (SCEP) are good indicator of spinal cord pathology following trauma. Therefore, in present investigation, influence of dynorphin antibodies on trauma induced changes in SCEP were examined in our rat model. In addition, spinal cord edema formation, microvascular permeability disturbances and cell injury were also investigated. Our results show that topical application of dynorphin antiserum (1 : 200) two min before injury markedly attenuated the SCEP changes immediately after injury. In the antiserum treated animals, a significant reduction in the microvascular permeability, edema formation and cell injury was observed in the traumatised spinal cord. These observations suggest that (i). dynorphin is involved in the altered bioelectrical activity of the spinal cord following trauma, (ii). the peptide actively participates in the pathophysiological processes of cell injury in the spinal cord trauma, and (iii). the dynorphin antiserum has potential therapeutic value for the treatment of spinal cord injuries.