RESUMO
Spontaneously hypertensive rats (SHR) display several features of the human insulin-resistance syndromes. Cd36 deficiency is genetically linked to insulin resistance in SHR. We show that transgenic expression of Cd36 in SHR ameliorates insulin resistance and lowers serum fatty acids. Our results provide direct evidence that Cd36 deficiency can promote defective insulin action and disordered fatty-acid metabolism in spontaneous hypertension.
Assuntos
Antígenos CD36/genética , Hipertensão/genética , Resistência à Insulina/genética , Animais , Animais Geneticamente Modificados , Antígenos CD36/biossíntese , Ácidos Graxos/sangue , Teste de Tolerância a Glucose , Ratos , Ratos Endogâmicos SHRRESUMO
To investigate whether a BP-regulatory locus exists in the vicinity of the renin locus on rat chromosome 13, we transferred this chromosome segment from the Dahl salt-sensitive (S) rat onto the genetic background of the Dahl salt-resistant (R) rat. In congenic Dahl R rats carrying the S renin gene and fed an 8% salt diet, systolic BP was significantly lower than in progenitor Dahl R rats: 127 +/- 1 mmHg versus 138 +/- 4 mmHg, respectively (P < 0.05). Moreover, the decreased BP in the congenic Dahl R strain was associated with decreased kidney renin mRNA and decreased plasma renin concentration. These findings demonstrate that the Dahl S strain carries alleles in or near the renin locus that confer lower plasma renin concentration and lower BP than the corresponding alleles in the Dahl R strain, at least when studied on the genetic background of the Dahl R rat and in the environment of a high salt diet. The occurrence of coincident reductions in kidney renin mRNA, plasma renin concentration, and BP after interstrain transfer of naturally occurring renin gene variants strongly suggests that genetically determined variation in renin gene expression can affect BP.
Assuntos
Hipertensão/genética , Renina/genética , Alelos , Animais , Mapeamento Cromossômico , Dieta , Feminino , Regulação Enzimológica da Expressão Gênica , Marcadores Genéticos , Rim/enzimologia , Masculino , RNA Mensageiro/genética , Ratos , Ratos Endogâmicos , Ratos Mutantes , Renina/administração & dosagem , Cloreto de Sódio na DietaRESUMO
The Dahl salt-sensitive rat (SS/Jr) is a widely used animal model of salt-sensitive hypertension. SS/Jr rats are believed to be highly inbred and uniformly sensitive to the hypertensinogenic effects of sodium chloride, but we have recently observed that SS/Jr rats from Harlan Sprague Dawley, Inc, exhibit considerable variability in their blood pressure response to supplemental dietary salt. To test the possibility that commercially available SS/Jr rats are genetically contaminated and therefore no longer fully inbred, we performed molecular genetic studies and blood pressure measurements in several groups of SS/Jr rats purchased from Harlan Sprague Dawley. We found molecular evidence of heterozygosity and/or atypical allelic variants involving loci on at least five different chromosomes. Many of the rats also failed to exhibit a salt-sensitive blood pressure phenotype. We conclude that SS/Jr rats being sold by the only commercial vendor of Dahl rats in the United States are genetically contaminated and resistant to the hypertensinogenic effects of salt. These findings raise serious questions about the interpretation of research conducted with SS/Jr rats obtained from Harlan Sprague Dawley.
Assuntos
DNA Satélite/análise , Hipertensão/genética , Ratos Mutantes/genética , Cloreto de Sódio/farmacologia , Animais , Sequência de Bases , Pressão Sanguínea/efeitos dos fármacos , Resistência a Medicamentos/genética , Feminino , Hipertensão/induzido quimicamente , Masculino , Sondas Moleculares/genética , Dados de Sequência Molecular , Reação em Cadeia da Polimerase , Ratos , Ratos Mutantes/fisiologiaAssuntos
Fosfatase Alcalina/genética , Cristalinas/genética , Genes , Inibinas/genética , Antígenos Comuns de Leucócito/genética , Ratos/genética , Renina/genética , ATPase Trocadora de Sódio-Potássio/genética , Animais , Mapeamento Cromossômico , Cruzamentos Genéticos , Humanos , Escore Lod , Camundongos/genética , Proteína Fosfatase 1 , Ratos Endogâmicos Lew , Ratos Endogâmicos SHR , Ratos Endogâmicos , Especificidade da EspécieRESUMO
In the rat, the results of genetic linkage studies by "candidate" gene or "positional mapping" approaches have suggested that DNA sequences that regulate blood pressure may be located in the vicinity of the kallikrein gene family on chromosome 1, the gene for angiotensin-converting enzyme on chromosome 10, the renin gene on chromosome 13, and the major histocompatibility complex on chromosome 20. Some studies have also suggested that blood pressure regulatory genes may be located on the sex chromosomes. Pending the results of confirmatory studies, these experiments should be interpreted with caution. However, with confirmation of these studies, it should be possible to create a variety of new animal models that will provide excellent opportunities for investigating the molecular, biochemical, and physiologic determinants of high blood pressure. In addition, in genetic studies in humans with essential hypertension, it may be worthwhile to target chromosome regions that are homologous to those implicated in linkage studies of hypertension in rodents. By narrowing the focus on selected areas of the genome, experimental linkage studies in the rat may also be used to guide the detailed molecular approaches ultimately required to identify the specific DNA sequence alterations that give rise to increased blood pressure.
