[Serum concentration of selected adhesive molecules in early localised and late disseminated Lyme borreliosis]. / Stezenia rozpuszczalnych form wybranych czasteczek adhezyjnych we wczesnej zlokalizowanej oraz póznej rozsianej boreliozie z Lyme.

Serum concentration of soluble form ICAM-1, PECAM-1, VAP-1, E-selectin, VCAM-1 in 20 patients with Erythema migrans and 20 patients with Lyme arthritis before and after 4-weeks antibiotic treatment were estimated. Comparing group consited 8 healthy volunteers. Concentrations of soluble molecules were performed with ELISA kits(Bender MedSystem, Austria). Results of the study indicate involvment of soluble form ICAM-1, PECAM-1, VAP-1, E-selectin and VCAM-1 in immunopathogenesis of Lyme borreliosis, and different engagement of molecules in depend on stage of disease. In early localized stage of disease, results suggest participation molecules engaged in all stages of "adhesion cascade" whilst in disseminated, late stage of Lyme borreliosis molecules of tight adhesion. The measurement of soluble form od aadhesion molecules can be useful of in differentiation of localized and disseminated late stages of Lyme borreliosis helpful in clinical diagnosis.

[Comparison of test with antigen VlsE (C6) with tests with recombinant antigens in patients with Lyme borreliosis]. / Porównanie wyników testu z antygenem VIsE (C6) oraz testów z zastosowaniem antygenów rekombinowanych u chorych na borelioze z Lyme.

UNLABELLED: In diagnosis of Lyme borreliosis classic, recombinant antigens are used. Introduced recombinant antigen VlsE increases hope to improve sensibility of the tests. AIM OF THE STUDY: Serological results detecting antibodies against Borrelia burgdorferi, recombinant antigens in both classes and test with VlsE were compared. MATERIAL AND METHODS: Quick ELISA C6 Borrelia Assay Kit (U.S.A.) for both classes simultaneusly were compared with two tests composed of recombinant antigens Borrelia IgG recombinant, Borrelia IgM recombinant (Biomedica, Austria)separate for IgM i IgG antibodies. Patients with borreliosis as eryhtema migrans were evaluated (n=36) and group with chronic Lyme arthritis (n=68). RESULTS: Statistical coincidence between results of test ELISA C6 and presence of antibodies IgM (p < 0.0001) and IgG (p < 0.0001) obtained in tests based on recombinant antigens in group erytema migrans. In group Lyme arthritis coincidence (p < 0.0002), with IgM antibodies was stated. CONCLUSIONS: Test ELISA C6 (with protein VlsE-C6) in group of patients with Lyme borreliosis had statistically essential predictive values of presence antibodies in IgM i IgG class, stated in tests performed separately for IgM and IgG classes with recombinant antigens. Tests based on conservative part of protein VlsE (C6), detecting antibodies in both classes are very promising in diagnosis of early stages Lyme borreliosis.