RESUMO
We have isolated the cDNA of avian Mox2 and analyzed its expression pattern during somitogenesis and limb bud formation. Mox2 plays an important role in limb muscle differentiation in the mouse. Mox2 is expressed in the somites of developing chick embryos and in presumptive migrating myoblasts from the dermomyotome to the limb buds. It is also expressed in the ventral and dorsal part of limb buds and is associated with non-proliferating myoblasts. Significant differences were observed in chick and mouse expression patterns, namely in the chick dermomyotome and limb.
Assuntos
Antígenos de Superfície/genética , Sequência de Aminoácidos , Animais , Antígenos CD , Bromodesoxiuridina/metabolismo , Embrião de Galinha , Clonagem Molecular , DNA Complementar/metabolismo , Proteínas de Ligação a DNA/biossíntese , Extremidades/embriologia , Humanos , Imuno-Histoquímica , Hibridização In Situ , Camundongos , Dados de Sequência Molecular , Fator de Transcrição PAX3 , Fatores de Transcrição Box Pareados , Ratos , Homologia de Sequência de Aminoácidos , Fatores de Tempo , Distribuição Tecidual , Fatores de Transcrição/biossínteseRESUMO
Mox1 and Mox2 homeobox genes have been shown to be critical in axial skeleton and in limb muscle development respectively. Pax1 and Pax3 gene products are also implicated in these processes. Mox and Pax expression patterns are highly overlapping both spatially and temporally during embryonic development. We show here for the first time that Mox proteins physically interact with Pax1 and Pax3 using the yeast two-hybrid protein interaction assay as well as in vitro biochemical assays. There is a strong preference of Mox1 to associate with Pax1 rather than Pax3 and of Mox2 to associate with Pax3 rather than Pax1. The observed interactions are mediated through the homeodomain of Mox.