Analysis of paraphenylenediamine.

The toxic Paraphenylenediamine is characterized by infrared spectrophotometry and nuclear magnetic resonance spectroscopy, whereas, high performance liquid chromatography with a refractive index detector was used to determine its purity in the suspect samples using external standardisation. An analytical method for determination of lower traces of paraphenylenediamine in post-mortem biological fluids was developed. This procedure involves deproteneization or hydrolysis followed by liquid-liquid extraction and derivatization with trifluoroacetic anhydride. 1 microL of the extract was then analysed by gas chromatography/iontrap mass spectrometry. Benzidine used as the internal standard for quantification and the extraction recovery test was evaluated to 85%. This method was validated in cases with paraphenylenediamine poisoning.

Headspace-GC/MS detection of TATP traces in post-explosion debris.

In this study, we describe a HS-GC/MS method as a new analytical technique for the detection of triacetone triperoxide (TATP) traces in samples from post-explosion debris. We evaluate the optimum parameters for the analysis of this product with lowliness thermal decomposition and for the detection of TATP lesser than nanogram (.1 ng) level.

Optimization of an analytical method for detecting paraphenylenediamine (PPD) by GC/MS-iontrap in biological liquids.

A method for qualitative and quantitative determination of paraphenylenediamine in biological fluids (blood, urine and gastric content) was developed. This analytical procedure involves deproteneisation or hydrolysis, liquid-liquid extraction, derivatization with TFA followed by GC-MS iontrap analysis. Benzidine was the internal standard used for quantification and the extraction recovery test was about 85%. The detection limit of paraphenylenediamine was determined at 0.1 pg (S/N=10).