RESUMO
The most common approaches for the in-situ bioremediation of contaminated sites worldwide are bioaugmentation and biostimulation. Biostimulation has often proved more effective for chronically contaminated sites. This study examined the effectiveness of optimized water hyacinth compost in comparison with other organic and inorganic amendments for the remediation of crude oil-polluted soils. Water hyacinth was found to be rich in nutrients necessary to stimulate microbial growth and activity. An organic geochemical analysis revealed that all amendments in this study increased total petroleum hydrocarbon (TPH) biodegradation by ≥75% within 56 days, with the greatest biodegradation (93%) occurring in sterilized soil inoculated with optimized water hyacinth compost. This was followed by polluted soil amended with a combination of spent mushroom and water hyacinth composts (SMC + WH), which recorded a TPH biodegradation of 89%. Soil amendment using the inorganic fertilizer NPK (20:10:10) resulted in 86% TPH biodegradation. On the other hand, control samples (natural attenuation) recorded only 4% degradation. A molecular analysis of residual polycyclic aromatic hydrocarbons (PAHs) showed that the 16 PAHs designated by the US EPA as priority pollutants were either completely or highly degraded in the combined treatment (SMC + WH), indicating the potential of this amendment for the environmental remediation of soils contaminated with recalcitrant organic pollutants.
RESUMO
Water hyacinth (Eichhornia crassipes) is a hydrophyte weed that causes havoc in the aquatic ecosystem as an invasive plant that can obstruct waterways and bring about nutrient imbalance. This study aims to address how this invasive hydrophyte can be physically harvested and biochemically transformed into a bioproduct that can enhance the restoration of damaged soil. Biocomposting, a low-cost biotechnological technique, was designed to degrade the lignocellulosic Eichhornia crassipes biomass and transform it into a valuable bioproduct. The process used response surface methodology (RSM) to investigate the aggregate effect of moisture content, turning frequency, and microbial isolate (Chitinophaga terrae) inoculum size on the breakdown of lignin over 21 days. The moisture content (A), (45, 55, 65) % v/w, inoculum size (B), (5, 7.5, 10)% v/v, and turning frequency (C), (1, 3, 5) days were considered independent variables, while percentage lignin degradation was considered a response variable. The optimal conditions for lignin breakdown were 65.7 percent (v/w) moisture, 7.5 percent (v/v) inoculum concentration, and 5-day interval turning. The R2 score of 0.9733 demonstrates the model's integrity and reliability. Thus, the RSM approach resulted in a fine grain dark brown Nutri-compost that proved effective in enhancing soil fertility. This procedure is recommended for a scale-up process where large quantities of the hydrophyte could be treated for conversion into Nutri compost.
