Stock structure of blue threadfin Eleutheronema tetradactylum across northern Australia, as indicated by parasites.

The parasite fauna of the blue threadfin Eleutheronema tetradactylum, collected from 14 sites across northern Australia, was examined to evaluate the degree of movement and subsequent stock structure of the fish. Univariate and multivariate analysis of nine 'permanent' parasite species [the nematodes Anisakis (type I) and Terranova (type II), the cestodes Otobothrium australe, Pterobothrium pearsoni, Pterobothrium sp. A, Callitetrarhynchus gracilis, Parotobothrium balli and Nybelinia sp., and the acanthocephalan Pomphorhynchus sp.] demonstrated little similarity between sites, indicating limited mixing and therefore long-term separation of post-juvenile fish. As such, the effects of fishing are likely to be localized within the current administrative boundaries, implying little need for interstate co-operative management. Within each jurisdiction, management of E. tetradactylum populations, including the establishment of harvest strategies and fishery regulations, should be conducted in a way that recognizes the resident nature of the fish.

Piloting education days for patients with early rheumatoid arthritis and their partners: a multidisciplinary approach.

OBJECTIVES: To develop, pilot, refine and reassess an education day presented by a rheumatology multidisciplinary team (MDT) for recently diagnosed (less than six months) rheumatoid arthritis (RA) patients and their partners/carers. METHODS: A patient education day was developed drawing on an assessment of local patient educational needs and preferences and input from a rheumatology MDT. Feedback from the first education day (2004) (Day 1; 12 patients; age range 19-63 years (median 46); 10 of whom were accompanied by a partner) informed the development of a second education day (2005) (Day 2; 19 patients; age range 36-75 years (median 57.5); 13 of whom were accompanied by a partner). Participants completed evaluation forms on both days and at follow-up between six and seven weeks later, rating each session on a 5-point scale on dimensions of 'informative', 'useful', 'interesting' and 'enjoyable'. A global rating of the day's 'usefulness' was completed at the end of each day on a 10-point scale. Participants were asked to write comments on each session and on aspects of the entire day. RA knowledge, and general and RA-specific self-efficacy were also measured on day 2 (and at follow-up) using the 12-item Patient Knowledge Questionnaire, the 10-item generalized self-efficacy scale and a four-item RA-specific self-efficacy scale. Both qualitative and quantitative methodologies were used in the analysis. RESULTS: Ratings for individual sessions were all high, with no session being rated below 4 out of 5 (1 = 'totally disagree' to 5 = 'totally agree') on both days. The majority of patients (84%) and their partners (57%) responded to the follow-up. Many had used the information package distributed on the day. Some patients and their partners reported positive changes in RA management. Although patient knowledge did not increase significantly (medians 11 at both time points, p = 0.054) (Day 2), RA self-efficacy improved (baseline 11 and 14, respectively), suggesting that patients were more confident in managing their condition (p = 0.010). CONCLUSIONS: The development of this 'local' education and information intervention was carried out in line with Medical Research Council guidelines, and the lessons learned from Day 1 informed further development for Day 2. A one-day format for education of early RA involving the rheumatology MDT was rated highly by participants and warrants further examination. Although this study was a small 'local' intervention, its strengths are that it informs the possibility of wider developments of this kind using a MDT.

A linkage map of the zebra finch Taeniopygia guttata provides new insights into avian genome evolution.

Passeriformes are the largest order of birds and one of the most widely studied groups in evolutionary biology and ecology. Until recently genomic tools in passerines relied on chicken genomic resources. Here we report the construction and analysis of a whole-genome linkage map for the zebra finch (Taeniopygia guttata) using a 354-bird pedigree. The map contains 876 SNPs dispersed across 45 linkage groups and we found only a few instances of interchromosomal rearrangement between the zebra finch and the chicken genomes. Interestingly, there was a greater than expected degree of intrachromosomal rearrangements compared to the chicken, suggesting that gene order is not conserved within avian chromosomes. At 1068 cM the map is approximately only one quarter the length of the chicken linkage map, providing further evidence that the chicken has an unusually high recombination rate. Male and female linkage-map lengths were similar, suggesting no heterochiasmy in the zebra finch. This whole-genome map is the first for any passerine and a valuable tool for the zebra finch genome sequence project and for studies of quantitative trait loci.

Information and self-management needs of people living with bleeding disorders: a survey.

The aim of the study was to conduct a survey of information and self-management needs among members of the UK Haemophilia Society (HS). Of 1082 members mailed, 307 took part in the survey, the mean age was 52.4 years (SD 14.8), 85% were male, 96% white and 66% were living with haemophilia A. 90% felt that there is a lack of understanding amongst the public regarding bleeding disorders, 76% felt that health professionals understood their health and information needs and 32% had experienced problems managing anxiety and or depression. Results suggest that there was a 'stigma' attached to having a bleeding disorder and almost half are very careful about informing others that they have a bleeding disorder. Self-efficacy was relatively high within the sample. Many respondents wanted to be kept informed about current research and the opportunity to participate in research. Women experienced a greater need for more information than men, particularly in the area of the 'medical management' and 'emotional' topics. Men appeared to need more information and or training on 'physical' aspects such as physiotherapy and joint replacement. The study provides an insight into the educational and self-management needs of those affected by bleeding disorders and highlights the importance of providing accessible information.

Investigation to find a suitable reference material for use as an inverse gas chromatography system suitability test.

The purpose of this study was to find a suitable material for use as a system suitability test material for inverse gas chromatography (IGC). The purpose of a system check is to measure the variability of the instrument being used rather than the material being analysed. Having such a system check for IGC enables a basic GMP requirement to be met. IGC results can then be used in regulatory submissions to support other already established techniques for characterising pharmaceutical materials. Alpha-alumina was chosen because it is inorganic, stable at high temperatures, resistant to hydration and is already established as a system check material for surface area determination by nitrogen adsorption. Two columns (1 and 2) packed from the same reference sample jar of alpha-alumina and analysed under the same conditions yielded dispersive surface energies of 34.8+/-0.8 and 35.3+/-0.8 mJ/m(2), respectively, at 15% RH. Column 1 was analysed on a second IGC system, and gave a dispersive surface energy of 34.7+/-0.2 mJ/m(2). No significant change was evident after 7 months storage under laboratory ambient conditions. Analysis of material from a second reference sample jar of alpha-alumina produced results not noticeably different to those of the first, yielding a dispersive surface energy of 35.3+/-0.1 mJ/m(2). A change was seen to occur in the surface properties alpha-alumina when the humidity was varied, but the change appeared to be consistent across the two columns reported. Based upon the data in this experimental, alpha-alumina appears to be a suitable material for use as a system suitability test material for IGC.

How well can common brushtail possums regulate their intake of Eucalyptus toxins?

We studied factors affecting the ability of common brushtail possums (Trichosurus vulpecula) to regulate their intake of a dietary toxin, jensenone, extracted from Eucalyptus leaves. Increasing concentrations of jensenone in the diet led to a dose-dependent decrease in food intake best described as an exponential decay. Animals that had not previously been exposed to jensenone ate significantly more when first offered food containing the compound than on subsequent days. However, when offered the same amount of food in a number of portions throughout the night, naive animals ate significantly less than animals offered the total meal at once. When offered food containing jensenone over a 13-day period, the animals' intake varied cyclically with relatively high food intakes followed by relatively low intakes. Furthermore, animals that were exposed to cold conditions (4 degrees C) ate more than those maintained at 18 degrees C but this difference was abolished when jensenone was included in the diet. We interpret these results as showing that regulation of toxin intake by common brushtail possums depends on learned responses that can override other important influences on feeding.