RESUMO
Investigation of activated partial thromboplastin time (APTT) in the plasma samples containing different levels of heparin determines the following parameters of the quality of APTT-control reagent kits: coefficient of variation, linearity, sensitivity, and detection. The kit is shown to meet GOST P 51352-99 requirements. The determination of the validity coefficient of approximation has proven a directly proportional functional relationship of the APTT values to the level of heparin in therapeutic ranges in the logarithmic coordinate system. The kit may be used to monitor heparin therapy.
Assuntos
Transtornos da Coagulação Sanguínea/sangue , Heparina/sangue , Tempo de Tromboplastina Parcial/normas , Kit de Reagentes para Diagnóstico/normas , Transtornos da Coagulação Sanguínea/tratamento farmacológico , Estudos de Viabilidade , Heparina/uso terapêutico , Humanos , Monitorização Fisiológica , Tempo de Tromboplastina Parcial/métodos , Garantia da Qualidade dos Cuidados de Saúde , Reprodutibilidade dos Testes , Sensibilidade e EspecificidadeRESUMO
An investigation into activated partial thromboplastin time (APTT) in the plasma samples with varying Factor VIII activities has determined the following parameters of the quality of APTT-control reagent kits: coefficient of variation, linearity, sensitivity, and detection. The kit is shown to meet GOST P 51352-99 requirements. The determination of the validity coefficient of approximation has proven a directly proportional functional relationship of the APTT values to the Factor VIII activity in the bilogarithmic coordinate system. The kit may be used to diagnose hemophilia A and Willebrand disease.
Assuntos
Fator VIII/metabolismo , Hemofilia A/diagnóstico , Tempo de Tromboplastina Parcial/normas , Plasma/metabolismo , Doenças de von Willebrand/diagnóstico , Fator VIII/genética , Hemofilia A/genética , Humanos , Tempo de Tromboplastina Parcial/métodos , Controle de Qualidade , Kit de Reagentes para Diagnóstico/estatística & dados numéricos , Padrões de Referência , Reprodutibilidade dos Testes , Sensibilidade e Especificidade , Doenças de von Willebrand/genéticaRESUMO
Fractionated phospholipids (PL) from soya beans were tried as partial thromboplastin (PT) in the activated partial thromboplastin time test. PL fractions were investigated for content of individual phospholipid classes, acidity and procoagulant activity as were PT from human brain and chromatographically pure PL. Procoagulant activity of phospholipid mixtures is higher than that of chromatographically pure PL and is unrelated to PL variety. Soya PL mixtures with human brain PT demonstrating high procoagulant activity are characterized by 0.3-0.8 ratio of total acid to total neutral PL and acid number 40-60 mg KOH. Isolated from soya PL procoagulants accelerate clotting of plasma from healthy subjects 2.2-fold being just a little less active than brain PL. This makes the above procoagulants usable as test-reagent for evaluation of hemostasis.
