[The efficiency of the enzyme immunoassay test system opisthorchiasis-CIC-EIA-best to detect circulating immune complexes containing opisthorchis antigens in the serum of patients with opisthorchiasis].

The efficacy of a kit of Opisthorchiasis-CIC-EIA-Best reagents was evaluated using 270 sera from patients in the study and control groups. The kit showed a sufficient sensitivity (not less than 87.2%) and a high specificity (not less than 97.9%). The use of the above kit of the reagents for enzyme immunoassay in practical healthcare enables one to increase detection rates among the infested subjects on comprehensive examination of those with suspected opisthorchiasis.

[Use of an enzyme immunoassay test system with cystic Echinococcus antigen to diagnose echinococcosis alveolaris (multilocularis) (alveococcosis)].

Twenty-three serum samples from 12 patients with Echinococcus alveolaris collected 1 to 12 years after surgery and 1--13 courses of specific therapy were used to evaluate the diagnostic effectiveness of an enzyme immunoassay (EIA) test system with cystic Echinococcus antigen. The findings enable the EIA test system with cystic Echinococcus antigen to be recommended for the diagnosis of Echinococcosis alveolaris and for the monitoring of its treatment results.

[Atopic dermatitis concurrent with toxoplasmosis in the Republic of Dagestan: epidemiological aspects].

[By using a group of patients with atopic dermatitis who live in Dagestan as an example, the authors show the high prevalence of toxoplasmosis among them. This requires new approaches to diagnosing and treating atopic dermatitis to be introduced in this region]

[Optimization of serological diagnosis of cystic echinococcosus (granulosus) infection].

The diagnostic effectiveness of instrumental analysis of the concentration of specific antibodies, which was expressed in antibody units and titers, was compared in the test system of enzyme immunoassay using Echinococcus granulosus on 76 serum samples from patients with echinococcosis. The factors of possible influence on the instrumental readings of enzyme immunoassay are presented. A correlation was found in the specific antibody concentrations expressed in antibody units and titers.

[Consideration of the results of enzyme immunoassay in tissue helminthisms in antibody units].

Two hundred and twenty-two serum samples from patients infected with Toxocara, 374 from those infected with Echinococcus alveolaris, and 200 from healthy blood donors, which were screened in the enzyme immunoassay system, were used to assess an instrumental method for considering the assay results expressed in antibody units (AU), A direct correlation was found between the values of the intensity of an immune response in the infected, which were expressed in AU and titers routinely used for this purpose. Introducing the optic density index, standardized by the manufacturer of a reference test-positive sample kit, into the formula to calculate AU reduces possible variations in the assay results both between different batches and within one batch of kits.

[The first case of diagnosis of dirofilariasis from the microfilariae detected in the human subcutaneous tumor punctate]. / Pervyi sluchai diagnostiki dirofiliarioza po mikrofiliariiam, obnaruzhennym v punktate podkozhnoi opukholi cheloveka.

The paper describes the first case of diagnosis of dirofilariasis from the microfilariae detected in the subcutaneous tumor punctate from a person who has the clinical manifestations of D. repens invasion and resides in the Saratov Region that is endemic in dirofilariasis. The fact of detection of microfilariae in the man undergone invasion is assessed not only as the first case in the lifetime diagnosis of the first case of diagnosis of subcutaneous dirofilariasis, but also as the first case of verification of that a female can achieve sexual maturity in the organism of an accidental host and its impregnation if there are even single specimens of both sexes, which is characteristic in human invasiveness. On this basis, the authors consider it wrong to refer this helminthiasis to as a group of diseases defined as "Larva migrans cutaneous" since this is inconsistent with the biology of a causative agent that accomplishes migration at the adult stage in the host's organism and does not orient a physician to establish a correct clinical and parasitological diagnosis.

[Human dirofilariasis in the Moscow region]. / Difiliarioz cheloveka v Moskovskom regione.

Dirofilaria repens Railliet et Henry, 1911 was for the first time studied in the Moscow Region where 11 patients (6 males and 5 females) aged 4-72 years were recorded in 2000-2002. The geographical distribution (the southern and south-eastern parts of the region) of local cases of dirofiliriasis, was observed. There was a relatively high rate of D. repens in the male genitalia of the infected patients as compared with that reported by other authors. In accordance with the current terminology, D. repens in the Moscow Region is proposed to be regarded as emerging infection. The authors show it necessary to obligatorily record all autochtonous cases of D. repens infection, to make epidemiological surveillance and analysis in order to early detect and prevent dirofilariasis-associated complications.

[Clinical and parasitological diagnosis of human dirofilariasis]. / Klinicheskii i parazitologicheskii diagnoz dirofiliarioza cheloveka.

The paper reports two new cases of human dirofiliriasis (its causative agent is Dirofiliria repens) with a rare clinical diagnosis of funiculitis of the right scrotal spermatic cord in a 12-year-old boy from the Tambov Region and of erysipelatous inflammation of the outer side of the right leg in a 68-year-old woman who resides in Moscow and lives in her country house in the Shakhovsky District, Moscow Region, in summer. Both cases of invasion are regarded as local. The bodies of the D. repens females detected measured 110 x 0.476-0.48 and 135 x 0.56-0.60 mm, respectively, and the females were identified as impubertal and pubertal, but infecund. In the former case, dirofiliriasis was an etiological factor of spermatic cord funiculitis. In the latter case, the location of Dirofiliria provoked prolonged (7-month) erysipelatous inflammation. Emphasis is placed on the implication of dirofiliriasis in human pathology. To have a more intimate knowledge of parasitic etiology will help physicians in making a differential diagnosis of dirofiliriasis and in elaborating a correct treatment policy after a clinical and parasitological diagnosis is established.

[A dynamic study of the immune response of the population in a focus of hydatid disease in Dzhambul Province, Kazakhstan]. / Izuchenie dinamiki immunnogo otveta naseleniia v ochage gidatidoznogo ékhinokokkoza v Dzhambul'skoi oblasti Kazakhstana.

Screening for hydatid disease in foci should be carried out making use of the two most effective immunologic methods, indirect hemagglutination (IHA) and enzyme immunoassay (EIA). In order to have more reliable data on the structure of infection focus and for an earlier detection of patients such studies should be carried out over time and be followed by clinical examinations of subjects in whom anti-Echinococcus antibodies are repeatedly detected in epidemiologically significant and higher titers in IHA and/or EIA. All seropositive subjects and patients operated on for hydatid disease should be followed up.