Parotidite aguda em jovem adulto sistemicamente saudável. Relato de caso / Acute parotitis in a systemically healthy young adult. A case report

Objetivo: Este estudo relata um caso incomum de parotidite aguda em um paciente adulto jovem, saudável e com boa higiene oral. Relato de Caso: O paciente procurou o Departamento de Estomatologia, na Universidade Estadual de Ponta Grossa devido a um inchaço na região da glândula parótida direita. O paciente foi submetido a exames clínicos e radiológicos e foi prescrito tratamentos com drogas anti-inflamatórias e antibióticos. No quinto dia os sinais e sintomas haviam desaparecido. Conclusão: O desenvolvimento de parotidite aguda em adultos jovens saudáveis com uma boa higiene oral é possível, mas isso torna o diagnóstico mais difícil. Anti-inflamatórios, antibióticos e fisioterapia associada com calor pode contribuir para a recuperação dos sinais e sintomas após 5 dias de tratamento.

Aim: This study reports an unusual case of acute parotitis in a healthy young adult with good oral hygiene. Case Report: The patient sought the Department of Stomatology, at Ponta Grossa State University due to a swollen on the right parotid gland region. The patient underwent clinical and radiological treatments and was prescribed anti-inflammatory drugs and antibiotics. On the fifth day the signs and symptoms had disappeared. Conclusion: The development of acute parotitis in healthy young adults with good oral hygiene is possible, but this makes the diagnosis more difficult. Anti-inflammatory, antibiotic and heat physiotherapy can contribute to the recovery of the signs and symptoms after 5 days of treatment.

Platelet-rich plasma stimulates cytokine expression and alkaline phosphatase activity in osteoblast-derived osteosarcoma cells.

OBJECTIVE: The aim of this study was to investigate the effects of PRP on SAOS-2 cells in terms of cytokine expression, cell activity and oxidative stress. DESIGN: Cell line SAOS-2 (1×10(5)cells/mL) were grown in culture medium α-MEM with 10% FBS for 24h and stimulated (or not) with PRP at concentrations of 3, 10 and 20%, LPS (E. coli, 10g/mL) and IL-1ß (1mg/mL) for 24h. The supernatant was collected and analyzed for the expression of cytokines in a panel array, ALP using a commercial kit and NO(2)(-) with Griess reaction method. Also, the cells were analyzed using Western blot for RANKL and slot blotting for nitrotyrosine expression. RESULT: There were no significant differences amongst the groups in terms of NO(2)(-), protein nitrotyrosine content and RANKL expression. However, all stimuli increased ALP activity and in case of PRP, it was in a dose-dependent manner (p<0.001). Also, all stimuli induced an increase in cytokines and chemokines expression, but only PRP promoted an increase of component C5, sICAM-1 and RANTES expression. Whilst IL-1 receptor antagonist (IL-1ra) expression was down-regulated by PRP, both LPS and IL-1ß caused up-regulation of this cytokine. CONCLUSIONS: PRP can stimulate osteoblast activity and cytokine/chemokine release, as well as indicate some of the mediators that can (and cannot) be involved in this activation.

The effect of supra- and subphysiologic testosterone levels on ligature-induced bone loss in rats--a radiographic and histologic pilot study.

BACKGROUND: Testosterone is the primary male sexual hormone, and varying concentrations of the hormone mediated by physiologic, pathologic, or pharmacologic mechanisms may induce large variations in the body. Data regarding the general role of testosterone in mediating inflammation are still inconclusive. Therefore, the purpose of this study is to assess the consequences of supra- and subphysiologic levels of testosterone on ligature-induced bone loss in rats. METHODS: Three male adult Holtzman rats were used to observe the course of serum testosterone concentration following orchiectomy (Ocx) and testosterone injections. Another 60 rats were randomly assigned to the following groups: (1) sham-operation controls (n = 10); (2) sham-operation and ligature-induced bone loss (n = 10); (3) orchiectomy without ligature (Ocx; n = 10); (4) Ocx and ligature (n = 10); (5) Ocx plus 250 mg/kg body weight intramuscular testosterone esters injection without ligature (Ocx+T; n = 10); and (6) Ocx, T, and ligature (n = 10). The ligatures were placed 30 days postorchiectomy (or sham-operation) and maintained for 15 days. Thereafter, the rats were sacrificed, and their hemimandibles were used for radiographic evaluation of bone loss along with histologic and histometric analyses of gingival tissue. RESULTS: The results indicated a significant increase in bone loss in the Ocx and Ocx+T groups in the presence and absence of inflammation, respectively. In addition, the Ocx and Ocx+T groups presented increased gingival area accompanying ligature-induced bone loss. CONCLUSIONS: Both sub- and supraphysiologic testosterone levels may influence bone metabolism, but only subphysiologic levels significantly increase ligature-induced bone loss. Moreover, testosterone has a regulatory effect on the gingival area.