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1.
Food Chem ; 453: 139690, 2024 Sep 30.
Artigo em Inglês | MEDLINE | ID: mdl-38781903

RESUMO

Jabuticaba peel, rich in antioxidants, offering health benefits. In this study, the extraction of phenolic compounds from jabuticaba peel using ultrasound-assisted (UA) and their subsequent concentration by nanofiltration (NF) employing a polyamide 200 Da membrane was evaluated. The UA extractions were conducted using the Central Composite Rotatable Design (CCRD) 22 methodology, with independent variables extraction time (11.55 to 138 min) and temperature (16.87 to 53.3 °C), and fixed variables mass to ethanol solution concentration at pH 1.0 (1:25 g/mL), granulometry (1 mm), and ultrasonic power (52.8 W). The maximum concentrations obtained were 700.94 mg CE/100 g for anthocyanins, 945.21 mg QE/100 g for flavonoids, 133.19 mg GAE/g for phenols, and an antioxidant activity IC50 of 24.36 µg/mL. Key phenolic compounds identified included cyanidin-3-glucoside, delphinidin-3-glucoside, and various acids like syringic and gallic. NF successfully concentrated these compounds, enhancing their yield by up to 45%. UA and NF integrate for sustainable extraction.


Assuntos
Antioxidantes , Frutas , Fenóis , Extratos Vegetais , Fenóis/química , Fenóis/isolamento & purificação , Extratos Vegetais/química , Extratos Vegetais/isolamento & purificação , Frutas/química , Antioxidantes/química , Antioxidantes/isolamento & purificação , Filtração , Myrtaceae/química , Antocianinas/química , Antocianinas/isolamento & purificação , Fracionamento Químico/métodos
2.
Environ Res ; 250: 118501, 2024 Jun 01.
Artigo em Inglês | MEDLINE | ID: mdl-38367841

RESUMO

This study explores the application of a tyrosinase cantilever nanobiosensor for detecting 17ß-estradiol and estrone in typical water systems. The physical-chemical parameters of water were evaluated within the Tigre River micro-basin in Erechim, RS, to determine water potability for urban populations. Water clarity, conductivity, and pH levels were essential markers, adhering to recognized standards for water quality and human consumption. The cantilever nanobiosensor demonstrated strong sensitivity and a broad linear range, with a limit of detection (<0.00051 ppb) surpassing other enzymatic biosensors and covering a range of 0.0001-100 ppb. The real water sample quality investigated in relation to contamination with 17ß-estradiol and estrone by nanobiosensor showed values below the LOD for both compounds. Recovery studies demonstrated the reliability of the nanobiosensor. Selectivity tests indicated minimal interference from structurally similar substances. This study validates the nanobiosensor's potential for environmental monitoring and hormone detection, aligning with standard practices.


Assuntos
Técnicas Biossensoriais , Monitoramento Ambiental , Monofenol Mono-Oxigenase , Rios , Poluentes Químicos da Água , Monitoramento Ambiental/métodos , Monitoramento Ambiental/instrumentação , Rios/química , Técnicas Biossensoriais/instrumentação , Técnicas Biossensoriais/métodos , Poluentes Químicos da Água/análise , Estradiol/análise , Estrona/análise , Limite de Detecção
3.
Food Technol Biotechnol ; 61(3): 312-327, 2023 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-38022879

RESUMO

Cynara cardunculus L. var. scolymus, known as the artichoke, originated in the Mediterranean region and is now cultivated in several countries. The artichoke has leaves, a stem, and a head, also called a floral capitulum, covered with green and pointed bracts. It is rich in polyphenols, flavonoids, anthocyanins, phenolic compounds, inulin, coumarins, terpenes, dietary fibre, enzymes, polysaccharides, minerals and vitamins, and therefore has a wide range of uses, including in the food industry, medicine and biofuels. Several studies have shown that artichokes have properties such as antioxidant, anti-inflammatory, antimicrobial, anticancer, hypocholesterolaemic, anti-HIV, cardioprotective, hepatoprotective and lipid-lowering effects. The aim of this study is to provide a literature review on the phytochemical composition, bioactivity and applications, focusing on the methods of extraction, purification and concentration of enzymes present in artichoke.

4.
Micromachines (Basel) ; 14(10)2023 Sep 23.
Artigo em Inglês | MEDLINE | ID: mdl-37893256

RESUMO

This study presents a comprehensive literature review that investigates the distinctions between true and false cinnamon. Given the intricate compositions of essential oils (EOs), various discrimination approaches were explored to ensure quality, safety, and authenticity, thereby establishing consumer confidence. Through the utilization of physical-chemical and instrumental analyses, the purity of EOs was evaluated via qualitative and quantitative assessments, enabling the identification of constituents or compounds within the oils. Consequently, a diverse array of techniques has been documented, encompassing organoleptic, physical, chemical, and instrumental methodologies, such as spectroscopic and chromatographic methods. Electronic noses (e-noses) exhibit significant potential for identifying cinnamon adulteration, presenting a rapid, non-destructive, and cost-effective approach. Leveraging their capability to detect and analyze volatile organic compound (VOC) profiles, e-noses can contribute to ensuring authenticity and quality in the food and fragrance industries. Continued research and development efforts in this domain will assuredly augment the capacities of this promising avenue, which is the utilization of Artificial Intelligence (AI) and Machine Learning (ML) algorithms in conjunction with spectroscopic data to combat cinnamon adulteration.

5.
Artigo em Inglês | MEDLINE | ID: mdl-37567647

RESUMO

In a cross-sectional study of gymnasium users (both sexes, ages = 41.9 ± 14.8 years), we examined the moderating role of macronutrient intake in relation to body composition and genotoxicity. A questionnaire was administered to evaluate characteristics of the participants. To assess macronutrient consumption, we used 24-h food recalls on three non-consecutive days. Body composition (body fat percentage and muscle mass) was evaluated with a bioimpedance scale. Genotoxicity was assessed with the buccal micronucleus cytome assay. Multiple linear regression models were applied, adjusting for age; sex; tobacco and alcohol consumption; and (with regard to exercise habits) frequency, training time, intensity, and types. Micronucleus frequency was directly associated with body fat and inversely associated with muscle mass. Our study shows that carbohydrate and fat intakes affect body fat percentage and micronucleus frequency in gymnasium users.


Assuntos
Academias de Ginástica , Masculino , Feminino , Humanos , Estudos Transversais , Índice de Massa Corporal , Ingestão de Alimentos , Composição Corporal , Exercício Físico/fisiologia
6.
World J Microbiol Biotechnol ; 39(8): 201, 2023 May 19.
Artigo em Inglês | MEDLINE | ID: mdl-37202540

RESUMO

The objective was to isolate lactic acid bacteria (LAB) from southern Brazil's wines and investigate their potential as starter cultures for malolactic fermentation (MLF) in Merlot (ME) and Cabernet Sauvignon (CS) wines through the fermentative capacity. The LAB were isolated from CS, ME, and Pinot Noir (PN) wines in the 2016 and 2017 harvests and evaluated for morphological (color and shape of the colonies), genetic, fermentative (increase in pH, acidity reduction, preservation of anthocyanins, decarboxylation of L-malic acid, yield of L-lactic acid, and content of reduced sugars), and sensory characteristics. Four strains were identified as Oenococcus oeni [CS(16)3B1, ME(16)1A1, ME(17)26, and PN(17)65], one as Lactiplantibacillus plantarum [PN(17)75], and one as Paucilactobacillus suebicus [CS(17)5]. Isolates were evaluated in the MLF and compared to a commercial strain (O. oeni), as well as a control (without inoculation and spontaneous MLF), and standard (without MLF). CS(16)3B1 and ME(17)26 isolates finished the MLF for CS and ME wines, respectively, after 35 days, similar to the commercial strain, and CS(17)5 and ME(16)1A1 isolates ended the MLF in 45 days. In the sensory analysis, ME wines with isolated strains received better scores for flavor and overall quality than the control. Compared to the commercial strain, CS(16)3B1 isolate obtained the highest scores for buttery flavor and taste persistence. CS(17)5 isolate received the higher scores for a fruity flavor and overall quality and the lowest for a buttery flavor. The native LAB displayed MLF potential, regardless of the year and grape species from which they were isolated.


Assuntos
Lactobacillales , Oenococcus , Vinho , Vinho/microbiologia , Brasil , Lactobacillales/genética , Fermentação , Antocianinas , Oenococcus/genética , Malatos
7.
Mol Cell Biochem ; 478(8): 1719-1725, 2023 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-36564575

RESUMO

High sugar intake is a major risk factor for metabolic disorders. Genotoxicity is an important factor in diabetes onset, and iron (Fe) may be an aggravating element. However, this relationship is still poorly established. Thus, this study evaluated whether Fe supplementation could aggravate obesity, impaired glucose tolerance, and sugar overload-induced genotoxicity in rats. A total of 24 rats were treated with different diets: standard diet (SD, n = 8), invert sugar overload (320 g/L, HSD, n = 8), or Fe plus invert sugar overload (2.56 mg/L of Fe2+, Fe-HSD, n = 8) for four months. After treatment, the Fe-HSD group showed no excessive weight gain or impaired glucose tolerance. DNA damage in blood, as assessed by comet assay, gradually increased in HSD during treatment (p < 0.001), whereas Fe-HSD showed a nonlinear increase in DNA damage. Moreover, Fe-HSD presented 0.6-fold more DNA damage compared with SD (p = 0.0055) in the 1st month of treatment. At months 2 and 3, results show a ≥ 1.4-fold increase in HSD and Fe-HSD DNA damage, respectively, compared with SD (p < 0.01). At the end of the experiment, only HSD DNA damage differed from SD (1.5-fold more, p = 0.0196). Fe supplementation did not aggravate the invert sugar-induced DNA damage (p > 0.05). In the pancreas, results showed no differences in DNA damage. Mutagenicity, evaluated by micronucleus testing, was not observed regardless of treatment (p = 0.428). Fe supplementation, in the evaluated concentration, did not aggravate weight gain, impaired glucose tolerance, and sugar overload-induced genotoxicity in rats.


Assuntos
Intolerância à Glucose , Ferro , Ratos , Animais , Açúcares , Dano ao DNA , Aumento de Peso , Suplementos Nutricionais
8.
Food Sci Technol Int ; 29(4): 310-317, 2023 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-35238680

RESUMO

This study evaluated the influence of O2-absorbing sachets into different packages polyethylene terephthalate (PET - E1), low density polyethylene (LDPE - E2), and PET/LDPE (E3) on the quality of minimally processed soybean sprouts (MP-sprouts). The MP-sprouts were stored up to 12 days and characterized for physicochemical, microbiological and sensory attributes. The O2-absorbing sachet showed changes during exposure to the environment by the formation of amorphous iron hydroxide, which was observed by the analysis of functional groups and XRD. The packaging used in the study showed high transmittance and clarity, and low Haze, being a good feature for storing the MP product. All packaging systems showed an increase in the volume of absorbed oxygen (cm3) up to 9 days of storage. The physic-chemical characteristics of the MP-sprouts stored for 12 days were preserved in the different packages, showing no difference regarding the use of the O2-absorbing sachet. The O2-absorbing sachet not influenced the quality of MP-sprouts during the stored (12 days). The best visual aspect was observed in PET package (with and without O2-absorbing sachet) being classified as excellent, without color change. The MP-sprouts also fulfilled the microbiological quality standards and presented 75.11% acceptability and 72.40% purchase intention.


Assuntos
Embalagem de Alimentos , Conservação de Alimentos , Embalagem de Alimentos/métodos , Conservação de Alimentos/métodos , Glycine max , Polietileno , Oxigênio
9.
Meat Sci ; 194: 108966, 2022 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-36126391

RESUMO

The objective of this work was to develop, characterize and evaluate the application of active edible films based on gelatin and green tea extract in coating of fresh sausages. The green tea extract showed IC50 of 0.088 mg/mL and minimum inhibitory concentrations of 0.05 mg/mL for Listeria monocytogenes, 0.025 mg/mL for Staphylococcus aureus, 0.04 mg/mL for Escherichia coli, and >1.0 mg/mL for Salmonella enterica serovar Choleraesuis. The formulation with 15% (w/v) of gelatin and 30% (w/w) of glycerol showed better adhesion and appearance in the coating of the product. When using 1.0% of green tea extract, the lowest IC50, was obtained and the antioxidant activity was maintained for 35 days. There was a more accentuated decrease in pH and an increase in acidity and peroxide index in fresh sausages without film compared to those coated with the active film (1.0% of green tea extract) during storage. In addition, it was found that the use of active gelatin film (1.0% of green tea extract) kept the TBARS indexes of fresh sausage samples lower than the standard (without coating) and of films containing only gelatin, after 48 days of storage.


Assuntos
Antioxidantes , Filmes Comestíveis , Antioxidantes/farmacologia , Gelatina/química , Extratos Vegetais/farmacologia , Escherichia coli , Chá/química
10.
Anal Methods ; 13(38): 4528, 2021 Oct 08.
Artigo em Inglês | MEDLINE | ID: mdl-34581319

RESUMO

Correction for 'Sexual pheromone detection using PANI·Ag nanohybrid and PANI/PSS nanocomposite nanosensors' by Janine Martinazzo et al., Anal. Methods, 2021, 13, 3900-3908, DOI: 10.1039/d1ay00987g.

11.
Anal Methods ; 13(35): 3900-3908, 2021 09 16.
Artigo em Inglês | MEDLINE | ID: mdl-34558574

RESUMO

In this study, polyaniline/poly(styrene sulfonate) (PANI/PSS) nanocomposite and polyaniline·silver (PANI·Ag) nanohybrid thin films were obtained in cantilever nanosensors surface. The developed films were characterized in relation to topography, roughness, thickness, height, and structural properties. The topography study revealed that both films have a globular morphology, thickness and height in nanoscale. The gas sensing performance was investigated for sexual pheromone from the neotropical brown stink bug, Euschistus heros (F.). The sensitivities of both nanosensors based on PANI/PSS nanocomposite and PANI·Ag nanohybrid films were similar. The PANI·Ag nanohybrid nanosensor had a limit of detection of less than 3.1 ppq and limit of quantification of 10.05 ppq. The nanosensor layers were analyzed by UV-vis and FTIR showing the incorporation of Ag nanoparticles in the nanohybrid. We found that pheromone compound was adsorbed in sensing layer resulting in a reduction in the resonance frequency. The detection mechanism help us understand the good results of LOD, LOQ, sensitivity, selectivity and repeatability. The presented device has great potential for detection of the sexual pheromone from E. heros.


Assuntos
Nanopartículas Metálicas , Nanocompostos , Atrativos Sexuais , Feromônios , Prata
12.
ACS Sens ; 5(6): 1814-1821, 2020 06 26.
Artigo em Inglês | MEDLINE | ID: mdl-32515185

RESUMO

This work describes the development of an electronic nose (e-nose) based on carbon nanocomposites to detect clove essential oil (CEO), eugenol (EUG), and eugenyl acetate (EUG.ACET). Our e-nose system comprises an array of six sensing units modified with nanocomposites of poly(aniline), graphene oxide, and multiwalled carbon nanotubes doped with different acids, dodecyl benzene sulfonic acid, camphorsulfonic acid, and hydrochloric acid. The e-nose presented an excellent analytical performance to the detected analytes (CEO, EUG, and EUG.ACET) with high sensitivity and reversibility. The limit of detection was lower than 1.045 ppb, with response time (<13.26 s) and recovery time (<106.29 s) and low hysteresis. Information visualization methods (PCA and IDMAP) demonstrated that the e-nose was efficient to discriminate the different concentrations of analyte volatile oil compounds. PM-IRRAS measurements suggest that the doping mechanism of molecular architectures is composed of a change in the oscillation energy of the characteristic dipoles and changes in the molecular orientation dipoles C═C and C═O at 1615 and 1740 cm-1, respectively. The experimental results indicate that our e-nose system is promising for a rapid analysis method to monitor the quality of essential oils.


Assuntos
Nanocompostos , Nanotubos de Carbono , Óleos Voláteis , Syzygium , Óleo de Cravo , Nariz Eletrônico
13.
Food Sci Technol Int ; 26(8): 657-665, 2020 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-32326775

RESUMO

The aim of this study was to concentrate whey protein by ultrafiltration process, evaluating the pressure at 1-3 bar and temperature of 10-20℃. In the conditions that show the more protein concentration were evaluated the interfacial and emulsifying properties at pH 5.7 and 7.0. The whey concentrate at 10℃ and 1.5 bar showed the higher protein value 36% (w/w), with soluble protein of 33.82% (solubility of 93.94%) for pH 5.7 and 34% (solubility of 94.4%) for pH 7.0, respectively. The whey concentrate powder present particle size distribution between 0.4-110 um. The whey at pH 5.7 and 7.0 was not observed significant differences in the resistance parameters of the oil/water layer interface. The interfacial film formed by the proteins presented an essentially elastic behavior in both pH, and in pH 5.7 the emulsion was more stable with lower diameter droplets. The concentrate whey showed techno-functional properties (emulsification and solubility), which allow the use as ingredients in products of industrial interest in food products such as mayonnaise, ice cream, sauces, and others.


Assuntos
Ultrafiltração , Proteínas do Soro do Leite , Emulsões/química , Concentração de Íons de Hidrogênio , Solubilidade , Proteínas do Soro do Leite/química
14.
J Environ Sci Health B ; 55(3): 239-249, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-31680618

RESUMO

Heavy metals can be highly toxic depending on the dose and the chemical form. In this context, sensing devices such as nanobiosensors have been presented as a promising tool to monitor contaminants at micro and nanoscale. In this work, cantilever nanobiosensors with phosphatase alkaline were developed and applied to detect heavy metals (Pb, Ni, Cd, Zn, Co, and Al) in river water. The nanobiosensor surface was functionalized by the self-assembled monolayers (SAM) technique using 16-mercaptohexadecanoic acid, N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide (EDC) and N- hydroxysuccinimide (NHS), and phosphatase alkaline enzyme. The sensing layer deposited on the cantilever surface presented a uniform morphology, at nanoscale, with 80 nm of thickness. The nanobiosensor showed a detection limit in the ppb range and high sensitivity, with a stability of fifteen days. The developed cantilever nanobiosensor is a simple tool, suitable for the direct detection of contaminants in river water.


Assuntos
Técnicas Biossensoriais/instrumentação , Metais Pesados/análise , Rios/química , Poluentes Químicos da Água/análise , Fosfatase Alcalina/química , Fosfatase Alcalina/metabolismo , Técnicas Biossensoriais/métodos , Brasil , Carbodi-Imidas/química , Monitoramento Ambiental/instrumentação , Monitoramento Ambiental/métodos , Desenho de Equipamento , Limite de Detecção , Metilaminas/química , Ácidos Palmíticos/química , Sensibilidade e Especificidade
15.
Appl Biochem Biotechnol ; 190(4): 1512-1524, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-31784877

RESUMO

This work aimed to develop cantilever nanobiosensor functionalized with tyrosinase enzyme to detect 17ß-estradiol and estrone hormones. In this system, the tyrosinase enzyme was covalently immobilized by self-assembled monolayer onto the cantilever sensor surface. It was possible to verify that the high hormone concentration investigated resulted in high voltage response. The nanobiosensor presented a distinction between the concentrations evaluated and was verified sensitivities of 0.497 and 0.101 V/µg, limit of detection of 0.1 and 0.4 ng/L for the hormones 17ß-estradiol and estrone, respectively. The device showed good reversibility and during 30 days of storage maintained about 99% of the original signal. The cantilever nanobiosensor applied in different water samples (ultrapure, river, tap, and mineral) showed good performance, so could be readily extended toward the on-site monitoring of the other trace small molecular pollutants in environmental water matrices.


Assuntos
Técnicas Biossensoriais , Monitoramento Ambiental/instrumentação , Monitoramento Ambiental/métodos , Estradiol/análise , Estrona/análise , Monofenol Mono-Oxigenase/química , Nanopartículas/química , Poluentes Químicos da Água/análise , Poluentes Ambientais/análise , Limite de Detecção , Microscopia de Força Atômica , Microscopia Eletrônica de Varredura , Nanotecnologia , Rios , Silício/química , Esteroides , Propriedades de Superfície , Água/química
16.
Food Res Int ; 113: 309-315, 2018 11.
Artigo em Inglês | MEDLINE | ID: mdl-30195525

RESUMO

Aroma is closely related to the food product acceptability and an important product quality indicator. Electronic-nose (E-nose) systems are an interesting alternative to traditional methods of aroma analyses. A lab-made E-nose system equipped with an array of sensing units comprised by gold interdigitated microelectrodes (IDEs) using polyaniline (Pani) as sensitive layers deposited by the in situ and Layer-by-layer (LbL) methods was used to analyze aromas in gummy candies. Different concentrations from artificial aromas (apple, strawberry and grape), added to the gummy candies were evaluated. Our system presented 21.6 mV.ppb-1 sensitivity, ppb range detection limit, and good reversibility, around 97.6%. The sensitive layers of Pani films was adequate deposited on IDEs observed by the Attenuated Total Reflection/Fourier-transform infrared spectroscopy (ATR/FTIR). Linear Discriminant Analysis (LDA) was able to classify apple, strawberry, and grape aromas added to gummy candies using saturation potential values from the E-nose system, demonstrating its applicability in food matrices.


Assuntos
Compostos de Anilina/química , Doces/análise , Nariz Eletrônico , Análise de Alimentos/instrumentação , Odorantes/análise , Análise Discriminante , Desenho de Equipamento , Análise de Alimentos/métodos , Ouro , Limite de Detecção , Microeletrodos , Espectroscopia de Infravermelho com Transformada de Fourier
17.
Poult Sci ; 97(12): 4462-4469, 2018 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-30101324

RESUMO

This study aimed to evaluate the parameters that influence the water absorption and drip of chicken carcasses due to the processing and pre-cooling of the meat in an industrial chiller. A total of 1,179 chickens were sampled during industrial processing to evaluate the influence of variables, validate the parameters, and conduct histological analysis. The best parameters for guaranteeing absorption levels and drip tests within acceptable limits on chicken carcasses were total residence time of 60 min (in the pre-chiller, chiller 1, and chiller 2); air pressure of chillers at 0.5 bar; the abdominal opening of carcasses at a maximum of 2 cm. These parameters did not influence the protein content, moisture/protein ratio, pH, or lipid content. The validation of the parameters and the histological analysis performed after each cooling stage showed that the most significant structural changes occurred in the pre-chiller, where the temperature of carcasses and water was higher, which contributes to greater absorption.


Assuntos
Temperatura Baixa , Manipulação de Alimentos , Carne/análise , Músculos Peitorais/fisiologia , Água/análise , Adsorção , Animais , Galinhas
18.
Scanning ; 2018: 4782685, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-29967658

RESUMO

This paper describes the use of different conducting polymers (polyaniline, poly(o-ethoxyaniline), and polypyrrole) as a sensitive layer on a silicon cantilever sensor. The mechanical response (deflection) of the bimaterial (the coated cantilever) was investigated under the influence of relative humidity. The variations in the deflection of the coated cantilevers when exposed to relative humidity were evaluated. The results indicated a linear sensitivity in ranges, where the high value was obtained for a polypyrrole-sensitive layer between 20 and 45% of humidity. Furthermore, the sensor shows excellent performance along with rapid response and recovery times, relatively low hysteresis, and excellent stability. The sensors developed are potentially excellent materials for sensing low humidity for long time.

19.
Anal Bioanal Chem ; 410(17): 4091-4108, 2018 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-29926152

RESUMO

Semiochemicals are chemical compounds that are released by many species as a means of intra- and interspecific communication. Insects have extremely advanced olfactory systems; indeed, they rely on smell when performing many of their main behaviors, such as oviposition, breeding, prey location, and defense. This characteristic of insects implies that semiochemicals could be used for various applications, including in agriculture, where they could be employed along with other tools to control pest insects. The aim of this review is to present the main techniques used and the state of the art in the detection of semiochemicals, focusing on pheromones. In addition to the traditional methods of identifying semiochemicals, such as gas chromatography coupled to a high-resolution detection mode (e.g., flame ionization (FID), electron capture (ECD), photoionization (PID), or mass spectrometry (MS)), other tools are addressed in this review, including sensors and biosensors. While these new technologies may be used under laboratory conditions to improve or complement technologies that are already being used, they are mainly intended for use as new agricultural tools for detecting and controlling pest insects in the field.


Assuntos
Técnicas Biossensoriais , Insetos , Feromônios/química , Agricultura , Animais , Técnicas Biossensoriais/métodos
20.
Appl Biochem Biotechnol ; 186(4): 1061-1073, 2018 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-29862444

RESUMO

A cantilever nanobiosensor functionalized with vegetable source of peroxidase was developed as an innovative way for glyphosate herbicide detection over a wide concentration range (0.01 to 10 mg L-1) using atomic force microscopy (AFM) technique. The extract obtained from zucchini (Cucurbita pepo source of peroxidase), with high enzymatic activity and stability has been used as bio-recognition element to develop a nanobiosensor. The polarization-modulated reflection absorption infrared spectroscopy (PM-RAIRS) demonstrated the deposition of enzyme on cantilever surface using self-assembled monolayers (SAM) by the presence of the amide I and II bands. The detection mechanism of glyphosate was based on the changes in surface tension caused by the analyte adsorption, resulting in a conformational change in the enzyme structure. In this way, the results of nanobiosensor demonstrate the potential of the sensing device for detecting glyphosate with a detection limit of 0.028 mg L-1.


Assuntos
Técnicas Biossensoriais/métodos , Cucurbita/enzimologia , Glicina/análogos & derivados , Peroxidase/química , Proteínas de Plantas/química , Glicina/análise , Glifosato
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