Morphological examination of the visual system and orbital region in the red panda (Ailurus fulgens fulgens).

OBJECTIVES: The red panda is currently the only surviving member of the Ailuridae family in the Caniformia suborder. In this study, we provide data on anatomical, morphometric, histological and histochemical examination of the orbital region, eyelids, orbital gland, and eye tunics in two adult males Ailurus fulgens fulgens from the Wroclaw Zoological Garden, Poland. METHODS: The study involved morphometric analysis of the eyeball and selected accessory organs of the eye, along with analysis of the bony orbit, including its morphometry, macroscopic, and microscopic evaluation. Microscopic evaluation encompassed histological and histochemical staining, with the former involving hematoxylin & eosin (H&E), Movat pentachrome, picro-Mallory trichrome, Fontana-Masson, and the latter including PAS, AB pH 1.0, AB pH 2.5; AB pH 2.5/PAS, and HDI. RESULTS: The upper (UE) and lower (LE) eyelids presented well-developed tarsal glands, sebaceous glands, and a characteristic simple alveolar gland (producing a mucous secretion). The palpebral part of the lacrimal gland was present. A single lymphoid follicle was observed only in the upper eyelids. The superficial gland of the third eyelid (SGTE) was a multilobar acinar complex that produces mucous secretion and is contained within the interlobular ducts of numerous aggregates of lymph nodes. The third eyelid (TE) was T-shaped and composed of hyaline tissue, containing CALT. The lacrimal gland (LG) also revealed a multilobar acinar complex that produced mucous secretion, with a single lymphoid follicle. The cornea consisted of 4 layers, as Bowman's membrane was absent. The Vogt palisades composed of 7-10 layers of epithelial cells were demonstrated. The pupil was horizontally ovoid at rest (post-mortem). The sphincter pupil and the dilator pupil were well developed. Macroscopically, the tapetum lucidum appeared as a milky, non-opalescent crescent. Histologically, the choroidal tapetum lucidum cellulosum consisted of 5 to 9 layers of loosely packed oval cells. The retina showed a composition similar to that of terrestrial nocturnal carnivores. CONCLUSIONS: The results of our research indicate that the anatomical features of the eye and orbital region in the red panda share similarities with those described in the Musteloidea clade, as well as the Canidae and Ursidae families.

The organ of vision morphology of the southern two-toed sloth (Choloepus didactylus Linnaeus, 1758; Pilosa, Choloepodidae).

The Choloepus didactylus is a species belonging to the Choloepodidae family of the Folivora suborder. This study concerns the anatomical, morphometrical, histological and histochemical examination of the eye tunics, eyelids and orbital glands in the three adult Choloepus didactylus coming from the Wroclaw Zoological Garden. The cornea consists of four layers since Bowman's membrane was absent. The presence of palisades of Vogt formed from 14/15 to 20/21 layers of epithelial cells was demonstrated. Macroscopically, the tapetum lucidum was not identified, while degenerative choroidal tapetum lucidum cellulosum was found microscopically. The ciliary muscle was found with our histological approach. The pupil was round in shape (post-mortem). The presence of tarsal glands has not been demonstrated in both eyelids. The conjunctiva-associated lymphoid tissue (CALT) has been shown in the upper and lower eyelids. The superficial gland of the third eyelid produced a seromucous secretion. The third eyelid was shaped like a curved arch and composed of hyaline cartilage. The deep gland of the third eyelid was big and multilobar acinar simple with serous character. The lacrimal gland produced mucous secretion. The results of our research indicate that the features of the eye anatomy in the southern two-toed sloth (C. didactylus) are also typical of the Folivora suborder and have common features and differences with Vermilingua.