RESUMO
Nuclease-resistant moenomycin-binding aptamers with dissociation constants in the range of 300 to 400 nM have been selected. Competition experiments have demonstrated that these aptamers recognize a disaccharide analogue of moenomycin. The results offer the opportunity of setting up a selective and sensitive assay for identifying moenomycin biosynthetic precursors.
Assuntos
Antibacterianos/farmacologia , Bambermicinas/farmacologia , RNA/metabolismo , Antibacterianos/análise , Antibacterianos/química , Bactérias/crescimento & desenvolvimento , Técnicas Bacteriológicas , Bambermicinas/química , Sequência de Bases , Cromatografia Líquida de Alta Pressão , Técnicas de Química Combinatória , DNA/biossíntese , Dissacarídeos/química , Resistência Microbiana a Medicamentos , Testes de Sensibilidade Microbiana , Dados de Sequência Molecular , Estrutura Molecular , Oligonucleotídeos/química , Oligonucleotídeos/metabolismo , RNA/biossíntese , RNA/química , Análise de Sequência de RNARESUMO
A number of new moenomycin A derivatives have been prepared. Their antibiotic properties highlight the very specific recognition of moenomycin A at the transglycosylase binding site which is the basis of the transglycosylase inhibiting property of moenomycin A (4a).
Assuntos
Antibacterianos/química , Bambermicinas/química , Bambermicinas/farmacologia , Glicosiltransferases/antagonistas & inibidoresRESUMO
The anchoring of moenomycin A (1) to the bacterial cell cytoplasmic membrane is essential for its biological activity. The first details of the strength of this interaction and the kinetics of the diffusion-mediated intervesicle transfer have been obtained by means of fluorescence spectroscopic methods using a coumarin-labeled moenomycin A derivative.
