[The use of a protoplast-generating method in selecting the lytic enzyme producer Streptomyces recifensis subsp. lyticus]. / Ispol'zovanie metoda protoplastirovaniia v selektsii produtsenta liticheskikh fermentov Streptomyces recifensis subsp. lyticus.

Optimal conditions for the formation of stable protoplasts of S. recifensis subsp. lyticus 2435 were defined and the use of the protoplasting procedure to increase the activity of the synthesis of extracellular enzymes was shown in principle possible. The level of the synthesis of glycosidases and lytic endopeptidases by the isolated variant II-29 was higher than that the initial strain by 40-45 per cent which was due to the enrichment of the population with more active variants.

[Intergeneric hydridization of the actinomycetes Streptoverticillium mycoheptinicum and Streptomyces coelicolor]. / Mezhrodovaia gibridizatsiia aktinomitsetov Streptoverticillium mycoheptinicum i Streptomyces coelicolor.

Intergeneric crossing of the actinomycetes Streptoverticillium mycoheptinicum and Streptomyces coelicolor yielded recombinants which were mostly nonviable and unstable despite a relatively high frequency (10(-2)-10(-4)) at which their colonies appeared. The rare viable and stable recombinants were prototrophs. The structure of antibiotics in the hybrid cell may be modified as follows from the differences in the antibiotic activity of the LIA-973 hybrid and the parent strains.