RESUMO
The dynamics of in vitro development of reconstructed embryos of cattle after various electrofusion times was studied. The in vitro mature oocytes without zona pellucida enucleated using the touch method were taken as cytoplasts. Fetal fibroblasts were used as the nuclei source. Approximately 40% of embryos activated between 3 and 3.2 hours after electrofusion developed to blastocysts. The effectiveness of in vitro cloned embryo development did not decrease when the time between electrofusion and activation was extended up to 4-5 hours. The pattern of more successful development of in vitro reconstructed embryos was found using enucleated oocytes, excreting the first polar body after 18 hours in comparison with oocytes matured in vitro afterwards, as cytoplasts.
Assuntos
Blastocisto , Clonagem de Organismos , Desenvolvimento Embrionário , Fibroblastos , Técnicas de Transferência Nuclear , Oócitos , Animais , Blastocisto/citologia , Bovinos , Fusão Celular , Citoplasma , Estimulação Elétrica , Fibroblastos/citologia , Técnicas In Vitro , Oócitos/citologiaAssuntos
Esclerose Múltipla/diagnóstico , Feminino , Humanos , Masculino , Métodos , Pessoa de Meia-IdadeRESUMO
The content of mineral salts in all diseases of the skeleton has greater or less deviations from the norm. The change of the mineral content can be visually determined in routine roentgenograms when the deviation is not less than 20-30% from normal. This estimation has a great share of subjectivism. The optical density of the bone in roentgenogram objectively shows the content of calcium salts and its structure. Under study was the influence of soft tissues surrounding the lumbar part of the vertebral column upon the optical density index. Ten preparations of lumbar divisions by means of the aluminium wedge.
