[Mechanism of the interaction of chloramine with Pseudomonas aeruginosa]. / K mekhanizmu vzaimodeistviia khloramina s Pseudomonas aeruginosa.

The disinfecting effect of chloramine on P. aeruginosa depends on the concentration of this disinfecting agent, the concentration of P. aeruginosa and the period of exposure, with consideration for the time of attaining equilibrium between the concentration of chloramine and P. aeruginosa, affinity between the disinfectant and bacterial cells, the number of chloramine-binding sites on a bacterial cell, the admixture of organic substances, pH and other environmental characteristics. The interaction of chloramine and P. aeruginosa can be subdivided into two stages: (1) the binding of the disinfectant by bacterial cells and (2) the death of the microbes. The mathematical description of the process of disinfection requires that the main characteristics be expressed in molecular parameters and the time factor be correlated with the terms of attaining the equilibrium for free and bound chloramine. The average number of binding sites for chloramine on a bacterial cell and the affinity constant of chloramine to binding sites have been estimated.

[Results of the comparative use of virological and bacteriological control of routine disinfection in maternity homes]. / Rezul'taty sravnitel'nogo primeneniia virusologicheskogo i bakteriologicheskogo kontrolia tekushchei dezinfektsii v rodil'nykh domakh.

Bacterial viruses were often detected in washings from different environmental objects; not infrequently, the inoculation of washings from the same objects resulted in the growth of Escherichia coli. The expediency of making parallel tests of washings from these objects for the presence of wild strains of bacterial and viral cultures both during the control of disinfection measures and the determination of the effectiveness of disinfection agents is shown.

[Serological method of detecting the antigens of the causative agent of Sonne dysentery in lysates of the fecal inoculates from patients]. / Serologicheskii metod obnaruzhenii antigenov vozbuditelia dizenterii Zonne v lizatakh posevov isprazhenii bol'nykh.

Serological method of detection of Sh. sonnei antigens in the lysates of the patients, fecal cultures is suggested and approved. In the majority of cases of the results of bacteriological and serological methods of study of the feces coincided. Data confirming the specificity of the antibody neutralization test (ANT) in Sonne dysentery are presented. In connection with detection of the screening action of the Vi-antigen of typhoid bacilli there were elaborated additional methods for verifying the specificity of the ANT results. It is recommended to keep agar plates after selection of suspicious colonies during the bacteriological test; the lysate of the microbial crop should be additionally subjected to the ANT, this considerably increasing the percentage of laboratory confirmations of dysentery caused by Sh. sonnei.

[Utilization of a solid nutrient medium for the serological method of detecting the causative agent of typhoid in patients' feces]. / Ispol'zovanie tverdoi pitatel'noi sredy dlia serologicheskogo metoda obnaruzheniia vozbuditelia briushnogo tifa v isprazhneniiakh bol'nykh

The causative agent of typhoid fever can be revealed in feces of patients not only by the bacteriological, but also by the serological method, if the material is preliminarily grown on hard nutrient media; Ploskirev's medium proved to be the optimal. It is suggested that microbial cultures left on the ager in Petri dishes after the bacteriological study should be used for bacteriological analysis. In a number of cases it was possible to reveal the causative agent of typhoid fever in feces by the serological method, although no coproculture was isolated bacteriologically.