RESUMO
We analyzed IS6110-associated polymorphisms in the phospholipase C genes of 107 isolates of Mycobacterium tuberculosis selected to be representative of isolates circulating in central Russia. We found that the majority of Latin American-Mediterranean family strains contained an insertion in a unique position in the plcA gene, suggesting a common ancestor. This insertion can serve as a specific genetic marker for this group, which we designate the LAM-RUS family.
Assuntos
Mycobacterium tuberculosis/classificação , Mycobacterium tuberculosis/genética , Polimorfismo Genético , Tuberculose/microbiologia , Proteínas de Bactérias , Análise por Conglomerados , Elementos de DNA Transponíveis/genética , DNA Bacteriano/genética , Humanos , Mycobacterium tuberculosis/isolamento & purificação , Filogenia , Federação Russa/epidemiologia , Homologia de Sequência , Tuberculose/epidemiologia , Fosfolipases Tipo CRESUMO
The genotypic characteristics and drug susceptibility profiles of clinical isolates of Mycobacterium tuberculosis recovered from prison hospital patients in the Tula region (central Russia) during 2001 and 2002 are reported. The emergence of multi-drug-resistant tuberculosis (TB) poses a major health risk to the population, with economic implications for TB control. Prisons serve as a continuous source of TB transmission. The results showed that members of the LAM and Beijing families are major contributors to the epidemiological picture of TB in the population studied. The two families of strains accounted for most of the drug-resistant TB in the population. The genotypic characteristics of the M. tuberculosis predominant LAM strain that was responsible for 31 % of TB cases in this setting are presented.
Assuntos
Antibióticos Antituberculose/farmacologia , Isoniazida/farmacologia , Mycobacterium tuberculosis/efeitos dos fármacos , Mycobacterium tuberculosis/genética , Rifampina/farmacologia , Tuberculose Resistente a Múltiplos Medicamentos/epidemiologia , Tuberculose/epidemiologia , Adulto , Técnicas de Tipagem Bacteriana , Elementos de DNA Transponíveis/genética , Farmacorresistência Bacteriana Múltipla , Hospitais Especializados , Humanos , Masculino , Pessoa de Meia-Idade , Mycobacterium tuberculosis/classificação , Polimorfismo de Fragmento de Restrição , Prisioneiros , Fatores de Risco , Federação Russa/epidemiologia , Especificidade da EspécieRESUMO
We applied an enhanced version of subtractive hybridization for comparative analyses of indel differences between genomes of several Mycobacterium tuberculosis strains widespread in Russian regions, and the H37Rv reference strain. A number of differences were detected and partially analyzed, thus demonstrating the practicality of the approach. A majority of the insertions found were shared by all Russian strains, except for strain 1540 that revealed the highest virulence in animal tests. This strain possesses a number of genes absent from other clinical strains. Two of the differential genes were found to encode putative membrane proteins and are presumed to affect mycobacterial interaction with the host cell, thus enhancing virulent properties of the isolate. The method used is of general application, and enables the elaboration of a catalogue of indel polymorphic genomic differences between closely related strains.
