RESUMO
Blastocystis is a common enteric protozoan of humans and various species of animals. Culture and microscopic examination of fecal samples is the conventional method for identifying four major forms of Blastocystis (vacuolar, granular, non-vacuolar or cystic). In this article, we com- pared eight liquid media for cultivation of Blastocystis spp. Study material included fecal samples from clinically healthy pigs. Significant differences in the growth of Blastocystis on individual media were observed.
Assuntos
Blastocystis/fisiologia , Meios de Cultura , Animais , Fezes/parasitologia , Humanos , Suínos/parasitologiaRESUMO
A method is presented for rapid determination of bromide ion in commodities and blood by paired-ion liquid chromatography with electrochemical detection. The method involves extraction of samples with water and filtration. Blood is passed through a Sep-Pak C18 minicolumn. Recoveries are usually close to 100%, with satisfactory precision. The detection limit is 1 mg/kg. The method needs little labor and uses no noxious solvents or reagents.
Assuntos
Brometos/análise , Cromatografia Líquida/métodos , Grão Comestível/química , Frutas/química , Verduras/química , Brometos/sangue , Eletroquímica , Reprodutibilidade dos TestesAssuntos
Encefalopatias/cirurgia , Morte Súbita/etiologia , Complicações Pós-Operatórias/mortalidade , Embolia Pulmonar/mortalidade , Neoplasias da Medula Espinal/cirurgia , Adulto , Idoso , Encefalopatias/sangue , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Complicações Pós-Operatórias/etiologia , Embolia Pulmonar/etiologia , Neoplasias da Medula Espinal/sangueRESUMO
Chemiluminescence (CL) that appears during oxidation of lecithin and ascorbate has been studied. A simple system consisting only of purified lecithin, which has one double bond, and ascorbate as a physiological reductant with a low redox potential, was used. The CL spectrum of lecithin contain a strong band lying in the near infrared, and three bands at 20 900 cm-1, 17 700 cm-1 and 15 800 cm-1, being characteristic of singlet molecular oxygen (1O2). The effect of 1O2 quenchers on both autooxidation processes has also been investigated. The obtained results indicate that the main emitter is the 1O2. An addition of ascorbate to the system lecithin plus buffer causes a decrease of CL intensity. That is a result of stronger quenching properties of ascorbate and not due to efficiency of the generation of 1O2.
