[Clinical immunological characteristics of the pathogenesis of catarrhal gingivitis (Communication 1)]. / Kliniko-immunologicheskie osobennosti patogeneza kataral'nogog gingivita (Soobshchenie 1).

In order to evaluate the relationship between cellular and humoral immunity, washings from the gingival sulcus were analyzed in 15 patients with catarrhal gingivitis and 15 subjects with intact periodontium aged 19-22 years. The results indicate a close relationship between local immunity and humoral and cellular defense factors. It seems that decreased IgA level, complement component, and increased activities of C-reactive protein and antistreptolysin-O are the key factors in the development of chronic gingivitis. Permanent bacterial aggression leads to dysfunction of phagocytic and epithelial cells.

[Morphometric analysis of immunologic deficiency in newborn mice developing after transplacental transmission of live or inactivated influenza A virus]. / Morfometricheskii analiz immunologicheskoi nedostatochnosti u myshat, razvivaiushcheisia v rezul'tate transplatsentarnoi peredachi zhivogo ili inaktivirovannogo virusa grippa.

Morphometric evaluation of the thymus and spleen was used to characterize the complex immunological deficiency in suckling mice developing as a result of transplacental transmission of both live and inactivated influenza virus.

[Effect of T-activin on peripheral immunity organs in intact and thymectomized mice]. / Vliianie T-aktivina na perifericheskie organy immuniteta intaktnykh i timéktomirovannykh myshei.

Morphometry of the spleen, axillary lymph nodes and cytological assay of the bone marrow and peripheral blood were performed in (CBA X C57BL)F1 mice 1, 5, 10 and 15 days after subcutaneous injection of 0.5 microgram T-activin to intact and thymectomized (when adult) mice 2 months after operation. It was demonstrated that in intact animals, injection of T-activin stimulated the whole system of immunogenesis. The time course of plasmatization and the response of the germinative centers differing from that seen during antigen administration suggests that T-activin is not immunogenous, acting as a stimulant of the previous immune responses. The permanent amount of the degenerating cells attests to the lack of the toxic drug effect.

[Relationship between wound healing and the state of the immune system]. / Zavisimost' zazhivleniia rany ot sostoianiia immunnoi sistemy.

Principal differences in wound healing were established in rabbits with weak and strong delayed type hypersensitivity (DTH) to phytohemagglutinin (PHA). The evaluation included the rate of contraction of the wound defect, macroscopic control, cytology of the wound exudate, qualitative and quantitative histology. The rate of healing in strong DTH to PHA is 1 1/2 times as high and the death-rate of wound infection 2 times as low as in weak DTH. The response of immunity organs (thymus, spleen, regional lymph node) and peripheral blood leukocytes was also found to differ significantly despite their initial structural homogeneity. Comparison of the experimental data with the results of a similar study of selective therapeutic effect of PHA on wound healing in low DTH leads to a conclusion of the greatest intactness of the monocytic macrophage system, its leading role in correction of healing, as well as of the greatest defects in the neutrophil system.

[Effect of tetracycline on the immune and hematopoietic systems of intact rabbits]. / Vliiani tetratsiklina na immunnuiu i krovetvornuiu sistemy intaktnykh krolikov.

Tetracycline was administered orally in daily doses of 20 or 40 mg to 50 practically healthy male chinchilla rabbits weighing 2-2.5 kg. The thymus, appendage, spleen, ileocecal, mesenteric and axillary lymph nodes, bone marrow and peripheral blood were examined with the methods of morpho- cytometry. To verify the data the nonparametric criterion U of Wilcoxon-Mann-Whitny and the Student criterion were used. The mean curves reflecting the reaction level in the peripheral immunity system as a whole were plotted with the use of graphical analysis. It was found that tetracycline in doses of 20 and 40 mg induced a significant change in both the central and the peripheral immunity organs. Two trends in the development of the reaction in the system of immunogenesis were observed: (a) activation, transformation and differentiation of immunocompetent cells and (b) destruction of the cells, particular by transforming and proliferating cells. This gave rise to the depletion of the immunity system which was intensified if the drug was used for a prolonged period of time and in higher doses.

[Dynamics of structural changes in the organs of immunity of rabbits with acute experimental appendicitis]. / Dinamika strukturnykh i zmenenii organov immuniteta krolikov pri ostrom éksperimental'nom appenditsite.

Cyto- and histological analysis of the whole immunogenic system at the development of an acute experimental appendicitis in rabbits has demonstrated that all the constituent parts of the system are involved into the reaction. In the central organ of immunity--thymus the main change is its progressive exhaustion, while peripheral organs of immunity are subjected to rather essential cytological rearrangements and activation of both cellular and humoral immunity. Accordingly to the type of the reaction, two groups of peripheral organs of immunity can be distinguish: the first--the appendix and the iliocecal lymph node which predominantly participate in the immunoblastic reaction and in the reaction of young plasmatic cells and demonstrate increased signs of immature cells emigration; the second--the axillary lymph node, as a representative of somatic nodes, and the spleen--the main carriers of the reaction in mature plasmic cells. Structural peculiarities of the complex appendix--iliocecal lymph node in intact animals and in the experiment make it possible to consider the complex as a peculiar functional link of the immune peripheral organs.

[Effect of therapeutic doses of tetracycline on the structure of the thymus gland in rabbits]. / Vliianie terapevticheskikh doz tetratsiklina na strukturu vilochkovoi zhelezy u krolikov.

Tetracycline was administered orally to Shinshilla male rabbits (female, 2.0-2.5 kg) twice a day, the daily dose being 20 mg. The thymus was examined in 1 and 2 weeks by the methods of histo- and cytometry. The reliability of the differences and correlation were estimated by the non-parametric methods. Out of 15 parameters studied 8 showed reliable differences and 5 were statistically connected. The following structural shifts were found: (1) increased numbers of activated and middle thymocytes evident of intraorgan cell activation, (2) higher mitotic index and lower index of the cell degradation resulting in the cortex hypertrophia by the end of the week, (3) increased cell density in the medullar substance which is the morphological criterion of intraorgan migration of the cells from the cortex into the medullar substance. Thus, the morphometric analysis of the thymus proved to be much more informative than the usual morphological analysis. The observed structural change in the thymus is considered to be a result of the antigenic effect of tetracycline on the host.

[Refringence phenomenon as a sign of the degree of differentiation of immunocompetent cells]. / Fenomen prelomleniia kak priznak stepeni differentsirovki immunokompetentnykh kletok

Quantitative and qualitative analyses of the refringence phenomen in immunocompetent cells of various organs of rabbits and CBA line mice under conditions of immunological activity, as well as in those of children with ataxia-teleangiectasia, showed that immunoblasts contained predominantly non-refringent nuclei, mature plasmatic cells--mainly highly refringent nuclei, and young plasmatic cells occupied a position in the middle of the two. These data, as well as the absence of the refringence phenomenon in the cells of lymphosarcoma in man corroborate a direct relationship between the phenomenon and the process of differentiation of cells.