Chaperone activity in the lens.

INTRODUCTION: alpha-crystallin, the major protein of the eye lens, is a molecular chaperone that is able to prevent the precipitation of denatured proteins. This activity is thought to be important for the maintenance of lens transparency. Loss of the activity has been postulated to contribute to the development of cataract. The purpose of this study was to determine how chaperone activity was affected by growth and ageing of the lens. METHODS: alpha-crystallins were purified from nine concentric tissue layers removed from an adult bovine lens. The ability to inhibit the precipitation of beta(L)-crystallin, following thermal denaturation, was used to assess the chaperone activity of these proteins. The molar ratio of alpha-crystallin/beta(L)-crystallin required to inhibit the precipitation of beta(L)-crystallin by 50 per cent was used as a measure of the affinity of the chaperone for denatured protein. RESULTS: As evidenced by a gradual increase in the ratio, from 0.52 to 1.24, the protective ability of alpha-crystallin decreased from the outside of the lens into the centre. alpha-crystallin from the cortex of the lens provided greater protection against precipitation of proteins than older alpha-crystallin from the nucleus. The reasons for this were investigated. Gel electrophoresis of the proteins from each concentric layer revealed an increase in degraded polypeptides from approximately one per cent in the cortex to more than nine per cent in the centre of the lens. This increase appears to be correlated with the decrease in chaperone ability. Renaturing alpha-crystallin obtained from the nucleus did not increase its chaperone activity, indicating conformational changes were not responsible for the decreased activity. Phosphorylation did not appear to have any significant effect on the chaperone activity. CONCLUSION: The loss of chaperone activity, accompanying fibre cell compression into the centre of the lens, can be attributed to degradation of the alpha-crystallin polypeptides.

Considerations in the routine assessment and treatment of anisometropic amblyopia.

Anisometropic amblyopia has traditionally been treated by direct occlusion with or without refractive correction of the anisometropia. This treatment has generally been considered so successful that practitioners prescribe occlusion for a time and consider treatment complete when visual acuity is no longer improving. The aetiology of anisometropic amblyopia, addressing the functional deficits of the amblyopic system and patient compliance issues, are important to the success of amblyopia treatment in anisometropia and are briefly reviewed in this paper. We propose that, while each case of anisometropic amblyopia may have slightly different attributes, initially they should all be treated with occlusion and full-time spectacle correction. Some presentations may be responsive to individual treatment strategies to correct binocular functions such as stereopsis, accommodative response and fusional vergences.

A review of current research on the effect of diabetes mellitus on the eye.

It is estimated that almost one million Australians will have diabetes by the year 2000. Of those with diabetes a significant proportion will have eye-related conditions, the most debilitating being diabetic retinopathy. Appropriate identification and treatment can result in prevention of visual loss and blindness. The importance of diabetes as a cause of blindness in our community is realised by the commencement of a national program by the National Health and Medical Research Council to develop clinical practice guidelines for the management of diabetic retinopathy. The development of these guidelines was based on available evidence following an extensive review of the literature up to May 1996. This review is a summary of our advances in research on the effect of diabetes on various aspects of the eye and vision over the past two years. This review is a compilation of articles of research on the effect of diabetes on various aspects of the eye and vision. As a result of the enormous amount of effort and work by scientists and clinicians around the world, as well as space restrictions, the review covers the past two years only. Although every effort has been made to include as many research articles as possible, not all articles of research are covered. It is intended that this review provide an overview of the latest trends in research, particularly relating to new techniques and methods in the study of diabetes in ocular tissue as well as the new theories in the development of ocular damage to each of the tissue.