RESUMO
This paper explores the impacts that the New Zealand government's lockdown measures to contain the COVID-19 virus during 2020 had on the activity and experiences of outdoor recreationists in New Zealand. Concepts related to coping strategies such as rationalisation, displacement and substitution, have been used frequently to explain the behavioural changes and processes involved in outdoor recreation when disruptions arise such as crowding and recreational conflict. However, such concepts have rarely been applied to rapid on-set disruptors such as pandemics. This paper adopts coping strategy theory to help document the strategies adopted by outdoor recreationists in response to the national lockdown in 2020. Based on a qualitative analysis of twenty interviews with outdoor recreationists in New Zealand, various coping mechanisms such as temporal, activity, and spatial displacement are identified. These include increased appreciation for outdoor settings, discovery of local activities and microadventures, and increased walking activity across the restricted, reaction and reset periods. Outdoor recreation coping strategies may contribute to increased resilience to disruptive and rapid on-set events and enhance understanding of how recreationists respond and adapt to disruption. This research presents a unique insight of coping strategies adopted in response to the national lockdown that may have implications for participation and management of outdoor recreation in New Zealand in the coming years. This paper also offers a new perspective on the behaviourist tradition in the field of outdoor recreation which may be fruitful for future research examining rapid on-set disruptions and crises.
RESUMO
Solvent-assisted protein digestion involves enzymatic hydrolysis in mixed aqueous-organic solvents. With trypsin, acetonitrile is the modifying solvent of choice, recommended at concentrations from 10 to 80% to improve protein sequence coverage in mass spectrometry. Spectroscopic activity assays employing substrate mimics such as N-benzoyl arginine ethyl ester (BAEE) appear to show a relative enhancement of trypsin activity in mixed solvent systems. However, as reported here, the changing solvent polarity induces bias in the absorbance measurement, lending upward of 35% error in the apparent trypsin activity as the acetonitrile is raised to 70%. Furthermore, time-dependent spectroscopic and mass spectrometric measurements reveal a progressive deactivation of trypsin over a 5- to 10-min period in as little as 30% acetonitrile.
