RESUMO
Analysis of restriction fragment length polymorphism (RFLP) in mitochondrial DNA (mtDNA) in a population of Leptothorax acervorum demonstrates substantial population substructuring. Digestion with four restriction endonucleases, HaeIII, MboI, MspI and RsaI, gave six, four, three and two different patterns, respectively. Seven composite haplotypes were obtained from the observed cleavage patterns. Uniform aggregations of nests from the same maternal lineage (i.e. with the same haplotype) were found which suggests that nest-founding by a process of budding-off is common. Groups of nests seem to actively exclude other haplotypes from establishing nests within their territory. F-statistics suggests that individuals mate at random within the population.
Assuntos
Formigas/genética , DNA Mitocondrial/genética , Herança Extracromossômica , Polimorfismo de Fragmento de Restrição , Animais , Cruzamento , Haplótipos , SuéciaRESUMO
A glutathione transferase from human mononuclear leukocytes with a high activity toward trans-stilbene oxide (GT-tSBO) has been studied in liver and blood from fetus and adults and in blood from neonates. Using starch gel electrophoresis, different phenotypes of GST1 have been determined, GST1 0, GST1 1, and GST1 2. As judged from activity measurements and the fact that only those individuals who express the null allele of GST1, the GST1 0, which has a low activity toward trans-stilbene oxide, it is concluded that the hepatic transferase GST1 is identical to GT-tSBO, as well as to hepatic transferase mu. In addition, it has been shown that the different genotypes of GST1 1 (GST1 1-1, GST1 1-0) and GST1 2 (GST1 2-2, GST1 2-0) can be separated by measuring the GT-tSBO activity in whole blood from the same individual. It is also demonstrated that GT-tSBO activity is much lower in fetal liver, approximately 10 times, compared with adult liver, while this activity seems to be unchanged in the blood from fetus and adults, as well as in neonates.