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1.
Water Sci Technol ; 59(10): 2009-15, 2009.
Artigo em Inglês | MEDLINE | ID: mdl-19474496

RESUMO

In this paper, the influence of the polymer-induced flocculation on undigested sludge dewatering is investigated by image analysis. The mixing of flocculants in a so-called rapid-mixer and the subsequent forming of aggregates is carried out by the FlocFormer system, a new sludge conditioning system. For the mechanical dewatering a decanter device is used. To evaluate the efficiency of the dewatering process, the total solid content (TS) of the dewatered sludge is analysed. The results of this study show that this type of flocculation represents an alternative to improve sludge dewatering of undigested sludge. Compared with the standard conditioning (T-mixer), the FlocFormer leads to a better improvement (1.37 to 3.46%-points) in terms of TS while the rapid-mixer improves the dewatering efficiency only by 0.50 to 1.73%-points. Additionally, by using the FlocFormer conditioning, an optimal polymer dose of 5 g/kg TS has been detected. By increasing the polymer consumption (4-20% above the optimal dose) the dewatering efficiency remains practically constant (TS approximately 26.5%).


Assuntos
Esgotos/análise , Eliminação de Resíduos Líquidos/métodos , Desenho de Equipamento , Floculação , Resíduos Industriais , Cinética , Tamanho da Partícula , Eliminação de Resíduos Líquidos/instrumentação , Purificação da Água
2.
Am J Physiol Heart Circ Physiol ; 280(6): H2770-8, 2001 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-11356635

RESUMO

Mitochondria contribute to myocyte injury during ischemia. After 30 and 45 min of ischemia in the isolated perfused rabbit heart, subsarcolemmal mitochondria (SSM), located beneath the plasma membrane, sustain a decrease in oxidative phosphorylation through cytochrome oxidase. In contrast, oxidation through cytochrome oxidase in interfibrillar mitochondria (IFM), located between the myofibrils, remains unaffected. Cytochrome oxidase activity in the intact membrane requires an inner mitochondrial membrane lipid environment enriched in cardiolipin. During ischemia, the content of cardiolipin decreased only in SSM, whereas the content of other phospholipids was preserved. Ischemia did not alter the composition of the cardiolipin that remained in SSM. Cardiolipin content was preserved in IFM during ischemia. Thus cardiolipin is a relatively early target of ischemic mitochondrial damage, leading to loss of oxidative phosphorylation through cytochrome oxidase in SSM.


Assuntos
Cardiolipinas/metabolismo , Mitocôndrias Cardíacas/metabolismo , Isquemia Miocárdica/metabolismo , Miocárdio/metabolismo , Sarcolema/metabolismo , Animais , Cardiolipinas/análise , Cromatografia Líquida de Alta Pressão , Grupo dos Citocromos c/análise , Grupo dos Citocromos c/metabolismo , Modelos Animais de Doenças , Miocárdio/química , Miofibrilas/metabolismo , Fosforilação Oxidativa , Fosfolipídeos/análise , Fosfolipídeos/metabolismo , Coelhos
3.
Anal Biochem ; 285(2): 246-54, 2000 Oct 15.
Artigo em Inglês | MEDLINE | ID: mdl-11017709

RESUMO

We describe a comprehensive approach to the separation, quantitation, and characterization of phospholipids and lysophospholipids present in complex biological samples. The central feature is a normal-phase HPLC separation of individual phospholipid and lysophospholipid classes. In this single chromatographic step, phospholipids and lysophospholipids are separated and recovered for quantitation by organic phosphate assay and characterization by acyl-group composition. Recovery of phospholipids and lysophospholipids from HPLC averages 80-90%. Isolated phospholipid and lysophospholipid fractions are available for separation of individual molecular species by second-dimension reverse-phase HPLC and characterization of individual molecular species by mass spectrometry.


Assuntos
Cromatografia Líquida de Alta Pressão/métodos , Lisofosfolipídeos/análise , Fosfolipídeos/análise , Fósforo/análise , Animais , Cromatografia em Camada Fina , Estudos de Avaliação como Assunto , Lisofosfolipídeos/isolamento & purificação , Espectrometria de Massas , Fosfolipídeos/isolamento & purificação , Coelhos
4.
Eur J Biochem ; 267(6): 1795-804, 2000 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-10712612

RESUMO

A second generation of lipid-linked oligosaccharide probes, fluorescent neoglycolipids, has been designed and synthesized for ligand discovery within highly complex mixtures of oligosaccharides. The aminolipid 1,2-dihexadecyl-sn-glycero-3-phosphoethanolamine (DHPE), which has been used extensively to generate neoglycolipids for biological and structural studies, has been modified to incorporate a fluorescent label, anthracene. This new lipid reagent, N-aminoacetyl-N-(9-anthracenylmethyl)-1, 2-dihexadecyl-sn-glycero-3-phosphoethanolamine (ADHP), synthesized from anthracenaldehyde and DHPE gives an intense fluorescence under UV light. Fluorescent neoglycolipids derived from a variety of neutral and acidic oligosaccharides by conjugation to ADHP, by reductive amination, can be detected and quantified by spectrophotometry and scanning densitometry, and resolved by TLC and HPLC with subpicomole detection. Antigenicities of the ADHP-neoglycolipids are well retained, and picomole levels can be detected using monoclonal carbohydrate sequence-specific antibodies. Among O-glycans from an ovarian cystadenoma mucin, isomeric oligosaccharide sequences, sialyl-Lea- and sialyl-Lex-active, could be resolved by HPLC as fluorescent neoglycolipids, and sequenced by liquid secondary-ion mass spectrometry. Thus the neoglycolipid technology now uniquely combines high sensitivity of immuno-detection with a comparable sensitivity of chemical detection. Principles are thus established for a streamlined technology whereby an oligosaccharide population is carried through ligand detection and ligand isolation steps, and sequence determination by mass spectrometry, enzymatic sequencing and other state-of-the-art technologies for carbohydrate analysis.


Assuntos
Corantes Fluorescentes/química , Fluorometria/métodos , Glicolipídeos/análise , Oligossacarídeos/química , Fosfatidiletanolaminas/química , Aminação , Sequência de Carboidratos , Cromatografia Líquida de Alta Pressão , Cromatografia em Camada Fina , Cistadenocarcinoma/química , Cistadenocarcinoma/patologia , Feminino , Glicolipídeos/isolamento & purificação , Humanos , Ligantes , Espectrometria de Massas , Dados de Sequência Molecular , Mucinas/química , Proteínas de Neoplasias/química , Neoplasias Ovarianas/química , Neoplasias Ovarianas/patologia , Fosfatidiletanolaminas/síntese química , Polissacarídeos/química , Polissacarídeos/isolamento & purificação , Sensibilidade e Especificidade
5.
J Immunol Methods ; 227(1-2): 109-19, 1999 Jul 30.
Artigo em Inglês | MEDLINE | ID: mdl-10485259

RESUMO

This study was aimed at investigating the efficacy of presentation of biotinylated oligosaccharides on streptavidin-coated microwells for interactions with (a) three monoclonal antibodies directed at sialyl-Lewisa (Le(a)) or sulfo-Le(a)-related sequences, and (b) the endothelium-leukocyte adhesion molecules, the E-, L- and P-selectins which recognize both the sulfo- and sialyl-Le(a) series. With the antibodies it was observed that if the biotinylated oligosaccharide incorporated the entire antigenic determinant, and additional saccharide length was not included, the biotinyl tag spacer length was a critical factor in the strength of the binding signal. If oligosaccharide chain beyond the determinant was included, the biotinyl tag spacer length was less important. The E-selectin binding data with the biotinylated sialyl- and sulfo-oligosaccharides were in overall accord with previous knowledge. With the L- and P-selectins, however, unexpectedly low binding signals were elicited by biotinyl sulfo-Le(a) sequences relative to those with the sialyl-analogs. This suppression was more pronounced with the rodent than the human L-selectin. Such differential availabilities of oligosaccharides displayed on streptavidin may relate to biological situations, such as the differential reactivities of the three selectins with a given oligosaccharide ligand presented on different carrier proteins, or on different O-glycan cores on mucin-type glycoproteins.


Assuntos
Anticorpos Monoclonais/imunologia , Oligossacarídeos/imunologia , Selectinas/imunologia , Animais , Biotina , Humanos , Camundongos
6.
Glycobiology ; 8(3): 227-36, 1998 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-9451032

RESUMO

Biotinyl-oligosaccharides are a relatively new generation of saccharide probes that enable immobilization of desired oligosaccharides on streptavidin matrices for studies of carbohydrate-protein interactions. Here we describe the facile preparation of biotinyl-l-3-(2-naphthyl)-alanine hydrazide (BNAH) derivatives of oligosaccharides, containing a strong UV absorbing and fluorescent group, in which the ring of the reducing-end monosaccharide is nonreduced. We evaluate reactivities of immobilized BNAH- N -glycans with plant lectins that recognize aspects of the oligosaccharide core or outer-arms. We make some comparisons with 2-amino-6-amidobiotinyl-pyridine (BAP) derivatives obtained by reductive amination, and 6-(biotinyl)-aminocaproyl-hydrazide (BACH) derivatives which have a longer spacer-arm. N -Glycan-BNAH and-BAP derivatives have, overall, comparable reactivities with lectins which recognize N -glycan outer-arms or the trimannosyl core, but only BNAH and BACH derivatives are bound by lectins which recognize the non-reduced core. Moreover, with Pisum sativum agglutinin (PSA) which additionally requires the fucosyl- N- glycan-asparaginyl core for high affinity binding, the immobilized BNAH derivative (which is an alanine hydrazide beta-glycoside) can substitute for the natural beta-glycosylasparaginyl core, whereas the BACH derivative (aminocaproyl-hydrazide-beta-glycoside) is less effective. BNAH is a derivatization reagent of choice, therefore, for solid phase carbohydrate-binding experiments with immobilized N -glycans.


Assuntos
Corantes Fluorescentes/metabolismo , Lectinas/metabolismo , Oligossacarídeos/química , Aglutinação/efeitos dos fármacos , Alanina/análogos & derivados , Biotina/análogos & derivados , Configuração de Carboidratos , Sequência de Carboidratos , Eritrócitos/metabolismo , Humanos , Hidrazinas/química , Dados de Sequência Molecular , Estrutura Molecular , Naftalenos/química , Pisum sativum/química , Lectinas de Plantas , Proteínas de Plantas/metabolismo , Ligação Proteica , Estreptavidina/metabolismo
7.
Mol Biochem Parasitol ; 66(2): 319-28, 1994 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-7808481

RESUMO

Activation of complement on the surface of parasitic protozoa of the genus Leishmania appears to be important for parasite infectivity in the mammalian host, as it allows these parasites to attach to and invade macrophages via their surface complement receptors. Serum mannan-binding protein (MBP) is a known activator of complement. Therefore, in the present study, we have investigated whether serum MBP binds to live Leishmania parasites, and to mannose-containing saccharides derived from the parasite cell surface. We have observed by fluorescence microscopy that biotinylated MBP binds to the surface of L. major and L. mexicana promastigotes. At this developmental stage the parasites are coated by a mannose-containing lipophosphoglycan (LPG). We have observed that radioiodinated MBP binds in a mannose-inhibitable manner to purified LPG which has been immobilized in plastic microwells, as well as to purified mannose-terminating di-, tri- and tetrasaccharide fragments ('cap' structures) which have been released by mild acid hydrolysis from the outer chains of the LPG, converted into neoglycolipids and resolved by thin-layer chromatography. 125I-MBP also binds in the chromatogram-binding assay to the mannose-containing glycoinositol-phospholipids that are expressed in high copy number on both the promastigote and the intracellular amastigote stages of most Leishmania species. These data suggest that MBP has the potential to opsonize the major developmental stages of Leishmania parasites, and provide a possible mechanism for the antibody-independent activation of complement on their surface.


Assuntos
Proteínas de Transporte/sangue , Proteínas de Transporte/imunologia , Glicoconjugados/imunologia , Leishmania/imunologia , Mananas/metabolismo , Animais , Sequência de Carboidratos , Membrana Celular/imunologia , Colectinas , Ativação do Complemento , Glicoconjugados/química , Glicoesfingolipídeos/química , Glicoesfingolipídeos/imunologia , Humanos , Técnicas In Vitro , Leishmania/crescimento & desenvolvimento , Leishmania/patogenicidade , Leishmania major/imunologia , Leishmania mexicana/imunologia , Leishmaniose/etiologia , Dados de Sequência Molecular , Proteínas Opsonizantes/sangue , Fagocitose
9.
Eur J Biochem ; 217(2): 645-55, 1993 Oct 15.
Artigo em Inglês | MEDLINE | ID: mdl-7693465

RESUMO

Mucins from the pooled gastric juice of Lewis-positive secretors were investigated to establish their glycosylation patterns with particular reference to the type and abundance of the glycan-core structures. Following reductive beta-elimination, the neutral glycan alditols from these mucins were fractionated by ion exchange and size-exclusion chromatographies and subjected to structural analyses. It was possible to gain insights into the core sequences of the neutral O-linked glycan alditols by matching (a) composition data from liquid secondary-ion mass spectrometry of the native alditol fractions, (b) specific structural information on the core sequences by thin-layer-chromatography mass spectrometry of alditol-derived neoglycolipids and (c) data from electron-impact mass spectrometry of permethylated glycan alditols or their partially methylated alditol acetates. The predominant core structures detected among the neutral glycans representing about 77% (by mass) of the total carbohydrates released from gastric mucins were core 1, Gal beta 1-3GalNAc (Ac, acetyl) and core 2, Gal beta 1-3(GlcNAc beta 1-6)GalNAc in the approximate ratio 1:2. Core 3, GlcNAc beta 1-3GalNAc, and core 4, GlcNAc beta 1-3(GlcNAc beta 1-6)GalNAc, were much less abundant (< 10%), while core 5, GalNAc alpha 1-3GalNAc, core 6, GlcNAc beta 1-6GalNAc, and a recently described sequence GalNAc alpha 1-6GalNAc (core 7) were not detected. This investigation also addressed the question of the presence of the sequence Gal beta 1-6GalNAc which has been reported previously to occur as a core-structure element in gastric mucins. This was greatly assisted by the availability of the authentic chemically synthetized disaccharide alditol which, when converted into a neoglycolipid after mild periodate oxidation, gives diagnostic ions in mass spectrometry and can be detected with high sensitivity. No evidence was found for the presence of this unusual sequence among the oligosaccharides in gastric mucins.


Assuntos
Mucinas Gástricas/química , Oligossacarídeos/análise , Polissacarídeos/química , Sequência de Carboidratos , Cromatografia Líquida de Alta Pressão , Cromatografia em Camada Fina , Suco Gástrico/química , Glicolipídeos/química , Glicosilação , Humanos , Espectrometria de Massas , Metilação , Dados de Sequência Molecular , Álcoois Açúcares/química
10.
Carbohydr Res ; 239: 107-15, 1993 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-8384524

RESUMO

The specificity of mild periodate oxidation of 3- and 6-substituted 2-acetamido-2-deoxy-D-galactitols and 4- and 6-substituted D-glucitols has been investigated. The products were reacted with 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine and the derivatives were analysed by application of liquid secondary-ion mass spectrometry directly to the TLC plates. There was > 95% specificity of cleavage of the C-4-C-5 bond (threo-diol) for the GalNAcol derivatives. The major sites of oxidation for the Glcol derivatives also involved threo-diols. For alpha-Neu5Ac-(2-->6)-GalNAcol, approximately 30% of the products of oxidation involved the sialic acid side chain, and approximately 60% were cleaved at the C-4-C-5 bond of the GalNAcol moiety. The mild periodate oxidation reaction forms part of a strategy for determining the patterns of branching of the cores of O-linked glycoprotein oligosaccharides and other oligosaccharide-alditols.


Assuntos
Glicoproteínas/química , Oligossacarídeos/química , Álcoois Açúcares/química , Sequência de Carboidratos , Cromatografia em Camada Fina , Espectrometria de Massas , Dados de Sequência Molecular , Oxirredução , Ácido Periódico/química , Fosfatidiletanolaminas/química
11.
Infect Immun ; 60(12): 5078-84, 1992 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-1452340

RESUMO

Membrane glycolipids contain the lactose sequence (galactose linked to glucose), and the oligosaccharide is variously extended such that there is a cell-type-specific repertoire. In this study, binding of Pseudomonas aeruginosa M35 to lipid-linked lactose (Gal beta 1-4Glc [structure 1]), lacto-N-neotetraose (Gal beta 1-4GlcNAc beta 1-3Gal beta 1-4Glc [structure 2]), lacto-N-tetraose (Gal beta 1-3GlcNAc beta 1-3Gal beta 1-4Glc [structure 3]), and asialo GM1 (Gal beta 1-3GalNAc beta 1-4Gal beta 1-4Glc [structure 4]) was evaluated and compared with binding of Escherichia coli C600 to these compounds. Oligosaccharides were linked to the lipid phosphatidylethanolamine dipalmitoate, and the resulting neoglycolipids were resolved on thin-layer chromatograms or coated onto plastic microtiter wells. Lipid-linked structures 1 to 4 were bound by P. aeruginosa and E. coli in the chromatogram assay, but only structure 4 was bound in the microtiter well assay. As shown previously for E. coli binding to lipid-linked structures 1 to 3, binding to lipid-linked structure 4 was not inhibited with oligosaccharide, indicating a requirement for lipid and oligosaccharide. With few exceptions, sialylation and fucosylation of structures 1 to 4 resulted in impaired or abolished binding. Comparisons of binding intensities in the chromatogram assay indicated that recognition by P. aeruginosa and recognition by E. coli are not identical. Presence of the additional disaccharide unit, as in structure 2, resulted in enhanced binding of P. aeruginosa but diminished binding of E. coli relative to lactose binding; fucosylation at galactose of lactose resulted in markedly diminished binding of P. aeruginosa only. In the microtiter well assay, binding of E. coli to asialo GM1 was much weaker than P. aeruginosa binding. The saccharide-plus-lipid-dependent adhesion may be an important factor in increased susceptibility to infection of epithelia already damaged by microbial and chemical agents; the differing strengths of adhesion to the structural variants may relate to tissue tropism.


Assuntos
Escherichia coli/fisiologia , Glicolipídeos/metabolismo , Lactose/metabolismo , Oligossacarídeos/metabolismo , Pseudomonas aeruginosa/fisiologia , Aderência Bacteriana , Sequência de Carboidratos , Glicosilação , Leite Humano/metabolismo , Dados de Sequência Molecular
12.
Biochem Biophys Res Commun ; 188(1): 244-51, 1992 Oct 15.
Artigo em Inglês | MEDLINE | ID: mdl-1384480

RESUMO

The binding of the leucocyte adhesion molecule L-selectin has been investigated toward several structurally defined lipid-linked oligosaccharides immobilized on silica gel chromatograms or plastic wells. In both assay systems the 3'-sulphated Le(a)/Le(x) type tetrasaccharides [formula: see text] were more strongly bound than 3'-sialyl analogues. A considerable binding was observed to the 3'-sulphated oligosaccharide backbone in the absence of fucose but not to a 3'-sialyl analogue or fuco-oligosaccharide analogues lacking sulphate or sialic acid. Affinity for other sulphated saccharides: 3'-sulphoglucuronyl neolactotetraosyl ceramide and glycolipids with sulphate 3'-linked to terminal or sub-terminal galactose or N-acetylgalactosamine was detected in the chromatogram assay only. These studies, together with earlier reports that L-selectin binding to endothelium is inhibited by sulphatide, highlight the relative importance of sulphate in the adhesive specificity of this protein.


Assuntos
Moléculas de Adesão Celular/metabolismo , Antígenos do Grupo Sanguíneo de Lewis/metabolismo , Glicoproteínas de Membrana/metabolismo , Oligossacarídeos/metabolismo , Animais , Sequência de Carboidratos , Glicolipídeos/metabolismo , Glicoesfingolipídeos/metabolismo , Humanos , Imunoglobulina G/metabolismo , Cinética , Selectina L , Dados de Sequência Molecular , Oligossacarídeos/química , Ratos , Proteínas Recombinantes de Fusão/metabolismo , Relação Estrutura-Atividade
13.
Biochemistry ; 31(38): 9126-31, 1992 Sep 29.
Artigo em Inglês | MEDLINE | ID: mdl-1382586

RESUMO

E-selectin is the inducible adhesion protein on the surface of endothelial cells which has a crucial role in the initial stages of recruitment of leucocytes to sites of inflammation. In addition, it is almost certainly involved in tumor cell adhesion and metastasis. This report is concerned with identification of a new class of oligosaccharide ligand--sulfate-containing--for the human E-selectin molecule from among oligosaccharides on an ovarian cystadenoma glycoprotein. This has been achieved by application of the neoglycolipid technology to oligosaccharides released from the glycoprotein by mild alkaline beta-elimination. Oligosaccharides were conjugated to lipid, resolved by thin-layer chromatography, and tested for binding by Chinese hamster ovary cells which had been transfected to express the full-length E-selectin molecule. Several components with strong E-selectin binding activity were revealed among acidic oligosaccharides. The smallest among these was identified by liquid secondary ion mass spectrometric analysis of the neoglycolipid, in conjunction with methylation analysis of the purified oligosaccharide preparation as an equimolar mixture of the Le(a)- and Le(x)/SSEA-1-type fucotetrasaccharides sulfated at position 3 of outer galactose: [formula: see text] To our knowledge this is the first report of a sulfofucooligosaccharide ligand for E-selectin. The binding activity is substantially greater than those of lipid-linked Le(a) and Le(x)/SSEA-1 sequences and is at least equal to that of the 3'-sialyl-Le(x)/SSEA-1 glycolipid analogue.


Assuntos
Moléculas de Adesão Celular/metabolismo , Cistadenoma/química , Glicolipídeos/síntese química , Glicoproteínas de Membrana/química , Oligossacarídeos/química , Neoplasias Ovarianas/química , Ácidos Sulfúricos/análise , Configuração de Carboidratos , Sequência de Carboidratos , Selectina E , Feminino , Glicoesfingolipídeos/síntese química , Humanos , Ligantes , Espectrometria de Massas , Glicoproteínas de Membrana/isolamento & purificação , Glicoproteínas de Membrana/metabolismo , Modelos Moleculares , Dados de Sequência Molecular , Oligossacarídeos/isolamento & purificação
14.
J Biol Chem ; 267(19): 13661-8, 1992 Jul 05.
Artigo em Inglês | MEDLINE | ID: mdl-1377689

RESUMO

Carbohydrate recognition by the human endothelial-leukocyte adhesion molecule, E-selectin, has been investigated by binding studies using 3H-labeled Chinese hamster ovary cells expressing different levels of the transfected full-length adhesion molecule and a series of structurally defined oligosaccharides linked to the lipid phosphatidylethanolamine dipalmitoate (neoglycolipids) and synthetic glycolipids chromatographed on silica gel plates or immobilized on plastic wells. Evidence is presented for density-dependent binding of the membrane-associated E-selectin not only to 3'-sialyl-lacto-N-fucopentaose II (3'-S-LNFP-II) and 3'-sialyl-lacto-N-fucopentaose III (3'-S-LNFP-III) which express the sialyl Le(a) and sialyl Le(x) antigens, respectively, but also to the nonsialylated analogue LNFP-II; there is a threshold density of E-selectin required for binding to these sialylated sequences, and binding to the nonsialylated analogue is a property only of cells with the highest density of E-selectin expression. The presence of fucose linked to subterminal rather than to an internal N-acetylglucosamine is shown to be a requirement for E-selectin binding, and although the presence of sialic acid 3-linked to the terminal galactose of the LNFP-II or LNFP-III sequences substantially enhances E-selectin binding, the presence of 6-linked sialic acid abolishes binding. E-selectin binding is unaffected in the presence of the blood group H fucose (alpha 1-2 linked to galactose to form the Le(b) antigen). However, the binding is abolished when in addition alpha 1-3-linked N-acetylgalactosamine to the galactose (blood group A antigen) is present. These results indicate that some E-selectin-mediated adhesive events may be influenced by blood group status.


Assuntos
Metabolismo dos Carboidratos , Moléculas de Adesão Celular/metabolismo , Antígenos CD15/metabolismo , Oligossacarídeos/metabolismo , Animais , Células CHO , Sequência de Carboidratos , Cromatografia Líquida , Cromatografia em Camada Fina , Cricetinae , DNA , Selectina E , Camundongos , Dados de Sequência Molecular , Especificidade por Substrato
15.
Carbohydr Res ; 221: 191-208, 1991 Dec 16.
Artigo em Inglês | MEDLINE | ID: mdl-1726196

RESUMO

As part of the establishment of a data base for core and backbone sequences of O-linked oligosaccharides of human meconium glycoproteins, the minor tetra- to hepta-saccharides released from mild-acid treated blood group [corrected] H-active glycoproteins have been studied. These oligosaccharides are heterogeneous and difficult to isolate, and a t.l.c.-m.s. microsequencing procedure has been applied to the neoglycolipid derivatives, in conjunction with 1H-n.m.r. spectroscopy, methylation analysis, and mass spectrometry (m.s.) of native and methylated oligosaccharides. Among an array of oligosaccharides characterised are those having the branched beta-GlcNAc-(1----6)[beta-Gal- (1----3)]-GalNAcol core, and others with the following linear sequences not characterised previously from this source: beta-Gal-(1----3)-beta-GlcNAc-(1----3)-beta-Gal-(1----3)-GalNAcol, beta-Gal-(1----4)-beta-GlcNAc-(1----3)-beta-Gal-(1----3)-GalNAcol, alpha-GalNAc- (1----3)-beta-Gal-(1----3/4)-beta-GlcNAc-(1----3)-GalNAcol, beta-GlcNAc- (1----3)-beta-Gal-(1----3/4)-beta-GlcNAc-(1----3)- GalNAcol, beta-Gal-(1----3/4)-beta-GlcNAc-(1----3)-beta-Gal-(1----3/4)-beta- GlcNAc-(1----3)-beta-Gal-(1----3)-GalNAcol, and beta-GlcNAc-(1----3)-beta-Gal-(1----3/4)-beta-GlcNAc-(1----3)-beta-Gal- (1----3/4)-beta-GlcNAc-(1----3)-GalNAcol.


Assuntos
Glicolipídeos/química , Glicoproteínas/química , Mecônio/química , Oligossacarídeos/química , Antocianinas , Sequência de Carboidratos , Cromatografia em Camada Fina , Humanos , Recém-Nascido , Espectroscopia de Ressonância Magnética , Espectrometria de Massas , Dados de Sequência Molecular , Coloração e Rotulagem
16.
Clin Exp Immunol ; 85(3): 536-41, 1991 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-1716543

RESUMO

The specificities of seven monoclonal antibodies to the human B cell differentiation marker CD24 have been investigated with respect to sialic acid containing carbohydrates. These are antibodies HB8, HB9, VIB-C5, VIB-E3, AL1a, LC66 and IB5, which are known to bind to polydisperse sialoglycoprotein(s) on Nalm-6 B lymphoblastoid cells. Three of the antibodies, HB9, VIB-E3 and IB5, have been found to resemble the first described antibody in this series, BA-1, in binding also to bovine submaxillary mucin. As with BA-1, the binding of the antibodies is abolished or reduced markedly after desialylation of the epithelial glycoprotein, and the binding to neuraminidase-treated Nalm-6 cells is also reduced. There is evidence for the involvement of of non-O-acetylated sialic acid in the determinants recognized by these antibodies, since there is a substantially enhanced binding following mild-alkali treatment of the epithelial mucin which removes O-acetyl groups. One of the antibodies, VIB-E3, is deduced to recognize the oligosaccharide sequences NeuAc alpha 2-6GalNAc and NeuAc alpha 2-6Gal as part of larger antigenic structures. This conclusion has been reached from the results of inhibition-of-binding experiments using a series of structurally defined sialo-oligosaccharides and direct binding experiments using oligosaccharides chemically linked to lipid (neoglycolipids).


Assuntos
Anticorpos Monoclonais/imunologia , Antígenos CD/imunologia , Glicoproteínas de Membrana , Ácidos Siálicos/farmacologia , Animais , Antígeno CD24 , Sequência de Carboidratos , Bovinos , Epitopos/imunologia , Glicolipídeos/metabolismo , Glicopeptídeos/farmacologia , Humanos , Dados de Sequência Molecular , Ácido N-Acetilneuramínico , Oligossacarídeos/imunologia , Oligossacarídeos/farmacologia , Ligação Proteica , Sialoglicoproteínas/metabolismo , Glândula Submandibular
17.
Carbohydr Res ; 213: 293-307, 1991 Jun 25.
Artigo em Inglês | MEDLINE | ID: mdl-1933944

RESUMO

The aim of the present study has been to investigate the binding specificity of the soluble 14,000-dalton lectin of bovine heart muscle towards immobilised oligosaccharides in clustered form. To this end, chromatogram overlay assays and quantitative plastic-microwell-binding assays have been performed using several natural glycolipids and neoglycolipids containing one or more of the disaccharide units, beta-D-Galp-(1----4 or 3)-D-GlcNAc or beta-D-Galp-(1----4)-D-Glc and related structures. The microwell assay gave the most consistent results. It was observed that for binding by the soluble lectin the optimal sequence, which is beta-D-Galp-(1----4 or 3)-D-GlcNAc, must occur at the nonreducing end of longer oligosaccharides when linked to lipid. These oligosaccharides may be of poly(N-acetyllactosamine) type or they may be mono- or multi-antennary, complex-type chains in which the disaccharide is joined directly to a trimannosyl core. The lectin bound to such immobilised lipid-linked oligosaccharides on which the terminal D-galactosyl groups are substituted with alpha-L-Fucp-(1----2), alpha-D-Galp-(1----3), or alpha-NeuAc-(2----3) groups. However, no binding was detected if the terminal D-galactosyl groups were substituted with an alpha-NeuAc-(2----6) group or the subterminal N-acetylglucosamine units with an alpha-L-Fucp-(1----3 or -4) group. Internally located N-acetyllactosamine units where the D-galactose units are disubstituted by beta-D-GlcNacp-(1----3) and -(1----6) units, as in branched poly(N-acetyllactosamine) backbones were not bound by the bovine lectin. These results are in accord with previous observations on the bovine lectin and the corresponding human and rat lectins, using structurally defined oligosaccharides as inhibitors of binding. The results of comparative binding experiments using paragloboside and ceramide hexasaccharide which contain one and two N-acetyllactosamine units, respectively, joined in linear sequence to the lactosylceramide core, were equivocal with respect to the availability of the internal N-acetyllactosamine units for binding by the bovine lectin.


Assuntos
Hemaglutininas/metabolismo , Lectinas/metabolismo , Animais , Sítios de Ligação , Configuração de Carboidratos , Sequência de Carboidratos , Bovinos , Galectinas , Glicolipídeos/química , Glicolipídeos/metabolismo , Hemaglutininas/química , Técnicas In Vitro , Lectinas/química , Dados de Sequência Molecular , Peso Molecular , Proteínas Musculares/química , Proteínas Musculares/imunologia , Proteínas Musculares/metabolismo , Miocárdio/imunologia , Miocárdio/metabolismo , Solubilidade
18.
Biol Mass Spectrom ; 20(5): 313-23, 1991 May.
Artigo em Inglês | MEDLINE | ID: mdl-1883867

RESUMO

Product ion mass spectral data of [M + H]+ ions of oligosaccharides, mainly tetra- and pentasaccharides, as their dipalmitoyl phosphatidylethanolamine derivatives were obtained using both liquid secondary ion mass spectrometry with B/E linked scanning and fast atom bombardment ionization with collision-induced dissociation/tandem mass spectrometry. Both methods give similar positive product ion spectra of equivalent high sensitivity (detection limits of approximately 50 pmol) that principally contain glycosidic cleavage ions retaining the reducing end of the molecule from which monosaccharide sequence can be deduced. A series of ions from fission of the phosphate ester bond together with glycosidic cleavage are present in the tandem mass spectra and B/E linked scan spectra when helium collision gas is used. Monosaccharide linkage position of isomeric molecules is reflected in the intensity of glycosidic fragmentation, without retention of the oxygen atom, with decreasing cleavage in the order 1-3 greater than 1-4 greater than 1-6 linkage. Fucose and N-acetylhexosamines show an increased degree of fragmentation over hexose sugars. The application of product ion spectra of derivatized oligosaccharides is demonstrated for characterizing mixed samples and also the acquisition of spectra directly from the silica surface of high-performance thin-layer chromatography plates.


Assuntos
Oligossacarídeos/química , Fosfatidiletanolaminas/química , Espectrometria de Massas/métodos
19.
Eur J Biochem ; 189(3): 499-507, 1990 May 20.
Artigo em Inglês | MEDLINE | ID: mdl-2161756

RESUMO

In the course of characterising neoglycolipid products derived from mucin oligosaccharide alditols after periodate oxidation and coupling by reductive amination to the aminolipid dipalmitoylglycerophosphoethanolamine, we have obtained evidence that oxidative cleavage occurs specifically at the C4-C5 bond of core N-acetylgalactosaminitol. Two lipid-linked fragments thus obtained from each oligosaccharide alditol are well resolved on thin-layer chromatography and can be sensitively analysed by liquid-secondary-ion mass spectrometry to assign the sequence and branching patterns of oligosaccharides linked at C6 and C3 to the N-acetylgalactosamitol. These conclusions have been reached from detailed studies of the neoglycolipid derivatives of several oligosaccharides (di- to hexasaccharides) which were isolated from human meconium and characterised previously by MS and NMR studies as the free alditols.


Assuntos
Glicolipídeos/análise , Oligossacarídeos/análise , Fosfolipídeos/análise , Álcoois Açúcares/análise , Aminação , Configuração de Carboidratos , Sequência de Carboidratos , Cromatografia em Camada Fina , Humanos , Espectrometria de Massas , Mecônio/análise , Microquímica , Dados de Sequência Molecular , Oxirredução , Ácido Periódico
20.
Carbohydr Res ; 200: 47-57, 1990 Apr 25.
Artigo em Inglês | MEDLINE | ID: mdl-2199041

RESUMO

The sensitivity of detection and extent of information on structure obtainable by liquid-secondary-ion mass spectrometry (l.s.i.-m.s.) of neoglycolipids on the conventional target probe and directly from the surface of silica plates following t.l.c. has been assessed. Neoglycolipids were derived from malto-oligosaccharides, chitin oligosaccharides, and a range of deoxyhexose-, hexose-, 2-acetamido-2-deoxyhexose-, and sialic acid-containing mammalian oligosaccharides by reductive amination using phosphatidylethanolamine dipalmitoate (PPEADP). Sub-pmol amounts of the maltopentaose-PPEADP derivative applied directly to the target probe provided information on molecular weight, whereas approximately 1 pmol was required when analysed on the silica gel t.l.c. plate. With a biantennary octasaccharide derivative, the sensitivity of detection was 20-50 times lower and the other oligosaccharides had intermediate sensitivities. Information on composition and sequence was obtained readily from fragment ions, using 5 pmol of the maltopentaose derivative and 50 pmol of the octasaccharide derivative on the target probe, and 50 and 200 pmol, respectively, on the silica gel chromatogram. The optimised conditions formed the basis for characterising the structures of the components of mixtures of oligosaccharides generated from glycoproteins.


Assuntos
Glicolipídeos , Oligossacarídeos , Configuração de Carboidratos , Sequência de Carboidratos , Fenômenos Químicos , Química , Espectrometria de Massas , Dados de Sequência Molecular , Fosfatidiletanolaminas , Sensibilidade e Especificidade
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