RESUMO
Taurine administered during hypoxia reduced the cell damage due to O2 deficiency markedly. The beneficial effect outlasted the period of reoxygenation. The mechanisms for the improved survival rates are postulated in a reduced osmoregulatory disturbance of cellular integrity, improved Ca2+ homeostasis and induction of accelerated cellular growth processes. We conclude that taurine supplementation of the conventionally used kidney preservation solution (UW) improves this "gold standard" kidney preservation solution markedly.
Assuntos
Hipóxia Celular , Transplante de Rim , Rim/citologia , Soluções para Preservação de Órgãos , Taurina/farmacologia , Preservação de Tecido/métodos , Nucleotídeos de Adenina/metabolismo , Adenosina , Aerobiose , Alopurinol , Animais , Sobrevivência Celular/efeitos dos fármacos , Transplante de Células , Metabolismo Energético , Glutationa , Insulina , Rim/efeitos dos fármacos , Rim/metabolismo , Túbulos Renais , L-Lactato Desidrogenase , Rafinose , SuínosRESUMO
We conclude that, within this experimental model and under these experimental conditions, taurine supplementation of standard kidney preservation solutions improves survival of kidney cells during hypoxic preservation. The protective effect depends on the taurine concentration, the hypoxic preservation time and the used preservation solution. Physiological taurine concentrations are effective during short hypoxic periods, whereas pharmacological taurine concentrations seem to be needed for longer periods of hypoxia. Within this experimental model University of Wisconsin solution seems to be more effective than Euro collins solution.
