The Accuracy of Dental Students and Dentists Assessing the Total Occlusal Convergence of Crown Preparations.

This study examined the ability of dental students and qualified dentists to visually assess the total occlusal convergence of clinical crowns. 10 working casts of maxillary molar crown preparations were installed in phantom heads in a clinical skills laboratory. 87 participants assessed the buccolingual and mesiodistal total occlusal convergence of the casts. 2nd-year students with no experience in fixed prosthodontics served as a control group with 3rd, 4th and 5th-year students and qualified dentists including specialists in restorative dentistry involved. Differences were calculated between the measured angles and the subject's estimate to assess accuracy. For 8 of the 20 surfaces measured there were significant differences. (p ⟨ 0.05). Differences were not standardized between the groups, with no clear patterns of difference in tooth type or aspect assessed. Differences were primarily between the control group and the more experienced groups. The remaining groups were of similar ability. A minority of participants in each group were highly divergent from their peers. This study found significant differences between participants with no experience of fixed prosthodontics and those with experience, however, the ability to assess total occlusal convergence does not appear to improve significantly with experience.

Adequately Prepared? A Study Using an Innovative Computer Application to Measure Clinical Crown Convergence Angles Achieved by Students at a UK Dental School.

This study examined the total occlusal convergence angles created for full coverage crown preparations by students at a UK dental school. Working casts of 82 clinical crown preparations were scanned using a 3D scanner. Stereolithographic files were uploaded to Preppr, a crown preparation analysis application. Mean bucco-lingual convergence angle were 19.6° (+/-11.7) and mesial-distally 17.8° (+/-11.1). Smallest bucco-lingual convergence angles were achieved for canine teeth with the largest on molar teeth. The smallest mesio-distal values were on canine teeth with the largest on molar teeth. Ideal total convergence angles (4-14°) were achieved in 23% of bucco-lingual preparations and 33% of mesio-distal preparation. Results for clinically acceptable angles (10-20°) were 30% and 40% respectively. There were no statistically significant differences between tooth types for mean bucco-lingual values. (p=0.623), mesio-distal mean values were statistically different by tooth type (p=0.003). Mean values for mandibular molars were significantly higher than for maxillary incisors (p=0.001) and mandibular molars had significantly higher values than maxillary canines (p=0.045). Results in this study were comparable to those of other students and qualified clinicians, with a minority of preparation achieving ideal values.

Matrix heater in the gravitational wave observatory GEO 600.

Large scale laser interferometric gravitational wave detectors (GWDs), such as GEO 600 require high quality optics to reach their design sensitivity. The inevitable surface imperfections, inhomogeneities, and light-absorption induced thermal lensing in the optics, can convert laser light from the fundamental mode to unwanted higher order modes, and pose challenges to the operation and sensitivity of the GWDs. Here we demonstrate the practical implementation of a thermal projection system which reduces those unwanted effects via targeted spatial heating of the optics. The thermal projector consists of 108 individually addressable heating elements which are imaged onto the beam splitter of GEO 600. We describe the optimization of the spatial heating profile and present the obtained results.

Huddle test measurement of a near Johnson noise limited geophone.

In this paper, the sensor noise of two geophone configurations (L-22D and L-4C geophones from Sercel with custom built amplifiers) was measured by performing two huddle tests. It is shown that the accuracy of the results can be significantly improved by performing the huddle test in a seismically quiet environment and by using a large number of reference sensors to remove the seismic foreground signal from the data. Using these two techniques, the measured sensor noise of the two geophone configurations matched the calculated predictions remarkably well in the bandwidth of interest (0.01 Hz-100 Hz). Low noise operational amplifiers OPA188 were utilized to amplify the L-4C geophone to give a sensor that was characterized to be near Johnson noise limited in the bandwidth of interest with a noise value of 10-11 m/Hz at 1 Hz.

Damping and local control of mirror suspensions for laser interferometric gravitational wave detectors.

The mirrors of laser interferometric gravitational wave detectors hang from multi-stage suspensions. These support the optics against gravity while isolating them from external vibration. Thermal noise must be kept small so mechanical loss must be minimized and the resulting structure has high-Q resonances rigid-body modes, typically in the frequency range between about 0.3 Hz and 20 Hz. Operation of the interferometer requires these resonances to be damped. Active damping provides the design flexibility required to achieve rapid settling with low noise. In practice there is a compromise between sensor performance, and hence cost and complexity, and sophistication of the control algorithm. We introduce a novel approach which combines the new technique of modal damping with methods developed from those applied in GEO 600. This approach is predicted to meet the goals for damping and for noise performance set by the Advanced LIGO project.

Translational, rotational, and vibrational coupling into phase in diffractively coupled optical cavities.

All-reflective optical systems are under consideration for future gravitational wave detector topologies. A key feature of these all-reflective systems is the use of Fabry-Perot cavities with diffraction gratings as input couplers; however, theory predicts and experiment has shown that translation of the grating surface across the incident laser light will introduce additional phase into the system. This translation can be induced through simple side-to-side motion of the coupler, yaw motion of the coupler around a central point (i.e., rotation about a vertical axis), and even via internal resonances (i.e., vibration) of the optical element. In this Letter we demonstrate on a prototype-scale suspended cavity that conventional cavity length-sensing techniques used to detect longitudinal changes along the cavity axis will also be sensitive to translational, rotational, and vibrational motion of the diffractive input coupler. We also experimentally verify the amplitude response and frequency dependency of the noise coupling as given by theory.

Apparatus for dimensional characterization of fused silica fibers for the suspensions of advanced gravitational wave detectors.

Detection of gravitational waves from astrophysical sources remains one of the most challenging problems faced by experimental physicists. A significant limit to the sensitivity of future long-baseline interferometric gravitational wave detectors is thermal displacement noise of the test mass mirrors and their suspensions. Suspension thermal noise results from mechanical dissipation in the fused silica suspension fibers suspending the test mass mirrors and is therefore an important noise source at operating frequencies between ∼10 and 30 Hz. This dissipation occurs due to a combination of thermoelastic damping, surface and bulk losses. Its effects can be reduced by optimizing the thermoelastic and surface loss, and these parameters are a function of the cross sectional dimensions of the fiber along its length. This paper presents a new apparatus capable of high resolution measurements of the cross sectional dimensions of suspension fibers of both rectangular and circular cross section, suitable for use in advanced detector mirror suspensions.

Invited article: CO2 laser production of fused silica fibers for use in interferometric gravitational wave detector mirror suspensions.

In 2000 the first mirror suspensions to use a quasi-monolithic final stage were installed at the GEO600 detector site outside Hannover, pioneering the use of fused silica suspension fibers in long baseline interferometric detectors to reduce suspension thermal noise. Since that time, development of the production methods of fused silica fibers has continued. We present here a review of a novel CO(2) laser-based fiber pulling machine developed for the production of fused silica suspensions for the next generation of interferometric gravitational wave detectors and for use in experiments requiring low thermal noise suspensions. We discuss tolerances, strengths, and thermal noise performance requirements for the next generation of gravitational wave detectors. Measurements made on fibers produced using this machine show a 0.8% variation in vertical stiffness and 0.05% tolerance on length, with average strengths exceeding 4 GPa, and mechanical dissipation which meets the requirements for Advanced LIGO thermal noise performance.

Experimental demonstration of a suspended diffractively coupled optical cavity.

All-reflective optical systems are under consideration for future gravitational wave detector topologies. One approach in proposed designs is to use diffraction gratings as input couplers for Fabry-Perot cavities. We present an experimental demonstration of a fully suspended diffractively coupled cavity and investigate the use of conventional Pound-Drever-Hall length sensing and control techniques to maintain the required operating condition.

Optical modulation techniques for length sensing and control of optical cavities.

We present a discussion of the use of amplitude modulation techniques with regard to the length sensing and control of optical cavities for laser interferometric gravitational-wave detectors. Traditional radio-frequency amplitude modulation techniques automatically include phase modulation as a product of the modulation process, which can contaminate the signal after demodulation. In particular, with many length-sensing and control schemes the detected signals are demodulated in quadrature, which, in the case of a traditional amplitude modulation scheme, will result in offsets due to the additional phase modulation. We demonstrate this effect using a simple optical cavity configuration and show that minor adjustments to the modulator system can be used to compensate for the extra modulation components and provide additional flexibility.

LRRK2 mutations in Parkinson disease.

To determine the frequency of LRRK2 mutations in idiopathic Parkinson disease (PD), the authors studied 786 PD probands, 32 affected siblings, 1,044 unaffected siblings, and 278 unrelated controls. The authors designed allelic discrimination assays for nine LRRK2 mutations and identified these in six probands with PD, one affected sibling, one unaffected sibling, and one unrelated control. Thus LRRK2 mutations only rarely cause idiopathic PD.

A limited role for DJ1 in Parkinson disease susceptibility.

An association study of four common polymorphisms in the DJ1 gene and Parkinson disease (PD) was conducted. PD probands were compared with their unaffected siblings matched by gender and closest age at study (416 vs 416) and with unrelated control subjects (691 vs 190). None of the four haplotype tagging single-nucleotide polymorphisms (SNPs) was associated with PD overall, but SNP1 (position 4,345 bp) and SNP3 (position 16,491 bp) were associated with PD in women (p = 0.03 and p = 0.002).

Validity of family history data on PD: evidence for a family information bias.

OBJECTIVE: To study the validity of information provided by case and control subjects (or their proxies) about PD among their first-degree relatives. METHODS: Secondary cases of PD were assessed both through a single informant (family history method) and through the study of each relative (family study method). The family study method was considered as the standard for comparison, and the sensitivity and specificity of the family history method were studied. RESULTS: A total of 133 population-based case subjects and their 655 relatives were recruited, and 119 population-based control subjects and their 511 relatives. Sensitivity was 68% (95% CI = 47 to 85) for cases and 45% (95% CI = 17 to 77) for controls. Specificity was 99% (95% CI = 98 to 99) for cases and 100% (95% CI = 99 to 100) for controls. The odds ratio (OR) for family history of PD was 4.34 (95% CI = 1.63 to 11.58, p = 0.003) using the family history method and 1.86 (95% CI = 0.78 to 4.44, p = 0.16) using the family study method. The former significant OR more than doubled the latter not significant OR (relative bias = 133%). Bias was more pronounced for proxy interviews and for women informants, and when the relatives were siblings, were living, and were examined or had medical record documentation. CONCLUSIONS: Case subjects with PD (or their proxies) are more aware of PD among their first-degree relatives than control subjects (or their proxies); however, they overreport PD in relatives who are not affected. This causes a substantial family information bias.

The epidermal growth factor receptor is required to maintain the proliferative population in the basal compartment of epidermal tumors.

Previous studies using keratinocytes from epidermal growth factor receptor (EGFR)-deficient mice revealed that the EGFR is not required for papilloma formation initiated by a mutant rasHa gene, although the tumors that develop are very small (A. A. Dlugosz et aL, Cancer Res., 57: 3180-3188, 1997). The current study used a combination of bromodeoxyuridine pulse-chase, proliferating cell nuclear antigen distribution, and differentiation marker analysis to reveal the following: (a) the EGFR was required to maintain the proliferative population in the basal cell compartment of papillomas; (b) in the absence of EGFR, cycling tumor cells migrated into the suprabasal compartment and initiated the differentiation program prematurely; and (c) these changes were associated with cell cycle arrest. Further analysis of v-rasHa-transformed EGFR-deficient keratinocytes in vitro indicated that such cells migrated more on and attached less to extracellular matrix components. Together, these studies reveal that an essential function for the EGFR pathway in squamous tumors is to maintain a proliferative pool of basal cells and prevent premature terminal differentiation.

Response of a Fabry-Perot optical cavity to phase modulation sidebands for use in electro-optic control systems.

The worldwide endeavor to build long baseline laser interferometers to detect and study gravitational radiation is well under way. In the German-British GEO600 project, it is proposed to pass the sidebands induced on the light by an electro-optic phase modulator through a Fabry-Perot optical cavity used in transmission, called a mode cleaner. This can be achieved when the phase modulation frequency is matched to the first longitudinal-mode frequency of the mode cleaner cavity so that both carrier and sidebands are transmitted. The primary function of the mode cleaner is to reduce the geometry fluctuations associated with the light, and thus any such noise induced by the modulation process is also suppressed. We present the results of an experiment that investigates the feasibility of passing modulation sidebands through an optical cavity and the factors limiting its success. In particular, we show that it is possible to avoid introducing excess noise associated with the transmitted sidebands, provided that certain experimental criteria are satisfied. The research was carried out on a prototype mode cleaner cavity built and tested at Glasgow University but which is similar to the equivalent apparatus planned for GEO600.

Light scattering described in the mode picture.

A system of orthonormal functions representing the eigenmodes of an optical resonator with perfectly spherical mirror surfaces has been described in the literature. In real experiments, however, the wave front of the passing beam will be deformed by surface irregularities or index inhomogeneities inside components traversed by the beam. We describe quantitatively the relative power transferred out of the fundamental mode into higher-order modes by these irregularities.