RESUMO
BACKGROUND: Extracellular vesicles released by different cells have been isolated from diverse fluids including saliva. We previously reported that rat submandibular glands secrete nanovesicles that catalyze hydrolysis of ATP, ADP and AMP, which are actors of the purinergic signaling system along with adenosine. Extracellular nucleotides like ATP and adenosine are involved in the regulation of inflammatory processes and apoptosis. Histamine, a widely distributed biogenic amine, is involved in inflammatory response. OBJECTIVE: To test if activation of histamine receptors in rat submandibular gland promotes changes in the release of vesicles with nucleotidase activity that could modulate purinergic signaling. METHODS: Rat submandibular glands were incubated in the absence or presence of histamine and JNJ7777120, an antagonist for H4 receptors. Extracellular vesicles were isolated from incubation media by differential centrifugation. Vesicular nucleotidase activity was measured following Pi release by 3mM MgATP, MgADP or MgAMP. RESULTS: Histamine increased the release of vesicles with nucleotidase activity in a concentration dependent manner. JNJ7777120 significantly reduced this effect. Vesicular nucleotidases obtained in the absence or presence of histamine promoted Pi production from ATP, ADP and AMP. CONCLUSION: The results show a relationship between histamine and the regulation of purinergic signaling, which could be important in the modulation of inflammatory processes.
Assuntos
Vesículas Extracelulares/enzimologia , Histamina/farmacologia , Nucleotidases/metabolismo , Glândula Submandibular/metabolismo , Animais , Técnicas In Vitro , Indóis/farmacologia , Masculino , Microscopia Eletrônica de Transmissão , Piperazinas/farmacologia , Ratos , Ratos WistarRESUMO
Acute nociceptive, inflammatory, and neuropatic pain all depend to some degree on the peripheral activation of primary sensory afferent neurons. The prevent administration of drugs can potentially optimize drug concentrations at the site of origin of the pain... At present, the administration of nonsteroidal antiinflammatory drugs, opioids, local anesthetic are being used in a variety of clinical states.
Assuntos
Humanos , Analgésicos Opioides/administração & dosagem , Analgésicos Opioides/uso terapêutico , Anti-Inflamatórios não Esteroides , Dexametasona , Antagonistas dos Receptores Histamínicos H1 , Dor , Odontalgia , BetametasonaRESUMO
Acute nociceptive, inflammatory, and neuropatic pain all depend to some degree on the peripheral activation of primary sensory afferent neurons. The prevent administration of drugs can potentially optimize drug concentrations at the site of origin of the pain... At present, the administration of nonsteroidal antiinflammatory drugs, opioids, local anesthetic are being used in a variety of clinical states.(AU)
