Reflections on a measurement of the gravitational constant using a beam balance and 13 tons of mercury.

In 2006, a final result of a measurement of the gravi- tational constant G performed by researchers at the University of Zürich, Switzerland, was published. A value of G=6.674252(122)×10-11 m3 kg-1 s-2 was obtained after an experimental effort that lasted over one decade. Here, we briefly summarize the measurement and discuss the strengths and weaknesses of this approach.

New precise measurement of the pion weak form factors in pi+-->e+ nugamma decay.

We have measured the pi+-->e+ nugamma branching ratio over a wide region of phase space, based on a total of 65 460 events acquired using the PIBETA detector. Minimum-chi2 fits to the measured (E(e+), E(gamma) energy distributions result in the weak form factor value of F(A)=0.0119(1) with a fixed value of F(V)=0.0259. An unconstrained fit yields F(V)=0.0258(17) and F(A)=0.0117(17). In addition, we have measured a=0.10(6) for the dependence of F(V) on q2, the e+ nu pair invariant mass squared, parametrized as F(V)(q2)=F(V)(0)(1+aq(2)). The branching ratio for the kinematic region E(gamma)>10 MeV and theta(e(+)gamma)>40 degrees is measured to be B(expt)=73.86(54)x10(-8). Earlier deviations we reported in the high-E(gamma)-low-E(e+) kinematic region are resolved without a tensor term. We also derive new values for the pion polarizability alpha(E)=2.78(10)x10(-4) fm3 and neutral pion lifetime tau(pi0)=(8.5+/-1.1)x10(-17) s.

Erythropoietin gene expression in human, monkey and murine brain.

The haematopoietic growth factor erythropoietin is the primary regulator of mammalian erythropoiesis and is produced by the kidney and the liver in an oxygen-dependent manner. We and others have recently demonstrated erythropoietin gene expression in the rodent brain. In this work, we show that cerebral erythropoietin gene expression is not restricted to rodents but occurs also in the primate brain. Erythropoietin mRNA was detected in biopsies from the human hippocampus, amygdala and temporal cortex and in various brain areas of the monkey Macaca mulatta. Exposure to a low level of oxygen led to elevated erythropoietin mRNA levels in the monkey brain, as did anaemia in the mouse brain. In addition, erythropoietin receptor mRNA was detected in all brain biopsies tested from man, monkey and mouse. Analysis of primary cerebral cells isolated from newborn mice revealed that astrocytes, but not microglia cells, expressed erythropoietin. When incubated at 1% oxygen, astrocytes showed >100-fold time-dependent erythropoietin mRNA accumulation, as measured with the quantitative reverse transcription-polymerase chain reaction. The specificity of hypoxic gene induction in these cells was confirmed by quantitative Northern blot analysis showing hypoxic up-regulation of mRNA encoding the vascular endothelial growth factor, but not of other genes. These findings demonstrate that erythropoietin and its receptor are expressed in the brain of primates as they are in rodents, and that, at least in mice, primary astrocytes are a source of cerebral erythropoietin expression which can be up-regulated by reduced oxygenation.

[Epidermolysis bullosa in a calf]. / Epidermolysis bullosa bei einem Kalb.

A four-week-old Simmental x Red Holstein calf had lesions of skin and oral mucosa since its first days of life. Where skin or mucosa was exposed to minimal trauma as in oral cavity or over joints, it broke off, forming blisters and erosions. The diagnosis of epidermolysis bullosa, a hereditary disease, was based on clinical and histological findings. Histology pointed to the epidermal form of epidermolysis bullosa, comparable to epidermolysis bullosa simplex in humans.