RESUMO
BACKGROUND: Intranasal noninhaled delivery of carbon dioxide (CO2) is efficacious in the symptomatic treatment of seasonal allergic rhinitis. The goal of this study was to determine whether and how 100% CO2 inhibits mast cell degranulation, thereby possibly contributing to the reduction of symptoms in seasonal allergic rhinitis. METHODS: Peritoneal mast cells isolated from rats and labelled with sulforhodamine-B (SFRM-B) were used to determine whether CO2 treatment could block mast cell degranulation and histamine release in response to 48/80. In addition, the effect of CO2 on intracellular calcium levels in unstimulated and stimulated mast cells was determined by fluorescent microscopy. RESULTS: Treatment with 48/80 caused >90% of mast cells containing SFRM-B to degranulate, resulting in a marked decrease in the fluorescent intensity within the mast cells, and simultaneously causing a significant increase in histamine release. Significantly, the stimulatory effect of 48/80 on fluorescent intensity and histamine levels was greatly inhibited (>95%) to near control levels by pretreatment with 100% CO2. Treatment with 48/80 also caused a robust transient increase in intracellular calcium, whereas pretreatment with CO2 repressed the increase in calcium (>70%) in response to 48/80. CONCLUSIONS: Results from this study provide the first evidence of a unique regulatory mechanism by which CO2 inhibits mast cell degranulation and histamine release by repressing stimulated increases in intracellular calcium. Thus, our data provide a plausible explanation for the reported therapeutic benefit of noninhaled intranasal delivery of 100% CO2 to treat allergic rhinitis.
Assuntos
Cálcio/metabolismo , Dióxido de Carbono/farmacologia , Degranulação Celular/efeitos dos fármacos , Espaço Intracelular/efeitos dos fármacos , Mastócitos/efeitos dos fármacos , Animais , Células Cultivadas , Formaldeído/farmacologia , Liberação de Histamina/efeitos dos fármacos , Imunossupressores/farmacologia , Espaço Intracelular/metabolismo , Masculino , Mastócitos/metabolismo , Ratos , Ratos Sprague-Dawley , p-Metoxi-N-metilfenetilamina/farmacologiaAssuntos
Mandíbula/diagnóstico por imagem , Adulto , Humanos , Masculino , Mandíbula/anormalidades , RadiografiaRESUMO
In a review of the literature, it can be determined that large osteomas of the mandible are relatively rare. Of those that have been described, the location is normally posterior to the premolars on the lingual surface of the mandible or in the condylar area. This article presents a case of an atypical presentation of an osteoma arising from the genial tubercle area.
