Chronobiological analysis of peripheral lymphocyte dehydrogenase activities in rats with Walker 256 carcinosarcoma.

Alpha-glycerophosphate dehydrogenase (alpha-GPD) and succinate dehydrogenase (SD) activities were investigated in lymphocytes from peripheral blood of normal rats and rats bearing the Walker 256 carcinosarcoma. Computer analysis using an original algorithm revealed a hierarchy of biorhythmic patterns of dehydrogenase activities. In all rats, mean SD activity was higher than mean alpha-GPD activity. In rats without tumor, SD and alpha-GPD activities were both higher than in rats with the Walker tumor. Biorhythm spectra for both dehydrogenases were very similar in rats with or without tumor, but tumor implantation resulted in a change of the phase relationship between alpha-GPD and SD.

[A chronobiological analysis of the lymphocyte dehydrogenase activity in the peripheral blood of rats with Walker 256 carcinosarcoma]. / Khronobiologicheskii analiz aktivnosti degidrogenaz limfotsitov perifericheskoi krovi krys s kartsinosarkomoi Uoker 256.

In a series of experiments the alpha-glycerophosphate dehydrogenase and succinate dehydrogenase (SDH) activities were daily determined simultaneously by the cytochemical method in peripheral blood lymphocytes of normal rats and those with the Walker carcinosarcoma. Using an original algorithm a hierarchy of biorhythms was singled out by a computer according to individual changes in the dehydrogenase activity for each rat. The mean level of succinate dehydrogenase activity was higher than that of alpha-glycerophosphate dehydrogenase. A set of discovered biorhythms and their representation were identical in the both enzymes. The tumor transplantation caused changes in the phase coordination of investigated dehydrogenases in all rhythms and promoted an increase of the succinate dehydrogenase amplitude as compared with the alpha-glycerophosphate dehydrogenase amplitude in certain rhythms. It is concluded that biorhythmological parameters of these enzymes may be used for characterization of the tumor-bearing host.

[Biorhythm study of the alpha-glycerophosphate dehydrogenase activity of the peripheral blood lymphocytes in rats with Walker carcinosarcoma]. / Bioritmologicheskoe issledovanie aktivnosti al'fa-glitserofosfatdegidrogenazy limfotsitov perifericheskoi krovi krys s kartsinosarkomoi Uokera.

In the series of experiments alpha-glycerophosphate dehydrogenase activity was daily determined at the same time by cytochemical method in the peripheral lymphocyte of normal rats and those with Walker carcinosarcoma. Using an original algorithm a hierarchy of biorhythms was singled out on a computer according to the individual changes in alpha-glycerophosphate dehydrogenase activity in each rat. It was shown that tumor development resulted in the reduction of the mean level of alpha-glycerophosphate dehydrogenase activity and considerable changes in diverse rhythms. Particular attention is paid to the phase changes, which can reveal changes in phase coordination of glycerophosphate shuttle cycle components.

[The participation of the adrenal catecholamine system of tumor-bearing rats in sarcolysin-mediated effect]. / Ob uchasti sistemy katekholaminov nadpochechnikov krys-opukholenositelei v oposredovannom deistvii sarkolizina.

In rats with transplantable Walker carcinosarcoma the injection of sarcolysin is found to retard a fall in the adrenaline concentration in adrenal tissues, observed without the substance. Normalization of the adrenaline level proceeds against the background of variations in the noradrenaline concentration near and above control values. Injecting of sarcolysin in intact animals results in normalization of the adrenaline level against the background of a considerable fall in the noradrenaline concentration after each injection of the drug. Changes of the same character were also observed in case of injecting saline solution in intact animals, the only difference being due to the fact that the effect of sarcolysin is dependent on animals age, while that of saline solution is not.