RESUMO
Eserine (1-2 mg/kg) decreased incorporation of the radioactive precursors into total DNA of the rat liver by 40-50%. Only high eserine cincentrations (10-5 M) effected directly the DNA synthesis in the hepatocyte nuclei. It was shown in vitro that the DNA synthesis in the nuclei of hepatocytes, isolated from the liver of rats after eserine administration (1 mg/kg), decreased significantly. A conclusion is drawn that the eserine action on the incorporation of radioactive precursors into DNA of the rat liver in vivo is localized at the level of the DNA synthesis in the nuclei. The study of the acetylcholine action on the DNA synthesis in the isolated nuclei of hepatocytes suggests that the inhibitory effect of eserine on the DNA synthesis is mediated through acetylcholine.
Assuntos
DNA/biossíntese , Fígado/metabolismo , Fisostigmina/farmacologia , Acetilcolina/farmacologia , Animais , Depressão Química , Relação Dose-Resposta a Droga , Fígado/efeitos dos fármacos , Masculino , RatosRESUMO
Intoxication caused by intramuscular injection of picrotoxin and eserine (5 and 10 mg/kg) to albino rats is found to increase the degree of coupling of oxidation to phosphorylation and the rate of succinate oxidation. The conclusion may be drawn that the reaction of the oxidative phosphorylation system to the injection of the above pharmacological agents is not specific but reflects the compensatory shift in the microchondria functioning in response to hypoxia and seizures accompanying the intoxication.
Assuntos
Metabolismo Energético/efeitos dos fármacos , Mitocôndrias Hepáticas/metabolismo , Fisostigmina/farmacologia , Picrotoxina/farmacologia , Animais , Masculino , Mitocôndrias Hepáticas/efeitos dos fármacos , Fosforilação Oxidativa/efeitos dos fármacos , Ratos , Succinatos/metabolismoRESUMO
The influence of central cholinolytic cyclosil on the oxidative phosphorilation, ATPase activity and ultrastructure of hepatic mitochondria has been studied. The increased effectivity of mitochondrial macroergic compounds formation in the presence of cyclosil (0.04 mg/ml) was noticed. The oxygen consumption rate of these particles did not change. These findings and the electron microscopic observations on mitochondrial swelling in the presence of the cholinolytic agent suggest that cyclosil enchances the degree of mitochondrial energization.
Assuntos
Mitocôndrias Hepáticas/efeitos dos fármacos , Parassimpatolíticos/farmacologia , Fenetilaminas/farmacologia , Adenosina Trifosfatases/metabolismo , Animais , Ciclopentanos/farmacologia , Ativação Enzimática/efeitos dos fármacos , Feminino , Masculino , Mitocôndrias Hepáticas/metabolismo , Mitocôndrias Hepáticas/ultraestrutura , Fosforilação Oxidativa/efeitos dos fármacos , RatosRESUMO
Incorporation of labelled precursors into total and nuclear DNA of liver tissue was inhibited after administration into animals of eserine at a dose 0.1--0.2 mg/100 g of body weight. Simultaneously, DNAase activity was increased and cholinesterase activity was inhibited in liver tissue. The DNA synthesis was inhibited in liver slices, if they were incubated with 2.10(-6) M eserine. The DNAase activity was unaltered in vitro. A cholinolytic compound metacin, pre-administered into the animals, was ineffective at dose of 1 mg/kg; it slightly decreased the effect of eserine at dose of 0.25 mg/kg.
Assuntos
DNA/biossíntese , Fígado/efeitos dos fármacos , Fisostigmina/farmacologia , Acetilcolina/fisiologia , Animais , Colinesterases/metabolismo , Depressão Química , Relação Dose-Resposta a Droga , Técnicas In Vitro , Fígado/metabolismo , Masculino , Ratos , Timidina/metabolismo , Timina/metabolismoRESUMO
The effectiveness of macroergic compounds formation was found to increase in the mitochondria of the rat liver incubated in the presence of cyclosil (0.04 mg/ml). The rate of O2 consumption was unchanged. A tendency to increase the K+ transport into mitochondria in the presence of cyclosil was noted. The data obtained testify to an increase of the mitochondrial energization in the presence of cyclosil.