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Development ; 124(22): 4595-603, 1997 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-9409676

RESUMO

Spermatogenic cells synthesize a unique 70-kDa heat shock protein (HSP70-2) during prophase of meiosis I, and targeted disruption of the Hsp70-2 gene has shown that this protein is required for spermatogenic cell differentiation in adult mice. HSP70-2 is associated with synaptonemal complexes formed between paired homologous chromosomes during meiotic prophase. The present study focuses on the nearly synchronous first wave of spermatogenesis in 12- to 28-day old juvenile mice to determine more precisely when HSP70-2 is required and what meiotic processes are affected by its absence. Spermatogenesis in homozygous mutant mice (Hsp70-2[-/-]) proceeded normally until day 15 when increasing numbers of pachytene spermatocytes became apoptotic and differentiation of cells beyond the pachytene stage began to falter. Synaptonemal complexes assembled in Hsp70-2(-/-) mice and spermatocytes developed through the final pachytene substage. However, synaptonemal complexes failed to desynapse and normal diplotene spermatocytes were not observed. Metaphase spermatocytes were not seen in tissue sections from testes of Hsp70-2(-/-) mice, and expression of mRNAs and antigens characteristic of late pachytene spermatocytes (e.g., cyclin A1) and development of spermatids did not occur. Thus, HSP70-2 is required for synaptonemal complex desynapsis, and its absence severely impairs the transition of spermatogenic cells through the late meiotic stages and results in apoptosis beginning with the first wave of germ cell development in juvenile mice.


Assuntos
Proteínas de Choque Térmico HSP70/metabolismo , Espermatócitos/metabolismo , Animais , Apoptose , Sequência de Bases , Diferenciação Celular , Primers do DNA/genética , Proteínas de Choque Térmico HSP70/genética , Masculino , Meiose , Camundongos , Camundongos Knockout , Reação em Cadeia da Polimerase , Túbulos Seminíferos/anormalidades , Túbulos Seminíferos/metabolismo , Espermatócitos/ultraestrutura , Espermatogênese/genética , Espermatogênese/fisiologia , Complexo Sinaptonêmico/fisiologia , Testículo/anormalidades , Testículo/metabolismo
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