RESUMO
Development of preclinical dog models of solid organ and hematopoietic transplantation is critically dependent upon characterization of the polymorphic major histocompatibility complex class I and class II loci. While the class II alleles are easily typed as the polymorphic positions reside on a single exon, typing the class I locus is tedious. We have improved the class I typing method by designing improved primers and adopting alternative DNA amplification and cloning reagents that circumvent the use of radioactivity and the need for the single-stranded conformation polymorphism gels. The method is reliable in typing dogs for the class I dog leukocyte antigen (DLA)-88 locus, and through its use, we describe here two new alleles DLA-88*01101 and DLA-88*01201.
Assuntos
Alelos , Cães/genética , Cães/imunologia , Antígenos de Histocompatibilidade Classe I/genética , Leucócitos/imunologia , Animais , Sequência de Bases , Éxons , Teste de Histocompatibilidade/veterinária , Íntrons , Dados de Sequência Molecular , Filogenia , Polimorfismo GenéticoAssuntos
Hipercalcemia/tratamento farmacológico , Fosfatos/administração & dosagem , Complicações na Gravidez/tratamento farmacológico , Adenoma/complicações , Administração Oral , Adulto , Feminino , Humanos , Hipercalcemia/etiologia , Neoplasias das Paratireoides/complicações , Fosfatos/uso terapêutico , GravidezRESUMO
PIP: 181 cases of patients (aged 12-43) who underwent a second trimester abortion (14-20 weeks gestation) by intraamniotic hypertonic saline or prostaglandin F2 alpha are presented for the purpose of evaluating methodologies. 61 patients had hypertonic saline infusion, 37 with Laminaria tents inserted and 24 without. 1 of the 24 cases represents a method failure which was completed surgically 2 days after infusion. Of the 37 patients treated with saline and Laminaria tent, there were no method failures. 122 patients had a prostaglandin infusion and Laminaria tent insertion, and 22 also had intraamniotic calcium gluconate. The 22 cases who received an intraamniotic prostaglandin and calcium infusion with a Laminaria tent had no failures. The prostaglandin group demonstrated a shorter infusion-abortion time (average, 17 hours). When calcium was added to prostaglandin, no potentiating effect was noted. Major complications, most notably retained placenta, were noted with the saline method. Prostaglandin proved the most effective and least risky procedure.^ieng
Assuntos
Aborto Legal , Aborto Legal/métodos , Adolescente , Adulto , Cálcio , Criança , Feminino , Humanos , Gravidez , Segundo Trimestre da Gravidez , Prostaglandinas F , Solução Salina Hipertônica , Fatores de TempoRESUMO
Endometriosis of laparotomy scars has been described as rare. Interruption of pregnancy by abdominal hysterotomy affords an opportunity for its development. The two cases which are reported were admitted to the hospital within one month, which suggests that this diagnosis may not be rare.
