The potential of cold-adapted microorganisms for biodegradation of bioplastics.

Due to the extensive use of plastics, their quantity in the environment is constantly increasing, which creates a global problem. In the present study, we sought to isolate, test and identify Antarctic microorganisms which possess the ability to biodegrade bioplastics such as poly(ε-caprolactone) (PCL), poly(butylene succinate) (PBS) and poly(butylene succinate-co-butylene adipate) (PBSA) at low temperatures. 161 bacterial and 38 fungal isolates were isolated from 22 Antarctic soil samples. Among them, 92.16% of bacterial and 77.27% of fungal isolates formed a clear zone on emulsified PBSA, 98.04% and 81.82% on PBS and 100% and 77.27% on PCL as an additive to minimal medium at 20 °C. Based on the 16S and 18S rRNA sequences, bacterial strains were identified as species belonging to Pseudomonas and Bacillus and fungal strains as species belonging to Geomyces, Sclerotinia, Fusarium and Mortierella, while the yeast strain was identified as Hansenula anomala. In the biodegradation process conducted under laboratory conditions at 14, 20 and 28 °C, Sclerotinia sp. B11IV and Fusarium sp. B3'M strains showed the highest biodegradation activity at 20 °C (49.68% for PBSA and 33.7% for PCL, 45.99% for PBSA and 49.65% for PCL, respectively). The highest biodegradation rate for Geomyces sp. B10I was noted at 14 °C (25.67% for PBSA and 5.71% for PCL), which suggested a preference for lower temperatures (at 20 °C the biodegradation rate was only 11.34% for PBSA, and 4.46% for PCL). These data showed that microorganisms isolated from Antarctic regions are good candidates for effective plastic degradation at low temperatures.

Drift-dependent changes in iceberg size-frequency distributions.

Although the size-frequency distributions of icebergs can provide insight into how they disintegrate, our understanding of this process is incomplete. Fundamentally, there is a discrepancy between iceberg power-law size-frequency distributions observed at glacial calving fronts and lognormal size-frequency distributions observed globally within open waters that remains unexplained. Here we use passive seismic monitoring to examine mechanisms of iceberg disintegration as a function of drift. Our results indicate that the shift in the size-frequency distribution of iceberg sizes observed is a product of fracture-driven iceberg disintegration and dimensional reductions through melting. We suggest that changes in the characteristic size-frequency scaling of icebergs can be explained by the emergence of a dominant set of driving processes of iceberg degradation towards the open ocean. Consequently, the size-frequency distribution required to model iceberg distributions accurately must vary according to distance from the calving front.

Isolation and characterization of Arctic microorganisms decomposing bioplastics.

The increasing amount of plastic waste causes significant environmental pollution. In this study, screening of Arctic microorganisms which are able to degrade bioplastics was performed. In total, 313 microorganisms were isolated from 52 soil samples from the Arctic region (Spitsbergen). Among the isolated microorganisms, 121 (38.66%) showed biodegradation activity. The ability of clear zone formation on emulsified poly(butylene succinate-co-adipate) (PBSA) was observed for 116 microorganisms (95.87%), on poly(butylene succinate) (PBS) for 73 microorganisms (60.33%), and on poly(ɛ-caprolactone) (PCL) for 102 microorganisms (84.3%). Moreover, the growth of microorganisms on poly(lactic acid) (PLA) agar plates was observed for 56 microorganisms (46.28%). Based on the 16S rRNA sequence, 10 bacterial strains which showed the highest ability for biodegradation were identified as species belonging to Pseudomonas sp. and Rhodococcus sp. The isolated fungal strains were tested for polycaprolactone films and commercial corn and potato starch bags degradation under laboratory conditions. Strains 16G (based on the analysis of a partial 18S rRNA sequence, identified as Clonostachys rosea) and 16H (identified as Trichoderma sp.) showed the highest capability for biodegradation. A particularly high capability for biodegradation was observed for the strain Clonostachys rosea, which showed 100% degradation of starch films and 52.91% degradation of PCL films in a 30-day shake flask experiment. The main advantage of the microorganisms isolated from Arctic environment is the ability to grow at low temperature and efficient biodegradation under this condition. The data suggest that C. rosea can be used in natural and laboratory conditions for degradations of bioplastics.